Analgesics⏵
Antiandrogens⏵
Antihistamines
Azvudine
Bromhexine
Budesonide
Colchicine
Conv. Plasma
Curcumin
Famotidine
Favipiravir
Fluvoxamine
Hydroxychlor..
Ivermectin
Lifestyle⏵
Melatonin
Metformin
Minerals⏵
Molnupiravir
Monoclonals⏵
Naso/orophar..⏵
Nigella Sativa
Nitazoxanide
PPIs
Paxlovid
Quercetin
Remdesivir
Thermotherapy
Vitamins⏵
More⏵

Other
Feedback
Home
 
Top
..
Abstract
..
All quercetin studies
..
Meta analysis
..
Feedback
Home
←
next
study
→
previous
study
c19early.org COVID-19 treatment researchQuercetinQuercetin (more..)
Melatonin Meta
Metformin Meta
Antihistamines Meta
Azvudine Meta Molnupiravir Meta
Bromhexine Meta
Budesonide Meta
Colchicine Meta Nigella Sativa Meta
Conv. Plasma Meta Nitazoxanide Meta
Curcumin Meta PPIs Meta
Famotidine Meta Paxlovid Meta
Favipiravir Meta Quercetin Meta
Fluvoxamine Meta Remdesivir Meta
Hydroxychlor.. Meta Thermotherapy Meta
Ivermectin Meta
Home Adoption Other Treatments
@CovidAnalysis
All Studies   Meta Analysis       

Flavonoids as Potent Inhibitor of SARS-CoV-2 Nsp13 Helicase: Grid Based Docking Approach

Singh et al., Middle East Research Journal of Pharmaceutical Sciences, doi:10.36348/merjps.2023.v03i04.001
Aug 2023  
  Post
  Facebook
Share
  Source   PDF   All Studies   Meta AnalysisMeta
Quercetin for COVID-19
24th treatment shown to reduce risk in July 2021, now with p = 0.002 from 12 studies.
Lower risk for ICU, hospitalization, recovery, cases, and viral clearance.
No treatment is 100% effective. Protocols combine treatments.
5,100+ studies for 112 treatments. c19early.org
In Silico analysis molecular docking analysis predicting that quercetin, rutin, isorhamnetin, and tamarixetin bind well with SARS-CoV-2 Nsp13 helicase enzyme. The proposed mechanism of action is that these flavonoids can inhibit the ATPase and helicase activities of Nsp13, which normally unwinds RNA helices in an ATP-dependent manner during viral replication. This study provides evidence that quercetin and other flavonoids may have potential as SARS-CoV-2 antiviral agents by targeting and inhibiting the key Nsp13 helicase enzyme.
73 preclinical studies support the efficacy of quercetin for COVID-19:
45 In Silico studies1-45
22 In Vitro studies1,2,5,15,19,21,25,42,46-59
6 In Vivo animal studies5,19,52,55,60,61
In Silico studies predict inhibition of SARS-CoV-2, or minimization of side effects, with quercetin or metabolites via binding to the spikeA,3,9,10,22,24,25,30,38,39,41,42,62-64, MproB,3,7,9,11,13,15,17,18,20,23,24,30,34,36-38,42,43,45,63-65, RNA-dependent RNA polymeraseC,1,3,9,32,64, PLproD,3,37,45, ACE2E,22,23,28,37,41,63, TMPRSS2F,22, nucleocapsidG,3, helicaseH,3,29,34, endoribonucleaseI,39, NSP16/10J,6, cathepsin LK,26, Wnt-3L,22, FZDM,22, LRP6N,22, ezrinO,40, ADRPP,38, NRP1Q,41, EP300R,16, PTGS2S,23, HSP90AA1T,16,23, matrix metalloproteinase 9U,31, IL-6V,21,35, IL-10W,21, VEGFAX,35, and RELAY,35 proteins. In Vitro studies demonstrate inhibition of the MproB,15,46,51,59 protein, and inhibition of spike-ACE2 interactionZ,47. In Vitro studies demonstrate efficacy in Calu-3AA,50, A549AB,21, HEK293-ACE2+AC,58, Huh-7AD,25, Caco-2AE,49, Vero E6AF,19,42,49, mTECAG,52, and RAW264.7AH,52 cells. Animal studies demonstrate efficacy in K18-hACE2 miceAI,55, db/db miceAJ,52,61, BALB/c miceAK,60, and rats66. Quercetin reduced proinflammatory cytokines and protected lung and kidney tissue against LPS-induced damage in mice60, inhibits LPS-induced cytokine storm by modulating key inflammatory and antioxidant pathways in macrophages5, and inhibits SARS-CoV-2 ORF3a ion channel activity, which contributes to viral pathogenicity and cytotoxicity54.
