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Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory effects against COVID-19

Xu et al., Proceedings of the National Academy of Sciences, doi:10.1073/pnas.2301775120
Apr 2023  
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Quercetin for COVID-19
24th treatment shown to reduce risk in July 2021, now with p = 0.002 from 12 studies.
Lower risk for ICU, hospitalization, recovery, cases, and viral clearance.
No treatment is 100% effective. Protocols combine treatments.
5,100+ studies for 112 treatments. c19early.org
In Vitro study of compounds from Huashi Baidu (Q-14), showing dose-dependent inhibition of SARS-CoV-2 with quercetin. Authors also perform a mouse study showing that Q-14 decreases SARS-CoV-2 viral load and reduces pulmonary inflammation.
Among 60 compounds, six (magnolol, glycyrrhisoflavone, licoisoflavone A, emodin, echinatin, and quercetin) showed dose-dependent inhibition of SARS-CoV-2, including two Mpro inhibitors (echinatin and quercetin) and two RdRp inhibitors (glycyrrhisoflavone and licoisoflavone A).
Followup studies1,2 collectively show that quercetin and echinatin had only weak SARS-CoV-2 protease inhibition in SDS-PAGE assays2, but false positive FRET results from MCA-AVLQ quenching1,2. Authors note that the compounds may act via other targets to achieve reported anti-COVID-19 effects2, and underscore the importance of meticulous validation with multiple assays when identifying SARS-CoV-2 protease inhibitors1,2.
Bioavailability. Quercetin has low bioavailability and studies typically use advanced formulations to improve bioavailability which may be required to reach therapeutic concentrations.
73 preclinical studies support the efficacy of quercetin for COVID-19:
45 In Silico studies3-47
22 In Vitro studies3,4,7,17,21,23,27,44,48-61
6 In Vivo animal studies7,21,54,57,62,63
In Silico studies predict inhibition of SARS-CoV-2, or minimization of side effects, with quercetin or metabolites via binding to the spikeA,5,11,12,24,26,27,32,40,41,43,44,64-66, MproB,5,9,11,13,15,17,19,20,22,25,26,32,36,38-40,44,45,47,65-67, RNA-dependent RNA polymeraseC,3,5,11,34,66, PLproD,5,39,47, ACE2E,24,25,30,39,43,65, TMPRSS2F,24, nucleocapsidG,5, helicaseH,5,31,36, endoribonucleaseI,41, NSP16/10J,8, cathepsin LK,28, Wnt-3L,24, FZDM,24, LRP6N,24, ezrinO,42, ADRPP,40, NRP1Q,43, EP300R,18, PTGS2S,25, HSP90AA1T,18,25, matrix metalloproteinase 9U,33, IL-6V,23,37, IL-10W,23, VEGFAX,37, and RELAY,37 proteins. In Vitro studies demonstrate inhibition of the MproB,17,48,53,61 protein, and inhibition of spike-ACE2 interactionZ,49. In Vitro studies demonstrate efficacy in Calu-3AA,52, A549AB,23, HEK293-ACE2+AC,60, Huh-7AD,27, Caco-2AE,51, Vero E6AF,21,44,51, mTECAG,54, and RAW264.7AH,54 cells. Animal studies demonstrate efficacy in K18-hACE2 miceAI,57, db/db miceAJ,54,63, BALB/c miceAK,62, and rats68. Quercetin reduced proinflammatory cytokines and protected lung and kidney tissue against LPS-induced damage in mice62, inhibits LPS-induced cytokine storm by modulating key inflammatory and antioxidant pathways in macrophages7, and inhibits SARS-CoV-2 ORF3a ion channel activity, which contributes to viral pathogenicity and cytotoxicity56.
Important missing comments or errors? Let us know.
a. The trimeric spike (S) protein is a glycoprotein that mediates viral entry by binding to the host ACE2 receptor, is critical for SARS-CoV-2's ability to infect host cells, and is a target of neutralizing antibodies. Inhibition of the spike protein prevents viral attachment, halting infection at the earliest stage.
b. The main protease or Mpro, also known as 3CLpro or nsp5, is a cysteine protease that cleaves viral polyproteins into functional units needed for replication. Inhibiting Mpro disrupts the SARS-CoV-2 lifecycle within the host cell, preventing the creation of new copies.
c. RNA-dependent RNA polymerase (RdRp), also called nsp12, is the core enzyme of the viral replicase-transcriptase complex that copies the positive-sense viral RNA genome into negative-sense templates for progeny RNA synthesis. Inhibiting RdRp blocks viral genome replication and transcription.
