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Evaluation of the Effect of Zinc, Quercetin, Bromelain and Vitamin C on COVID-19 Patients

Ahmed et al., International Journal of Diabetes Management, doi:10.61797/ijdm.v2i2.259, NCT04468139

Oct 2023

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Quercetin for COVID-19

24th treatment shown to reduce risk in July 2021, now with p = 0.0031 from 11 studies.

Lower risk for ICU admission, hospitalization, recovery, cases, and viral clearance.

No treatment is 100% effective. Protocols combine treatments.

5,100+ studies for 109 treatments. c19early.org

Case series of 22 hospitalized COVID-19 patients showing that a combination of quercetin 800mg, bromelain 165mg, zinc acetate 50mg and vitamin C 1g daily for 3-5 days was safe when given with other treatments.

Authors include In Silico molecular docking analysis showing that the natural compounds quercetin and bromelain bind strongly to the active site of human cathepsin L protein, indicating they may act as inhibitors. Quercetin exhibited a binding energy of -5.15 kcal/mol with 5 hydrogen bonds and 1 pi-interaction. Bromelain had a binding energy of -5.65 kcal/mol with 7 hydrogen bonds. As human cathepsin L is involved in inflammatory pathways, the results suggest quercetin and bromelain could potentially help prevent COVID-19 progression to severe inflammation and cytokine storm.

Bioavailability. Quercetin has low bioavailability and studies typically use advanced formulations to improve bioavailability which may be required to reach therapeutic concentrations.

68 preclinical studies support the efficacy of quercetin for COVID-19:

42 In Silico studies1-42

20 In Vitro studies2,12,16,18,22,39,43-56

6 In Vivo animal studies2,16,49,52,57,58

In Silico studies predict inhibition of SARS-CoV-2, or minimization of side effects, with quercetin or metabolites via binding to the spikeA,6,7,19,21,22,27,35,36,38,39,59,60, M^proB,4,6,8,10,12,14,15,17,20,21,27,31,33-35,39,40,42,60,61, RNA-dependent RNA polymeraseC,6,29, PLproD,34,42, ACE2E,19,20,25,34,38,60, TMPRSS2F,19, helicaseG,26,31, endoribonucleaseH,36, NSP16/10I,3, cathepsin LJ,23, Wnt-3K,19, FZDL,19, LRP6M,19, ezrinN,37, ADRPO,35, NRP1P,38, EP300Q,13, PTGS2R,20, HSP90AA1S,13,20, matrix metalloproteinase 9T,28, IL-6U,18,32, IL-10V,18, VEGFAW,32, and RELAX,32 proteins. In Vitro studies demonstrate inhibition of the M^proB,12,43,48,56 protein, and inhibition of spike-ACE2 interactionY,44. In Vitro studies demonstrate efficacy in Calu-3Z,47, A549AA,18, HEK293-ACE2+AB,55, Huh-7AC,22, Caco-2AD,46, Vero E6AE,16,39,46, mTECAF,49, and RAW264.7AG,49 cells. Animal studies demonstrate efficacy in K18-hACE2 miceAH,52, db/db miceAI,49,58, BALB/c miceAJ,57, and rats62. Quercetin reduced proinflammatory cytokines and protected lung and kidney tissue against LPS-induced damage in mice57, inhibits LPS-induced cytokine storm by modulating key inflammatory and antioxidant pathways in macrophages2, and inhibits SARS-CoV-2 ORF3a ion channel activity, which contributes to viral pathogenicity and cytotoxicity51.

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Study covers quercetin, vitamin C, and zinc.

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32. Yang (B) et al., In silico evidence implicating novel mechanisms of Prunella vulgaris L. as a potential botanical drug against COVID-19-associated acute kidney injury, Frontiers in Pharmacology, doi:10.3389/fphar.2023.1188086.frontiersin.org, doi.org.

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41. Sekiou et al., In-Silico Identification of Potent Inhibitors of COVID-19 Main Protease (Mpro) and Angiotensin Converting Enzyme 2 (ACE2) from Natural Products: Quercetin, Hispidulin, and Cirsimaritin Exhibited Better Potential Inhibition than Hydroxy-Chloroquine Against COVID-19 Main Protease Active Site and ACE2, ChemRxiv, doi:10.26434/chemrxiv.12181404.v1.chemrxiv.org, doi.org.

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45. Fang et al., Development of nanoparticles incorporated with quercetin and ACE2-membrane as a novel therapy for COVID-19, Journal of Nanobiotechnology, doi:10.1186/s12951-024-02435-2.biomedcentral.com, doi.org.