Important missing comments or errors? Let us know.
a. The trimeric spike (S) protein is a glycoprotein that mediates viral entry by binding to the host ACE2 receptor, is critical for SARS-CoV-2's ability to infect host cells, and is a target of neutralizing antibodies. Inhibition of the spike protein prevents viral attachment, halting infection at the earliest stage.
b. The main protease or Mpro, also known as 3CLpro or nsp5, is a cysteine protease that cleaves viral polyproteins into functional units needed for replication. Inhibiting Mpro disrupts the SARS-CoV-2 lifecycle within the host cell, preventing the creation of new copies.
c. RNA-dependent RNA polymerase (RdRp), also called nsp12, is the core enzyme of the viral replicase-transcriptase complex that copies the positive-sense viral RNA genome into negative-sense templates for progeny RNA synthesis. Inhibiting RdRp blocks viral genome replication and transcription.
d. The papain-like protease (PLpro) has multiple functions including cleaving viral polyproteins and suppressing the host immune response by deubiquitination and deISGylation of host proteins. Inhibiting PLpro may block viral replication and help restore normal immune responses.
e. The angiotensin converting enzyme 2 (ACE2) protein is a host cell transmembrane protein that serves as the cellular receptor for the SARS-CoV-2 spike protein. ACE2 is expressed on many cell types, including epithelial cells in the lungs, and allows the virus to enter and infect host cells. Inhibition may affect ACE2's physiological function in blood pressure control.
f. Transmembrane protease serine 2 (TMPRSS2) is a host cell protease that primes the spike protein, facilitating cellular entry. TMPRSS2 activity helps enable cleavage of the spike protein required for membrane fusion and virus entry. Inhibition may especially protect respiratory epithelial cells, buy may have physiological effects.
g. The nucleocapsid (N) protein binds and encapsulates the viral genome by coating the viral RNA. N enables formation and release of infectious virions and plays additional roles in viral replication and pathogenesis. N is also an immunodominant antigen used in diagnostic assays.
h. The helicase, or nsp13, protein unwinds the double-stranded viral RNA, a crucial step in replication and transcription. Inhibition may prevent viral genome replication and the creation of new virus components.
i. The endoribonuclease, also known as NendoU or nsp15, cleaves specific sequences in viral RNA which may help the virus evade detection by the host immune system. Inhibition may hinder the virus's ability to mask itself from the immune system, facilitating a stronger immune response.
j. The NSP16/10 complex consists of non-structural proteins 16 and 10, forming a 2'-O-methyltransferase that modifies the viral RNA cap structure. This modification helps the virus evade host immune detection by mimicking host mRNA, making NSP16/10 a promising antiviral target.
k. Cathepsin L is a host lysosomal cysteine protease that can prime the spike protein through an alternative pathway when TMPRSS2 is unavailable. Dual targeting of cathepsin L and TMPRSS2 may maximize disruption of alternative pathways for virus entry.
l. Wingless-related integration site (Wnt) ligand 3 is a host signaling molecule that activates the Wnt signaling pathway, which is important in development, cell growth, and tissue repair. Some studies suggest that SARS-CoV-2 infection may interfere with the Wnt signaling pathway, and that Wnt3a is involved in SARS-CoV-2 entry.
m. The frizzled (FZD) receptor is a host transmembrane receptor that binds Wnt ligands, initiating the Wnt signaling cascade. FZD serves as a co-receptor, along with ACE2, in some proposed mechanisms of SARS-CoV-2 infection. The virus may take advantage of this pathway as an alternative entry route.