d. The papain-like protease (PLpro) has multiple functions including cleaving viral polyproteins and suppressing the host immune response by deubiquitination and deISGylation of host proteins. Inhibiting PLpro may block viral replication and help restore normal immune responses.
e. The angiotensin converting enzyme 2 (ACE2) protein is a host cell transmembrane protein that serves as the cellular receptor for the SARS-CoV-2 spike protein. ACE2 is expressed on many cell types, including epithelial cells in the lungs, and allows the virus to enter and infect host cells. Inhibition may affect ACE2's physiological function in blood pressure control.
f. Transmembrane protease serine 2 (TMPRSS2) is a host cell protease that primes the spike protein, facilitating cellular entry. TMPRSS2 activity helps enable cleavage of the spike protein required for membrane fusion and virus entry. Inhibition may especially protect respiratory epithelial cells, buy may have physiological effects.
g. The nucleocapsid (N) protein binds and encapsulates the viral genome by coating the viral RNA. N enables formation and release of infectious virions and plays additional roles in viral replication and pathogenesis. N is also an immunodominant antigen used in diagnostic assays.
h. The helicase, or nsp13, protein unwinds the double-stranded viral RNA, a crucial step in replication and transcription. Inhibition may prevent viral genome replication and the creation of new virus components.
i. The endoribonuclease, also known as NendoU or nsp15, cleaves specific sequences in viral RNA which may help the virus evade detection by the host immune system. Inhibition may hinder the virus's ability to mask itself from the immune system, facilitating a stronger immune response.
j. The NSP16/10 complex consists of non-structural proteins 16 and 10, forming a 2'-O-methyltransferase that modifies the viral RNA cap structure. This modification helps the virus evade host immune detection by mimicking host mRNA, making NSP16/10 a promising antiviral target.
k. Cathepsin L is a host lysosomal cysteine protease that can prime the spike protein through an alternative pathway when TMPRSS2 is unavailable. Dual targeting of cathepsin L and TMPRSS2 may maximize disruption of alternative pathways for virus entry.
l. Wingless-related integration site (Wnt) ligand 3 is a host signaling molecule that activates the Wnt signaling pathway, which is important in development, cell growth, and tissue repair. Some studies suggest that SARS-CoV-2 infection may interfere with the Wnt signaling pathway, and that Wnt3a is involved in SARS-CoV-2 entry.
m. The frizzled (FZD) receptor is a host transmembrane receptor that binds Wnt ligands, initiating the Wnt signaling cascade. FZD serves as a co-receptor, along with ACE2, in some proposed mechanisms of SARS-CoV-2 infection. The virus may take advantage of this pathway as an alternative entry route.
n. Low-density lipoprotein receptor-related protein 6 is a cell surface co-receptor essential for Wnt signaling. LRP6 acts in tandem with FZD for signal transduction and has been discussed as a potential co-receptor for SARS-CoV-2 entry.
o. The ezrin protein links the cell membrane to the cytoskeleton (the cell's internal support structure) and plays a role in cell shape, movement, adhesion, and signaling. Drugs that occupy the same spot on ezrin where the viral spike protein would bind may hindering viral attachment, and drug binding could further stabilize ezrin, strengthening its potential natural capacity to impede viral fusion and entry.
p. The Adipocyte Differentiation-Related Protein (ADRP, also known as Perilipin 2 or PLIN2) is a lipid droplet protein regulating the storage and breakdown of fats in cells. SARS-CoV-2 may hijack the lipid handling machinery of host cells and ADRP may play a role in this process. Disrupting ADRP's interaction with the virus may hinder the virus's ability to use lipids for replication and assembly.
q. Neuropilin-1 (NRP1) is a cell surface receptor with roles in blood vessel development, nerve cell guidance, and immune responses. NRP1 may function as a co-receptor for SARS-CoV-2, facilitating viral entry into cells. Blocking NRP1 may disrupt an alternative route of viral entry.
r. EP300 (E1A Binding Protein P300) is a transcriptional coactivator involved in several cellular processes, including growth, differentiation, and apoptosis, through its acetyltransferase activity that modifies histones and non-histone proteins. EP300 facilitates viral entry into cells and upregulates inflammatory cytokine production.
s. Prostaglandin G/H synthase 2 (PTGS2, also known as COX-2) is an enzyme crucial for the production of inflammatory molecules called prostaglandins. PTGS2 plays a role in the inflammatory response that can become severe in COVID-19 and inhibitors (like some NSAIDs) may have benefits in dampening harmful inflammation, but note that prostaglandins have diverse physiological functions.
t. Heat Shock Protein 90 Alpha Family Class A Member 1 (HSP90AA1) is a chaperone protein that helps other proteins fold correctly and maintains their stability. HSP90AA1 plays roles in cell signaling, survival, and immune responses. HSP90AA1 may interact with numerous viral proteins, but note that it has diverse physiological functions.