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61. Sekiou (B) et al., In-Silico Identification of Potent Inhibitors of COVID-19 Main Protease (Mpro) and Angiotensin Converting Enzyme 2 (ACE2) from Natural Products: Quercetin, Hispidulin, and Cirsimaritin Exhibited Better Potential Inhibition than Hydroxy-Chloroquine Against COVID-19 Main Protease Active Site and ACE2, ChemRxiv, doi:10.26434/chemrxiv.12181404.v1.chemrxiv.org, doi.org.

62. El-Megharbel (B) et al., Chemical and spectroscopic characterization of (Artemisinin/Quercetin/ Zinc) novel mixed ligand complex with assessment of its potent high antiviral activity against SARS-CoV-2 and antioxidant capacity against toxicity induced by acrylamide in male rats, PeerJ, doi:10.7717/peerj.15638.peerj.com, doi.org.

a. The trimeric spike (S) protein is a glycoprotein that mediates viral entry by binding to the host ACE2 receptor, is critical for SARS-CoV-2's ability to infect host cells, and is a target of neutralizing antibodies. Inhibition of the spike protein prevents viral attachment, halting infection at the earliest stage.

b. The main protease or M^pro, also known as 3CL^pro or nsp5, is a cysteine protease that cleaves viral polyproteins into functional units needed for replication. Inhibiting M^pro disrupts the SARS-CoV-2 lifecycle within the host cell, preventing the creation of new copies.

c. RNA-dependent RNA polymerase (RdRp), also called nsp12, is the core enzyme of the viral replicase-transcriptase complex that copies the positive-sense viral RNA genome into negative-sense templates for progeny RNA synthesis. Inhibiting RdRp blocks viral genome replication and transcription.

d. The papain-like protease (PLpro) has multiple functions including cleaving viral polyproteins and suppressing the host immune response by deubiquitination and deISGylation of host proteins. Inhibiting PLpro may block viral replication and help restore normal immune responses.

e. The angiotensin converting enzyme 2 (ACE2) protein is a host cell transmembrane protein that serves as the cellular receptor for the SARS-CoV-2 spike protein. ACE2 is expressed on many cell types, including epithelial cells in the lungs, and allows the virus to enter and infect host cells. Inhibition may affect ACE2's physiological function in blood pressure control.

f. Transmembrane protease serine 2 (TMPRSS2) is a host cell protease that primes the spike protein, facilitating cellular entry. TMPRSS2 activity helps enable cleavage of the spike protein required for membrane fusion and virus entry. Inhibition may especially protect respiratory epithelial cells, buy may have physiological effects.

g. The helicase, or nsp13, protein unwinds the double-stranded viral RNA, a crucial step in replication and transcription. Inhibition may prevent viral genome replication and the creation of new virus components.

h. The endoribonuclease, also known as NendoU or nsp15, cleaves specific sequences in viral RNA which may help the virus evade detection by the host immune system. Inhibition may hinder the virus's ability to mask itself from the immune system, facilitating a stronger immune response.

i. The NSP16/10 complex consists of non-structural proteins 16 and 10, forming a 2'-O-methyltransferase that modifies the viral RNA cap structure. This modification helps the virus evade host immune detection by mimicking host mRNA, making NSP16/10 a promising antiviral target.

j. Cathepsin L is a host lysosomal cysteine protease that can prime the spike protein through an alternative pathway when TMPRSS2 is unavailable. Dual targeting of cathepsin L and TMPRSS2 may maximize disruption of alternative pathways for virus entry.

l. The frizzled (FZD) receptor is a host transmembrane receptor that binds Wnt ligands, initiating the Wnt signaling cascade. FZD serves as a co-receptor, along with ACE2, in some proposed mechanisms of SARS-CoV-2 infection. The virus may take advantage of this pathway as an alternative entry route.

m. Low-density lipoprotein receptor-related protein 6 is a cell surface co-receptor essential for Wnt signaling. LRP6 acts in tandem with FZD for signal transduction and has been discussed as a potential co-receptor for SARS-CoV-2 entry.

n. The ezrin protein links the cell membrane to the cytoskeleton (the cell's internal support structure) and plays a role in cell shape, movement, adhesion, and signaling. Drugs that occupy the same spot on ezrin where the viral spike protein would bind may hindering viral attachment, and drug binding could further stabilize ezrin, strengthening its potential natural capacity to impede viral fusion and entry.

