Bahun: Inhibition of the SARS-CoV-2 3CLpro main protease by plant polyphenols [Quercetin]

Inhibition of the SARS-CoV-2 3CLpro main protease by plant polyphenols

Bahun et al., Food Chemistry, doi:10.1016/j.foodchem.2021.131594 (In Vitro)

Bahun et al., Inhibition of the SARS-CoV-2 3CLpro main protease by plant polyphenols, Food Chemistry, doi:10.1016/j.foodchem.2021.131594 (In Vitro)

Nov 2021 Source PDF

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In Silico and In Vitro study of plant polyphenols identifying quercetin, curcumin, ellagic acid, epigallocatechin gallate and resveratrol as SARS-CoV-2 3CL^pro inhibitors with IC₅₀ between 11.8µM and 23.4µM. Real-time binding was analyzed with surface plasmon resonance spectroscopy.

7 In Vitro studies support the efficacy of quercetin [Aguado, Bahun, Goc, Kandeil, Munafò, Singh, Xu].

Important missing comments or errors? Let us know.

This study includes quercetin and curcumin.

1. Aguado et al., bioRxiv, doi:10.1101/2023.01.17.524329, Senolytic therapy alleviates physiological human brain aging and COVID-19 neuropathology, https://www.biorxiv.org/content/10.1101/2023.01.17.524329.

2. Bahun et al., Food Chemistry, doi:10.1016/j.foodchem.2021.131594, Inhibition of the SARS-CoV-2 3CLpro main protease by plant polyphenols, https://www.sciencedirect.com/science/article/pii/S0308814621026005.

3. Goc et al., European Journal of Microbiology and Immunology, doi:10.1556/1886.2021.00022, Inhibitory effects of specific combination of natural compounds against SARS-CoV-2 and its Alpha, Beta, Gamma, Delta, Kappa, and Mu variants, https://akjournals.com/view/journa..rticle-10.1556-1886.2021.00022.xml.

4. Kandeil et al., Pathogens, doi:10.3390/pathogens10060758, Bioactive Polyphenolic Compounds Showing Strong Antiviral Activities against Severe Acute Respiratory Syndrome Coronavirus 2, https://www.mdpi.com/2076-0817/10/6/758.

5. Munafò et al., Research Square, doi:10.21203/rs.3.rs-1149846/v1, Quercetin and Luteolin Are Single-digit Micromolar Inhibitors of the SARS-CoV-2 RNA-dependent RNA Polymerase, https://www.researchsquare.com/article/rs-1149846/v1.html.

6. Singh et al., Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, doi:10.1016/j.bbadis.2021.166322, The spike protein of SARS-CoV-2 virus induces heme oxygenase-1: Pathophysiologic implications, https://www.sciencedirect.com/science/article/pii/S0925443921002556.

7. Xu et al., Proceedings of the National Academy of Sciences, doi:10.1073/pnas.2301775120, Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory effects against COVID-19, https://pnas.org/doi/10.1073/pnas.2301775120.

Bahun et al., 14 Nov 2021, peer-reviewed, 10 authors.

In Vitro studies are an important part of preclinical research, however results may be very different in vivo.

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Inhibition of the SARS-CoV-2 3CLpro main protease by plant polyphenols

Miha Bahun, Marko Jukić, Domen Oblak, Luka Kranjc, Gregor Bajc, Matej Butala, Krištof Bozovičar, Tomaž Bratkovič, Črtomir Podlipnik, Nataša Poklar Ulrih

Food Chemistry, doi:10.1016/j.foodchem.2021.131594

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Conflicts of interest: The authors declare that they have no conflicts of interest. Appendix A. Supplementary material

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