u. Matrix metalloproteinase 9 (MMP9), also called gelatinase B, is a zinc-dependent enzyme that breaks down collagen and other components of the extracellular matrix. MMP9 levels increase in severe COVID-19. Overactive MMP9 can damage lung tissue and worsen inflammation. Inhibition of MMP9 may prevent excessive tissue damage and help regulate the inflammatory response.
v. The interleukin-6 (IL-6) pro-inflammatory cytokine (signaling molecule) has a complex role in the immune response and may trigger and perpetuate inflammation. Elevated IL-6 levels are associated with severe COVID-19 cases and cytokine storm. Anti-IL-6 therapies may be beneficial in reducing excessive inflammation in severe COVID-19 cases.
w. The interleukin-10 (IL-10) anti-inflammatory cytokine helps regulate and dampen immune responses, preventing excessive inflammation. IL-10 levels can also be elevated in severe COVID-19. IL-10 could either help control harmful inflammation or potentially contribute to immune suppression.
x. Vascular Endothelial Growth Factor A (VEGFA) promotes the growth of new blood vessels (angiogenesis) and has roles in inflammation and immune responses. VEGFA may contribute to blood vessel leakiness and excessive inflammation associated with severe COVID-19.
y. RELA is a transcription factor subunit of NF-kB and is a key regulator of inflammation, driving pro-inflammatory gene expression. SARS-CoV-2 may hijack and modulate NF-kB pathways.
z. The interaction between the SARS-CoV-2 spike protein and the human ACE2 receptor is a primary method of viral entry, inhibiting this interaction can prevent the virus from attaching to and entering host cells, halting infection at an early stage.
aa. Calu-3 is a human lung adenocarcinoma cell line with moderate ACE2 and TMPRSS2 expression and SARS-CoV-2 susceptibility. It provides a model of the human respiratory epithelium, but many not be ideal for modeling early stages of infection due to the moderate expression levels of ACE2 and TMPRSS2.
ab. A549 is a human lung carcinoma cell line with low ACE2 expression and SARS-CoV-2 susceptibility. Viral entry/replication can be studied but the cells may not replicate all aspects of lung infection.
ac. HEK293-ACE2+ is a human embryonic kidney cell line engineered for high ACE2 expression and SARS-CoV-2 susceptibility.
ad. Huh-7 cells were derived from a liver tumor (hepatoma).
ae. Caco-2 cells come from a colorectal adenocarcinoma (cancer). They are valued for their ability to form a polarized cell layer with properties similar to the intestinal lining.
af. Vero E6 is an African green monkey kidney cell line with low/no ACE2 expression and high SARS-CoV-2 susceptibility. The cell line is easy to maintain and supports robust viral replication, however the monkey origin may not accurately represent human responses.
ag. mTEC is a mouse tubular epithelial cell line.
ah. RAW264.7 is a mouse macrophage cell line.
ai. A mouse model expressing the human ACE2 receptor under the control of the K18 promoter.
aj. A mouse model of obesity and severe insulin resistance leading to type 2 diabetes due to a mutation in the leptin receptor gene that impairs satiety signaling.
ak. A mouse model commonly used in infectious disease and cancer research due to higher immune response and susceptibility to infection.
Xu et al., 24 Apr 2023, China, peer-reviewed, 33 authors. Contact: gaof@im.ac.cn, huangluqi01@126.com.
In Vitro studies are an important part of preclinical research, however results may be very different in vivo.
This PaperQuercetinAll
Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory effects against COVID-19
Haiyu Xu, Shufen Li, Jiayuan Liu, Jinlong Cheng, Liping Kang, Weijie Li, Yute Zhong, Chaofa Wei, Lifeng Fu, Jianxun Qi, Yulan Zhang, Miaomiao You, Zhenxing Zhou, Chongtao Zhang, Haixia Su, Sheng Yao, Zhaoyin Zhou, Yulong Shi, Ran Deng, Qi Lv, Fengdi Li, Feifei Qi, Jie Chen, Siqin Zhang, Xiaojing Ma, Zhijian Xu, Shao Li, Yechun Xu, Ke Peng, Yi Shi, Hualiang Jiang, George F Gao, Luqi Huang
Proceedings of the National Academy of Sciences, doi:10.1073/pnas.2301775120
Significance Huashi Baidu decoction (Q-14), a famous traditional Chinese medicine decoction for COVID-19, has good effects on SARS-CoV-2 RNA clearance, promoting lung lesion opacity absorption, reducing inflammation, and ameliorating flu-like symptoms based on a series of clinical trials, having potent antiviral and antiinflammatory effects. However, due to the lack of systematic and in-depth research, little significant progress has been made in the study of TCMT-NDRD of HBF for COVID-19 management. Therefore, the aim of the current study was to identify key antiviral and anti-inflammatory bioactive compounds from HBF based on an integrative pharmacological strategy. These findings will greatly stimulate the traditional Chinese medicine theory-driven natural drug discovery against COVID-19 and promote the modern research of Chinese medicine.