o. The Adipocyte Differentiation-Related Protein (ADRP, also known as Perilipin 2 or PLIN2) is a lipid droplet protein regulating the storage and breakdown of fats in cells. SARS-CoV-2 may hijack the lipid handling machinery of host cells and ADRP may play a role in this process. Disrupting ADRP's interaction with the virus may hinder the virus's ability to use lipids for replication and assembly.

p. Neuropilin-1 (NRP1) is a cell surface receptor with roles in blood vessel development, nerve cell guidance, and immune responses. NRP1 may function as a co-receptor for SARS-CoV-2, facilitating viral entry into cells. Blocking NRP1 may disrupt an alternative route of viral entry.

q. EP300 (E1A Binding Protein P300) is a transcriptional coactivator involved in several cellular processes, including growth, differentiation, and apoptosis, through its acetyltransferase activity that modifies histones and non-histone proteins. EP300 facilitates viral entry into cells and upregulates inflammatory cytokine production.

r. Prostaglandin G/H synthase 2 (PTGS2, also known as COX-2) is an enzyme crucial for the production of inflammatory molecules called prostaglandins. PTGS2 plays a role in the inflammatory response that can become severe in COVID-19 and inhibitors (like some NSAIDs) may have benefits in dampening harmful inflammation, but note that prostaglandins have diverse physiological functions.

s. Heat Shock Protein 90 Alpha Family Class A Member 1 (HSP90AA1) is a chaperone protein that helps other proteins fold correctly and maintains their stability. HSP90AA1 plays roles in cell signaling, survival, and immune responses. HSP90AA1 may interact with numerous viral proteins, but note that it has diverse physiological functions.

t. Matrix metalloproteinase 9 (MMP9), also called gelatinase B, is a zinc-dependent enzyme that breaks down collagen and other components of the extracellular matrix. MMP9 levels increase in severe COVID-19. Overactive MMP9 can damage lung tissue and worsen inflammation. Inhibition of MMP9 may prevent excessive tissue damage and help regulate the inflammatory response.

u. The interleukin-6 (IL-6) pro-inflammatory cytokine (signaling molecule) has a complex role in the immune response and may trigger and perpetuate inflammation. Elevated IL-6 levels are associated with severe COVID-19 cases and cytokine storm. Anti-IL-6 therapies may be beneficial in reducing excessive inflammation in severe COVID-19 cases.

v. The interleukin-10 (IL-10) anti-inflammatory cytokine helps regulate and dampen immune responses, preventing excessive inflammation. IL-10 levels can also be elevated in severe COVID-19. IL-10 could either help control harmful inflammation or potentially contribute to immune suppression.

w. Vascular Endothelial Growth Factor A (VEGFA) promotes the growth of new blood vessels (angiogenesis) and has roles in inflammation and immune responses. VEGFA may contribute to blood vessel leakiness and excessive inflammation associated with severe COVID-19.

x. RELA is a transcription factor subunit of NF-kB and is a key regulator of inflammation, driving pro-inflammatory gene expression. SARS-CoV-2 may hijack and modulate NF-kB pathways.

y. The interaction between the SARS-CoV-2 spike protein and the human ACE2 receptor is a primary method of viral entry, inhibiting this interaction can prevent the virus from attaching to and entering host cells, halting infection at an early stage.

z. Calu-3 is a human lung adenocarcinoma cell line with moderate ACE2 and TMPRSS2 expression and SARS-CoV-2 susceptibility. It provides a model of the human respiratory epithelium, but many not be ideal for modeling early stages of infection due to the moderate expression levels of ACE2 and TMPRSS2.

aa. A549 is a human lung carcinoma cell line with low ACE2 expression and SARS-CoV-2 susceptibility. Viral entry/replication can be studied but the cells may not replicate all aspects of lung infection.

ab. HEK293-ACE2+ is a human embryonic kidney cell line engineered for high ACE2 expression and SARS-CoV-2 susceptibility.

ac. Huh-7 cells were derived from a liver tumor (hepatoma).

ad. Caco-2 cells come from a colorectal adenocarcinoma (cancer). They are valued for their ability to form a polarized cell layer with properties similar to the intestinal lining.

ae. Vero E6 is an African green monkey kidney cell line with low/no ACE2 expression and high SARS-CoV-2 susceptibility. The cell line is easy to maintain and supports robust viral replication, however the monkey origin may not accurately represent human responses.

af. mTEC is a mouse tubular epithelial cell line.

ag. RAW264.7 is a mouse macrophage cell line.

ah. A mouse model expressing the human ACE2 receptor under the control of the K18 promoter.

ai. A mouse model of obesity and severe insulin resistance leading to type 2 diabetes due to a mutation in the leptin receptor gene that impairs satiety signaling.

aj. A mouse model commonly used in infectious disease and cancer research due to higher immune response and susceptibility to infection.