n. Low-density lipoprotein receptor-related protein 6 is a cell surface co-receptor essential for Wnt signaling. LRP6 acts in tandem with FZD for signal transduction and has been discussed as a potential co-receptor for SARS-CoV-2 entry.
o. The ezrin protein links the cell membrane to the cytoskeleton (the cell's internal support structure) and plays a role in cell shape, movement, adhesion, and signaling. Drugs that occupy the same spot on ezrin where the viral spike protein would bind may hindering viral attachment, and drug binding could further stabilize ezrin, strengthening its potential natural capacity to impede viral fusion and entry.
p. The Adipocyte Differentiation-Related Protein (ADRP, also known as Perilipin 2 or PLIN2) is a lipid droplet protein regulating the storage and breakdown of fats in cells. SARS-CoV-2 may hijack the lipid handling machinery of host cells and ADRP may play a role in this process. Disrupting ADRP's interaction with the virus may hinder the virus's ability to use lipids for replication and assembly.
q. Neuropilin-1 (NRP1) is a cell surface receptor with roles in blood vessel development, nerve cell guidance, and immune responses. NRP1 may function as a co-receptor for SARS-CoV-2, facilitating viral entry into cells. Blocking NRP1 may disrupt an alternative route of viral entry.
r. EP300 (E1A Binding Protein P300) is a transcriptional coactivator involved in several cellular processes, including growth, differentiation, and apoptosis, through its acetyltransferase activity that modifies histones and non-histone proteins. EP300 facilitates viral entry into cells and upregulates inflammatory cytokine production.
s. Prostaglandin G/H synthase 2 (PTGS2, also known as COX-2) is an enzyme crucial for the production of inflammatory molecules called prostaglandins. PTGS2 plays a role in the inflammatory response that can become severe in COVID-19 and inhibitors (like some NSAIDs) may have benefits in dampening harmful inflammation, but note that prostaglandins have diverse physiological functions.
t. Heat Shock Protein 90 Alpha Family Class A Member 1 (HSP90AA1) is a chaperone protein that helps other proteins fold correctly and maintains their stability. HSP90AA1 plays roles in cell signaling, survival, and immune responses. HSP90AA1 may interact with numerous viral proteins, but note that it has diverse physiological functions.
u. Matrix metalloproteinase 9 (MMP9), also called gelatinase B, is a zinc-dependent enzyme that breaks down collagen and other components of the extracellular matrix. MMP9 levels increase in severe COVID-19. Overactive MMP9 can damage lung tissue and worsen inflammation. Inhibition of MMP9 may prevent excessive tissue damage and help regulate the inflammatory response.
v. The interleukin-6 (IL-6) pro-inflammatory cytokine (signaling molecule) has a complex role in the immune response and may trigger and perpetuate inflammation. Elevated IL-6 levels are associated with severe COVID-19 cases and cytokine storm. Anti-IL-6 therapies may be beneficial in reducing excessive inflammation in severe COVID-19 cases.
w. The interleukin-10 (IL-10) anti-inflammatory cytokine helps regulate and dampen immune responses, preventing excessive inflammation. IL-10 levels can also be elevated in severe COVID-19. IL-10 could either help control harmful inflammation or potentially contribute to immune suppression.
x. Vascular Endothelial Growth Factor A (VEGFA) promotes the growth of new blood vessels (angiogenesis) and has roles in inflammation and immune responses. VEGFA may contribute to blood vessel leakiness and excessive inflammation associated with severe COVID-19.
y. RELA is a transcription factor subunit of NF-kB and is a key regulator of inflammation, driving pro-inflammatory gene expression. SARS-CoV-2 may hijack and modulate NF-kB pathways.
z. The interaction between the SARS-CoV-2 spike protein and the human ACE2 receptor is a primary method of viral entry, inhibiting this interaction can prevent the virus from attaching to and entering host cells, halting infection at an early stage.