References
Adams, Grosse-Kunstleve, Li, Ioerger, Terwilliger, PHENIX: Building new software for automated crystallographic structure determination, Acta Crystallogr. D
Al-Kuraishy, Sequential doxycycline and colchicine combination therapy in Covid-19: The salutary effects, Pulm. Pharmacol. Ther
Baell, Walters, Chemistry: Chemical con artists foil drug discovery, Nature
Bai, Structural basis for the inhibition of the SARS-CoV-2 main protease by the anti-HCV drug narlaprevir, Signal Transduct. Targeted Ther
Bailey, The ccp4 suite -programs for protein crystallography, Acta Crystallogr. Sect. D
Bao, The pathogenicity of SARS-CoV-2 in hACE2 transgenic mice, Nature, doi:10.1073/pnas.230177512011of11
Bernal, Molnupiravir for oral treatment of Covid-19 in nonhospitalized patients, N. Engl. J. Med
Chen, 5-hydroxymethylcytosine signatures in circulating cell-free DNA as early warning biomarkers for COVID-19 progression and myocardial injury, Front. Cell Dev. Biol
Emsley, Cowtan, Coot: Model-building tools for molecular graphics, Acta Crystallogr. D
Feng, Li, Zhang, Yin, Li, Crystal structure of SARS-CoV 3C-like protease with baicalein, Biochem. Biophys. Res. Commun
Fu, Mechanism of microbial metabolite leupeptin in the treatment of COVID-19 by traditional Chinese medicine herbs, mBio
Gordon, Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency, J. Biol. Chem
Gottlieb, Effect of bamlanivimab as monotherapy or in combination with etesevimab on viral load in patients with mild to moderate COVID-19: A randomized clinical trial, Jama
Huang, Clinical features of patients infected with 2019 novel coronavirus in Wuhan. China, Lancet
Huang, Traditional Chinese medicine (TCM) in the treatment of COVID-19 and other viral infections: Efficacies and mechanisms, Pharmacol. Therapeut
Ingolfsson, Phytochemicals perturb membranes and promiscuously alter protein function, ACS Chem. Biol
Koes, Baumgartner, Camacho, Lessons learned in empirical scoring with smina from the CSAR 2011 benchmarking exercise, J. Chem. Inf. Model
Li, Potential treatment of COVID-19 with traditional chinese medicine: What herbs can help win the battle with SARS-CoV-2?, Engineering
Li, SARS-CoV-2 Z-RNA activates the ZBP1-RIPK3 pathway to promote virus-induced inflammatory responses, Cell Res
Li, SARS-CoV-2 triggers inflammatory responses and cell death through caspase-8 activation, Signal Transduct. Target. Ther
Li, SFTSV infection induces BAK/BAX-dependent Mitochondrial DNA release to trigger NLRP3 inflammasome activation, Cell Rep
Liu, A novel STING agonist-adjuvanted pan-sarbecovirus vaccine elicits potent and durable neutralizing antibody and T cell responses in mice, rabbits and NHPs, Cell Res
Liu, Combination of Hua Shi Bai Du granule (Q-14) and standard care in the treatment of patients with coronavirus disease 2019 (COVID-19): A single-center, open-label, randomized controlled trial, Phytomedicine
Liu, He, Huang, Wei, Zhu, Severe fever with thrombocytopenia syndrome, an emerging tick-borne zoonosis, Lancet. Infect. Dis
Liu, Herb-drug interactions involving drug metabolizing enzymes and transporters, Curr. Drug. Metab
Liu, SFTSV infection induced interleukin-1β secretion through NLRP3 inflammasome activation, Front. Immunol
Lyu, Traditional Chinese medicine in COVID-19, Acta Pharm. Sin. B
Mangalmurti, Hunter, Cytokine storms: Understanding COVID-19, Immunity
Maurice, Advances in targeting cyclic nucleotide phosphodiesterases, Nat. Rev. Drug. Discov
Minor, Cymborowski, Otwinowski, Chruszcz, Hkl-, The integration of data reduction and structure solution-from diffraction images to an initial model in minutes, Acta Crystallogr. D
Najjar-Debbiny, Effectiveness of paxlovid in reducing severe COVID-19 and mortality in high risk patients, Clin. Infect. Dis
Niu, Network pharmacology analysis to identify phytochemicals in traditional Chinese medicines that may regulate ACE2 for the treatment of COVID-19, Evid. Based Complement. Alternat. Med
Niu, Network pharmacology for the identification of phytochemicals in traditional Chinese medicine for COVID-19 that may regulate interleukin-6, Biosci. Rep
Peng, Structural and biochemical characterization of the nsp12-nsp7-nsp8 core polymerase complex from SARS-CoV-2, Cell Rep