Ahmed et al., 6 Oct 2023, Saudi Arabia, peer-reviewed, mean age 49.3, 6 authors, study period June 2020 - September 2020, trial NCT04468139 (history). Contact: drmedahmed@gmail.com.

This Paper Quercetin All

{ 'indexed': { 'date-parts': [[2023, 12, 31]], 'date-time': '2023-12-31T00:28:40Z', 'timestamp': 1703982520565}, 'reference-count': 0, 'publisher': None, 'issue': '2', 'content-domain': {'domain': [], 'crossmark-restriction': False}, 'abstract': 'Coronavirus disease 2019 (COVID-19) is an infectious disease caused by a new strain ' 'of coronavirus. There are three phases of COVID-19: early infection stage, pulmonary stage ' 'and hyper-inflammation stage respectively. It is important to prevent lung or other organs ' 'injuries by preventing phase-II and phase-III via pharmacological or non-pharmacological ' 'treatments. This was a case series study done on twenty-two patients confirmed to be infected ' 'with SARS-CoV-2 and diagnosed with COVID-19. Patients in this study have been used quercetin ' '800 mg, bromelain 165 mg, zinc acetate 50 mg and ascorbic acid 1 g once daily as supplements ' 'for 3 to 5 days during SARS-CoV-2 infection. The aim of this study is to evaluate the safety ' 'and efficacy of quercetin, bromelain, zinc and ascorbic acid combination supplements on ' 'patients with COVID-19. The mean levels of WBC, ANC, ALC, AMC and AST were normal among all ' 'included patients before and after taking quercetin, bromelain, zinc and ascorbic acid ' 'supplements (P-value >0.05). Quercetin 800 mg, bromelain 165 mg, zinc acetate 50 mg and ' 'ascorbic acid 1 g once daily supplements were safe for patients infected with SARS-CoV-2 and ' 'may prevent poor prognosis. Randomized clinical trials needed in the future to ensure the ' 'efficacy of quercetin, bromelain, zinc and vitamin C combination.', 'DOI': '10.61797/ijdm.v2i2.259', 'type': 'journal-article', 'created': { 'date-parts': [[2023, 12, 30]], 'date-time': '2023-12-30T14:56:19Z', 'timestamp': 1703948179000}, 'page': '29-37', 'source': 'Crossref', 'is-referenced-by-count': 0, 'title': 'Evaluation of the Effect of Zinc, Quercetin, Bromelain and Vitamin C on COVID-19 Patients', 'prefix': '10.61797', 'volume': '2', 'author': [ {'given': 'Amr', 'family': 'Ahmed', 'sequence': 'first', 'affiliation': []}, {'given': 'Heba', 'family': 'Abdelseed', 'sequence': 'additional', 'affiliation': []}, {'given': 'Hassan', 'family': 'Shora', 'sequence': 'additional', 'affiliation': []}, {'given': 'Yousef', 'family': 'Albalawi', 'sequence': 'additional', 'affiliation': []}, {'given': 'Eman', 'family': 'Aslsalameen', 'sequence': 'additional', 'affiliation': []}, {'given': 'Abdullah', 'family': 'Alkattan', 'sequence': 'additional', 'affiliation': []}], 'member': None, 'published-online': {'date-parts': [[2023, 12, 30]]}, 'container-title': 'International Journal of Diabetes Management', 'original-title': [], 'deposited': { 'date-parts': [[2023, 12, 30]], 'date-time': '2023-12-30T14:56:19Z', 'timestamp': 1703948179000}, 'score': 1, 'resource': {'primary': {'URL': 'https://researchlakejournals.com/index.php/IJDM/article/view/259'}}, 'subtitle': [], 'short-title': [], 'issued': {'date-parts': [[2023, 12, 30]]}, 'references-count': 0, 'journal-issue': {'issue': '2', 'published-online': {'date-parts': [[2023, 12, 30]]}}, 'URL': 'http://dx.doi.org/10.61797/ijdm.v2i2.259', 'relation': {}, 'ISSN': ['2564-324X'], 'container-title-short': 'Int J Diabetes Manag', 'published': {'date-parts': [[2023, 12, 30]]}}

Late treatment
is less effective

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