Abstract: Journal Pre-proof Treatment with Quercetin inhibits SARS-CoV-2 N protein-induced acute kidney injury by blocking Smad3-dependent G1 cell cycle arrest Wenjing Wu, Wenbiao Wang, Liying Liang, Junzhe Chen, Biao Wei, Xiao-Ru Huang, Xiaoqin Wang, Xueqing Yu, Hui-Yao Lan PII: S1525-0016(22)00706-7 DOI: https://doi.org/10.1016/j.ymthe.2022.12.002 Reference: YMTHE 5967 To appear in: Molecular Therapy Received Date: 10 October 2022 Accepted Date: 8 December 2022 Please cite this article as: Wu W, Wang W, Liang L, Chen J, Wei B, Huang X-R, Wang X, Yu X, Lan H-Y, Treatment with Quercetin inhibits SARS-CoV-2 N protein-induced acute kidney injury by blocking Smad3-dependent G1 cell cycle arrest, Molecular Therapy (2023), doi: https://doi.org/10.1016/ j.ymthe.2022.12.002. This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. © 2022 The American Society of Gene and Cell Therapy. Jo na ur re lP ro -p of 1 Treatment with Quercetin inhibits SARS-CoV-2 N protein-induced acute kidney injury 2 by blocking Smad3-dependent G1 cell cycle arrest 3 Wenjing Wu1,2,3,4, Wenbiao Wang1, Liying Liang2, Junzhe Chen2,5, Biao Wei2, Xiao-Ru 4 Huang1,2, Xiaoqin Wang3,4, Xueqing Yu1, and Hui-Yao Lan1,2 5 1. Guangdong-Hong Kong Joint Laboratory for Immunological and Genetic Kidney 6 Disease, Departments of Nephrology and Pathology, Guangdong Provincial People’s 7 Hospital, Guangdong Academy of Medical Science, Guangzhou, China. 2. Departments of Medicine & Therapeutics, Li Ka Shing Institute of Health Sciences, Lui 9 Che Woo Institute of Innovative Medicine, and The Chinese University of Hong Kong 10 (CUHK) - Guangdong Academy of Medical Sciences / Guangdong Provincial People’s 11 Hospital (GAMS/GPPH) Joint Research Laboratory on Immunological and Genetic 12 Kidney Diseases, The Chinese University of Hong Kong, Hong Kong, China. re -p r oo f 8 3. Hubei University of Chinese Medicine, Wuhan, China. 14 4. Department of Nephrology, Hubei Provincial Hospital of Traditional Chinese Medicine, 16 17 Hubei Province Academy of Traditional Chinese Medicine, Wuhan, China. ur na 15 lP 13 5. Department of Nephrology, The Third Affiliated hospital, Southern Medical University, Guangzhou, China. 18 Correspondence: Professor Hui-Yao Lan, Departments of Medicine & Therapeutics, Li Ka 20 Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong, China. 21 E-mail: hylan@cuhk.edu.hk; Professor Xueqing Yu, Guangdong Academy of Medical Science, 22 Guangdong 23 yuxueqing@gdph.org.cn; and Professor Xiaoqin Wang, Department of Nephrology, Hubei 24 Provincial Hospital of Traditional Chinese Medicine, Hubei Province Academy of Traditional 25 Chinese Medicine, Wuhan, China, E-mail: wangxiao773@hotmail.com Jo 19 Provincial People’s Hospital, 26 27 28 29 1 Guangzhou, China, E-mail ： 30 31 32 Abstract Increasing evidence shows that SARS-CoV-2 can infect kidneys and cause acute kidney 34 injury..