Runfeng, Lianhuaqingwen exerts anti-viral and anti-inflammatory activity against novel coronavirus (SARS-CoV-2), Pharmacol. Res
Saravolatz, Depcinski, Sharma, Molnupiravir and nirmatrelvir-ritonavir: Oral Coronavirus disease 2019 antiviral drugs, Clin. Infect. Dis
Sen, Identification of potential edible mushroom as SARS-CoV-2 main protease inhibitor using rational drug designing approach, Sci. Rep
Shankar-Hari, Association between administration of IL-6 antagonists and mortality among patients hospitalized for COVID-19: A meta-analysis, Jama
Shi, D3Targets-2019-nCoV: A webserver for predicting drug targets and for multi-target and multi-site based virtual screening against COVID-19, Acta Pharm. Sin. B
Shi, Efficacy and safety of Chinese herbal medicine versus Lopinavir-Ritonavir in adult patients with coronavirus disease 2019: A non-randomized controlled trial, Phytomedicine
Su, Identification of pyrogallol as a warhead in design of covalent inhibitors for the SARS-CoV-2 3CL protease, Nat. Commun
Tan, A novel coronavirus genome identified in a cluster of pneumonia cases -Wuhan, China 2019-2020, China CDC weekly
Trott, Olson, AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading, J. Comput. Chem
Tu, The discovery of artemisinin (qinghaosu) and gifts from Chinese medicine, Nat. Med
Wang, Artemisinin, the magic drug discovered from traditional Chinese medicine, Engineering
Wang, Deciphering the active compounds and mechanisms of HSBDF for treating ALI via integrating chemical bioinformatics analysis, Front. Pharmacol
Wang, Upgrade of macromolecular crystallography beamline BL17U1at SSRF, Nucl Sci. Tech
Wang, Zhang, Zhan, Gao, Screening out anti-inflammatory or anti-viral targets in Xuanfei Baidu Tang through a new technique of reverse finding target, Bioorg. Chem
Wegesser, Coppi, Harper, Paris, Minocherhomji, Nonclinical genotoxicity and carcinogenicity profile of apremilast, an oral selective inhibitor of PDE4, Regul. Toxicol. Pharmacol. RTP
Wei, Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS, Chinese J. Nat Med
Xiong, The effect of Huashibaidu formula on the blood oxygen saturation status of severe COVID-19: A retrospective cohort study, Phytomed. Int. J. Phytothera. Phytopharmacol
Xiong, Wang, Du, Ai, Efficacy of herbal medicine (Xuanfei Baidu decoction) combined with conventional drug in treating COVID-19: A pilot randomized clinical trial, Integra. Med. Res
Xu, A comprehensive review of integrative pharmacology-based investigation: A paradigm shift in traditional Chinese medicine, Acta Pharm. Sin. B
Xu, ETCM: An encyclopaedia of traditional Chinese medicine, Nucleic Acids Res
Yang, Mice transgenic for human angiotensin-converting enzyme 2 provide a model for SARS coronavirus infection, Comparat. Med
Yu, Ju, Ding, A nucleocapsid-based transcomplementation cell culture system of SARS-CoV-2 to recapitulate the complete viral life cycle, Bio Protocol
Zebda, Paller, Phosphodiesterase 4 inhibitors, J. Am. Acad. Dermatol
Zhang, Association between use of Qingfei Paidu Tang and mortality in hospitalized patients with COVID-19: A national retrospective registry study, Phytomedicine
Zhang, Dong, Li, Chen, Xu, Structure-aided identification and optimization of tetrahydro-isoquinolines as novel PDE4 inhibitors leading to discovery of an effective anti-psoriasis agent, J. Med. Chem
Zhang, Ibrahim, Gillette, Bollag, Phosphodiesterase-4 as a potential drug target, Expert. Opin. Ther. Targets
Zhang, Li, Zhang, Li, Liu, Design, synthesis, and biological evaluation of tetrahydroisoquinolines derivatives as novel, selective PDE4 inhibitors for antipsoriasis treatment, Eur. J. Med. Chem
Zhang, SoFDA: An integrated web platform from syndrome ontology to network-based evaluation of disease-syndrome-formula associations for precision medicine, Sci. Bull
Zhao, Chinese medicine formula huashibaidu granule early treatment for mild COVID-19 patients: An unblinded, cluster-randomized clinical trial, Front. Med
Zhou, A pneumonia outbreak associated with a new coronavirus of probable bat origin, Nature
Zhu, A novel coronavirus from patients with pneumonia in China, N. Engl. J. Med
Zhu, Analyzing the potential therapeutic mechanism of Huashi Baidu Decoction on severe COVID-19 through integrating network pharmacological methods, J. Tradit. Complement. Med
Zumla, Hui, Perlman, Middle east respiratory syndrome, Lancet