aa. Calu-3 is a human lung adenocarcinoma cell line with moderate ACE2 and TMPRSS2 expression and SARS-CoV-2 susceptibility. It provides a model of the human respiratory epithelium, but many not be ideal for modeling early stages of infection due to the moderate expression levels of ACE2 and TMPRSS2.
ab. A549 is a human lung carcinoma cell line with low ACE2 expression and SARS-CoV-2 susceptibility. Viral entry/replication can be studied but the cells may not replicate all aspects of lung infection.
ac. HEK293-ACE2+ is a human embryonic kidney cell line engineered for high ACE2 expression and SARS-CoV-2 susceptibility.
ad. Huh-7 cells were derived from a liver tumor (hepatoma).
ae. Caco-2 cells come from a colorectal adenocarcinoma (cancer). They are valued for their ability to form a polarized cell layer with properties similar to the intestinal lining.
af. Vero E6 is an African green monkey kidney cell line with low/no ACE2 expression and high SARS-CoV-2 susceptibility. The cell line is easy to maintain and supports robust viral replication, however the monkey origin may not accurately represent human responses.
ag. mTEC is a mouse tubular epithelial cell line.
ah. RAW264.7 is a mouse macrophage cell line.
ai. A mouse model expressing the human ACE2 receptor under the control of the K18 promoter.
aj. A mouse model of obesity and severe insulin resistance leading to type 2 diabetes due to a mutation in the leptin receptor gene that impairs satiety signaling.
ak. A mouse model commonly used in infectious disease and cancer research due to higher immune response and susceptibility to infection.
Singh et al., 25 Aug 2023, peer-reviewed, 6 authors. Contact: office@kspublisher.com.
In Silico studies are an important part of preclinical research, however results may be very different in vivo.
This PaperQuercetinAll
Flavonoids as Potent Inhibitor of SARS-CoV-2 Nsp13 Helicase: Grid Based Docking Approach
Shikha Singh, Jitender K Malik, G Pavan Kumar, Sunil Kumar, Gyan Singh, Vinay Siroliya
Middle East Research Journal of Pharmaceutical Sciences, doi:10.36348/merjps.2023.v03i04.001
The corona virus (COVID-19) is an enveloped RNA virus with diverse origins in both people and wildlife. It has been determined that six separate species are the cause of human disease. Viral infections have a significant impact on human disease, and one of the most recent worldwide epidemics is the emergence of the new corona. The SS-RNA virus from the enveloped corona virus family is what caused the potentially lethal SARS (Severe Acute Respiratory Syndrome) virus. In many countries throughout the world, sickness is spreading quickly. As of March 26, 2020, there has been 462,684 confirmed cases and 20,834 fatalities documented abroad. COVID-19 was deemed a pandemic by the World Health Organisation (WHO) on March 11, 2020. Numerous drug studies are now underway, and some of the results are positive. The only way to combat the virus, however, is through preventative measures as there is no vaccination. The goal of the current study was to use a molecular docking approach to evaluate flavonoids's potential against SAR-CoV-2 infection. Elucidation of the proposed mechanism of action of natural flavonoid (Quercetin, Isorhametin, Rutin and Tamaraxiten) against SAR-CoV-2 infection.