{ 'indexed': {'date-parts': [[2023, 4, 25]], 'date-time': '2023-04-25T04:35:47Z', 'timestamp': 1682397347666}, 'reference-count': 0, 'publisher': 'Proceedings of the National Academy of Sciences', 'issue': '18', 'license': [ { 'start': { 'date-parts': [[2023, 4, 24]], 'date-time': '2023-04-24T00:00:00Z', 'timestamp': 1682294400000}, 'content-version': 'vor', 'delay-in-days': 0, 'URL': 'https://creativecommons.org/licenses/by-nc-nd/4.0/'}], 'funder': [ { 'DOI': '10.13039/501100001809', 'name': 'National Natural Science Foundation of China', 'doi-asserted-by': 'publisher', 'award': ['81830111']}, { 'name': 'Establishment of Sino-Austria &quot;Belt and Road&quot; Joint Laboratory on ' 'Traditional Chinese Medicine for Severe Infectious Diseases and Joint Research', 'award': ['2020YFE0205100']}, { 'name': 'The ability establishment of sustainable use for valuablee Chinese Medicine ' 'Resources', 'award': ['2060302']}], 'content-domain': {'domain': ['www.pnas.org'], 'crossmark-restriction': True}, 'published-print': {'date-parts': [[2023, 5, 2]]}, 'abstract': '<jats:p>\n' ' The coronavirus disease 2019 (COVID-19) pandemic is an ongoing global health ' 'concern, and effective antiviral reagents are urgently needed. Traditional Chinese medicine ' 'theory-driven natural drug research and development (TCMT-NDRD) is a feasible method to ' 'address this issue as the traditional Chinese medicine formulae have been shown effective in ' 'the treatment of COVID-19. Huashi Baidu decoction (Q-14) is a clinically approved formula for ' 'COVID-19 therapy with antiviral and anti-inflammatory effects. Here, an integrative ' 'pharmacological strategy was applied to identify the antiviral and anti-inflammatory ' 'bioactive compounds from Q-14. Overall, a total of 343 chemical compounds were initially ' 'characterized, and 60 prototype compounds in Q-14 were subsequently traced in plasma using ' 'ultrahigh-performance liquid chromatography with quadrupole time-of-flight mass spectrometry. ' 'Among the 60 compounds, six compounds (magnolol, glycyrrhisoflavone, licoisoflavone A, ' 'emodin, echinatin, and quercetin) were identified showing a dose-dependent inhibition effect ' 'on the SARS-CoV-2 infection, including two inhibitors (echinatin and quercetin) of the main ' 'protease (M\n' ' <jats:sup>pro</jats:sup>\n' ' ), as well as two inhibitors (glycyrrhisoflavone and licoisoflavone A) of the ' 'RNA-dependent RNA polymerase (RdRp). Meanwhile, three anti-inflammatory components, including ' 'licochalcone B, echinatin, and glycyrrhisoflavone, were identified in a SARS-CoV-2-infected ' 'inflammatory cell model. In addition, glycyrrhisoflavone and licoisoflavone A also displayed ' 'strong inhibitory activities against cAMP-specific 3′,5′-cyclic phosphodiesterase 4 (PDE4). ' 'Crystal structures of PDE4 in complex with glycyrrhisoflavone or licoisoflavone A were ' 'determined at resolutions of 1.54\xa0Å and 1.65\xa0Å, respectively, and both compounds bind ' 'in the active site of PDE4 with similar interactions. These findings will greatly stimulate ' 'the study of TCMT-NDRD against COVID-19.\n' ' </jats:p>', 'DOI': '10.1073/pnas.2301775120', 'type': 'journal-article', 'created': {'date-parts': [[2023, 4, 24]], 'date-time': '2023-04-24T19:12:52Z', 'timestamp': 1682363572000}, 'update-policy': 'http://dx.doi.org/10.1073/pnas.cm10313', 'source': 'Crossref', 'is-referenced-by-count': 0, 'title': 'Bioactive compounds from Huashi Baidu decoction possess both antiviral and anti-inflammatory ' 'effects against COVID-19', 'prefix': '10.1073', 'volume': '120', 'author': [ { 'given': 'Haiyu', 'family': 'Xu', 'sequence': 'first', 'affiliation': [ { 'name': 'Institute of Chinese Materia Medica, Academy of Chinese Medical ' 'Sciences, Beijing 100700, China'}]}, { 'given': 'Shufen', 'family': 'Li', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Virology, Center for Antiviral Research, ' 'Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan ' '430207, China'}]}, { 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'Sciences, Beijing 100700, China'}]}, { 'given': 'Yute', 'family': 'Zhong', 'sequence': 'additional', 'affiliation': [ { 'name': 'Institute of Chinese Materia Medica, Academy of Chinese Medical ' 'Sciences, Beijing 100700, China'}]}, { 'given': 'Chaofa', 'family': 'Wei', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Dao-di Herbs, National Resource Center ' 'for Chinese Materia Medica, China Academy of Chinese Medical ' 'Sciences, Beijing 100700, China'}]}, { 'given': 'Lifeng', 'family': 'Fu', 'sequence': 'additional', 'affiliation': [ { 'name': 'Chinese Academy of Sciences (CAS) Key Laboratory of Pathogen ' 'Microbiology and Immunology, Institute of Microbiology, Chinese ' 'Academy of Sciences, Beijing 100101, China'}]}, { 'ORCID': 'http://orcid.org/0000-0002-9358-4732', 'authenticated-orcid': False, 'given': 'Jianxun', 'family': 'Qi', 'sequence': 'additional', 'affiliation': [ { 'name': 'Chinese Academy of Sciences (CAS) Key Laboratory of Pathogen ' 'Microbiology and Immunology, Institute of Microbiology, Chinese ' 'Academy of Sciences, Beijing 100101, China'}, {'name': 'Beijing Life Science Academy, Beijing 102209, China'}]}, { 'given': 'Yulan', 'family': 'Zhang', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Virology, Center for Antiviral Research, ' 'Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan ' '430207, China'}]}, { 'given': 'Miaomiao', 'family': 'You', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Virology, Center for Antiviral Research, ' 'Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan ' '430207, China'}]}, { 'given': 'Zhenxing', 'family': 'Zhou', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Virology, Center for Antiviral Research, ' 'Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan ' '430207, China'}]}, { 'given': 'Chongtao', 'family': 'Zhang', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Virology, Center for Antiviral Research, ' 'Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan ' '430207, China'}]}, { 'given': 'Haixia', 'family': 'Su', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Drug Research, Shanghai Institute of ' 'Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ' 'China'}]}, { 'given': 'Sheng', 'family': 'Yao', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Drug Research, Shanghai Institute of ' 'Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ' 'China'}]}, { 'given': 'Zhaoyin', 'family': 'Zhou', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Drug Research, Shanghai Institute of ' 'Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ' 'China'}]}, { 'given': 'Yulong', 'family': 'Shi', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Drug 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'sequence': 'additional', 'affiliation': [ { 'name': 'Beijing Key Laboratory for Animal Models of Emerging and ' 'Reemerging Infectious Diseases, Key Laboratory of Comparative ' 'Medicine for Human Diseases of the National Health Commission, ' 'Institute of Laboratory Animal Sciences, Chinese Academy of ' 'Medical Sciences &amp; Peking Union Medical College, Beijing ' '100021, China'}]}, { 'given': 'Feifei', 'family': 'Qi', 'sequence': 'additional', 'affiliation': [ { 'name': 'Beijing Key Laboratory for Animal Models of Emerging and ' 'Reemerging Infectious Diseases, Key Laboratory of Comparative ' 'Medicine for Human Diseases of the National Health Commission, ' 'Institute of Laboratory Animal Sciences, Chinese Academy of ' 'Medical Sciences &amp; Peking Union Medical College, Beijing ' '100021, China'}]}, { 'given': 'Jie', 'family': 'Chen', 'sequence': 'additional', 'affiliation': [ { 'name': 'Institute of Chinese Materia Medica, Academy of Chinese Medical ' 'Sciences, Beijing 100700, China'}]}, { 'given': 'Siqin', 'family': 'Zhang', 'sequence': 'additional', 'affiliation': [ { 'name': 'Institute for Traditional Chinese Medicine-X, Ministry of ' 'Education Key Laboratory of Bioinformatics/Bioinformatics ' 'Division, Beijing National Research Center for Information ' 'Science and Technology, Department of Automation, Tsinghua ' 'University, Beijing 100084, China'}]}, { 'given': 'Xiaojing', 'family': 'Ma', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Dao-di Herbs, National Resource Center ' 'for Chinese Materia Medica, China Academy of Chinese Medical ' 'Sciences, Beijing 100700, China'}]}, { 'given': 'Zhijian', 'family': 'Xu', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Drug Research, Shanghai Institute of ' 'Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ' 'China'}]}, { 'given': 'Shao', 'family': 'Li', 'sequence': 'additional', 'affiliation': [ { 'name': 'Institute for Traditional Chinese Medicine-X, Ministry of ' 'Education Key Laboratory of Bioinformatics/Bioinformatics ' 'Division, Beijing National Research Center for Information ' 'Science and Technology, Department of Automation, Tsinghua ' 'University, Beijing 100084, China'}]}, { 'given': 'Yechun', 'family': 'Xu', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Drug Research, Shanghai Institute of ' 'Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ' 'China'}]}, { 'given': 'Ke', 'family': 'Peng', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Virology, Center for Antiviral Research, ' 'Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan ' '430207, China'}]}, { 'given': 'Yi', 'family': 'Shi', 'sequence': 'additional', 'affiliation': [ { 'name': 'Chinese Academy of Sciences (CAS) Key Laboratory of Pathogen ' 'Microbiology and Immunology, Institute of Microbiology, Chinese ' 'Academy of Sciences, Beijing 100101, China'}, {'name': 'Beijing Life Science Academy, Beijing 102209, China'}]}, { 'given': 'Hualiang', 'family': 'Jiang', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Drug Research, Shanghai Institute of ' 'Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ' 'China'}]}, { 'ORCID': 'http://orcid.org/0000-0002-3869-615X', 'authenticated-orcid': False, 'given': 'George F.', 'family': 'Gao', 'sequence': 'additional', 'affiliation': [ { 'name': 'Chinese Academy of Sciences (CAS) Key Laboratory of Pathogen ' 'Microbiology and Immunology, Institute of Microbiology, Chinese ' 'Academy of Sciences, Beijing 100101, China'}, {'name': 'Beijing Life Science Academy, Beijing 102209, China'}]}, { 'given': 'Luqi', 'family': 'Huang', 'sequence': 'additional', 'affiliation': [ { 'name': 'State Key Laboratory of Dao-di Herbs, National Resource Center ' 'for Chinese Materia Medica, China Academy of Chinese Medical ' 'Sciences, Beijing 100700, China'}]}], 'member': '341', 'published-online': {'date-parts': [[2023, 4, 24]]}, 'container-title': 'Proceedings of the National Academy of Sciences', 'original-title': [], 'language': 'en', 'link': [ { 'URL': 'https://pnas.org/doi/pdf/10.1073/pnas.2301775120', 'content-type': 'unspecified', 'content-version': 'vor', 'intended-application': 'similarity-checking'}], 'deposited': { 'date-parts': [[2023, 4, 24]], 'date-time': '2023-04-24T19:15:31Z', 'timestamp': 1682363731000}, 'score': 1, 'resource': {'primary': {'URL': 'https://pnas.org/doi/10.1073/pnas.2301775120'}}, 'subtitle': [], 'short-title': [], 'issued': {'date-parts': [[2023, 4, 24]]}, 'references-count': 0, 'journal-issue': {'issue': '18', 'published-print': {'date-parts': [[2023, 5, 2]]}}, 'alternative-id': ['10.1073/pnas.2301775120'], 'URL': 'http://dx.doi.org/10.1073/pnas.2301775120', 'relation': {}, 'ISSN': ['0027-8424', '1091-6490'], 'subject': ['Multidisciplinary'], 'container-title-short': 'Proc. Natl. Acad. Sci. U.S.A.', 'published': {'date-parts': [[2023, 4, 24]]}, 'assertion': [ { 'value': '2023-02-02', 'order': 0, 'name': 'received', 'label': 'Received', 'group': {'name': 'publication_history', 'label': 'Publication History'}}, { 'value': '2023-03-14', 'order': 1, 'name': 'accepted', 'label': 'Accepted', 'group': {'name': 'publication_history', 'label': 'Publication History'}}, { 'value': '2023-04-24', 'order': 2, 'name': 'published', 'label': 'Published', 'group': {'name': 'publication_history', 'label': 'Publication History'}}]}
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Please send us corrections, updates, or comments. c19early involves the extraction of 100,000+ datapoints from thousands of papers. Community updates help ensure high accuracy. Treatments and other interventions are complementary. All practical, effective, and safe means should be used based on risk/benefit analysis. No treatment or intervention is 100% available and effective for all current and future variants. We do not provide medical advice. Before taking any medication, consult a qualified physician who can provide personalized advice and details of risks and benefits based on your medical history and situation. FLCCC and WCH provide treatment protocols.
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