References
Cousins, ChemDraw Ultra 9.0. CambridgeSoft, 100 CambridgePark Drive
Jain, Mujwar, Repurposing metocurine as main protease inhibitor to develop novel antiviral therapy for COVID-19, Structural chemistry
Kaur, Mujwar, Adlakha, In-silico analysis of riboswitch of Nocardia farcinica for design of its inhibitors and pharmacophores, International Journal of Computational Biology and Drug Design
Liskova, Samec, Koklesova, Samuel, Zhai et al., Flavonoids against the SARS-CoV-2 induced inflammatory storm, Biomedicine & Pharmacotherapy
Malik, Soni, Sharma, Sarankar, Hydroxychloroquine as potent inhibitor of COVID-19 main protease: Grid based docking approach, Eurasian Journal of Medicine and Oncology
Mishra, Pandey, Sharma, Malik, Mongre et al., Identifying the natural polyphenol catechin as a multi-targeted agent against SARS-CoV-2 for the plausible therapy of COVID-19: an integrated computational approach, Briefings in Bioinformatics
Mujwar, Computational repurposing of tamibarotene against triple mutant variant of SARS-CoV-2, Computers in Biology and Medicine
Mujwar, Deshmukh, Harwansh, Gupta, Gour, Drug repurposing approach for developing novel therapy against mupirocin-resistant Staphylococcus aureus, ASSAY and Drug Development Technologies
Russo, Moccia, Spagnuolo, Tedesco, Russo, Roles of flavonoids against coronavirus infection, Chemico-biological interactions
Sarvesh, Himesh, Jitender, Sanjay, Vimal, Corona: A review on current clinical sympathetic, J Appl Med Sci
Soni, Corona: Impact of Non-Living Virus to Living World, Saudi J Med Pharm Sci
Soni, Gautam, Sharma, Malik, Rifampicin as potent inhibitor of COVID-19 main protease: In-silico docking approach, Saudi Journal of Medical and Pharmaceutical Sciences
Soni, In-silico analysis to access the antibacterial effect of Rutin on E.coli: molecular docking approach, UJP
Soni, Malik, Sarankar, Soni, Rutin as a potent inhibitor of dihydrofolate reductase: A computational design and docking, EAS J. Pharm. Pharmacol
Soni, Mishra, Mishra, Mishra, Silibin as Potent Inhibitor of COVID-19 Main Protease: In-Silico Docking Approach, Journal of Molecular Pharmaceuticals and Regulatory Affairs
Soni, Sharma, Malik, Swarna Bhasma: A Hypothetical Approach to Fight against Corona Virus, South Asian Res J Pharm Sci
Soni, Sharma, Malik, Synergistic prophylaxis on COVID-19 by nature golden heart (Piper betle) & Swarna Bhasma, Asian Journal of Research in Dermatological Science
Vivek-Ananth, Krishnaswamy, Samal, Potential phytochemical inhibitors of SARS-CoV-2 helicase Nsp13: A molecular docking and dynamic simulation study, Molecular diversity
{ 'indexed': {'date-parts': [[2023, 9, 26]], 'date-time': '2023-09-26T05:45:58Z', 'timestamp': 1695707158052}, 'reference-count': 0, 'publisher': 'SASPR Edu International Pvt. Ltd', 'issue': '04', 'content-domain': {'domain': [], 'crossmark-restriction': False}, 'published-print': {'date-parts': [[2023, 8, 25]]}, 'abstract': '<jats:p>The corona virus (COVID-19) is an enveloped RNA virus with diverse origins in both ' 'people and wildlife. It has been determined that six separate species are the cause of human ' 'disease. Viral infections have a significant impact on human disease, and one of the most ' 'recent worldwide epidemics is the emergence of the new corona. The SS-RNA virus from the ' 'enveloped corona virus family is what caused the potentially lethal SARS (Severe Acute ' 'Respiratory Syndrome) virus. In many countries throughout the world, sickness is spreading ' 'quickly. As of March 26, 2020, there has been 462,684 confirmed cases and 20,834 fatalities ' 'documented abroad. COVID-19 was deemed a pandemic by the World Health Organisation (WHO) on ' 'March 11, 2020. Numerous drug studies are now underway, and some of the results are positive. ' 'The only way to combat the virus, however, is through preventative measures as there is no ' 'vaccination. The goal of the current study was to use a molecular docking approach to ' "evaluate flavonoids's potential against SAR-CoV-2 infection. Elucidation of the proposed " 'mechanism of action of natural flavonoid (Quercetin, Isorhametin, Rutin and Tamaraxiten) ' 'against SAR-CoV-2 infection.</jats:p>', 'DOI': '10.36348/merjps.2023.v03i04.001', 'type': 'journal-article', 'created': {'date-parts': [[2023, 9, 25]], 'date-time': '2023-09-25T05:09:02Z', 'timestamp': 1695618542000}, 'page': '51-65', 'source': 'Crossref', 'is-referenced-by-count': 0, 'title': 'Flavonoids as Potent Inhibitor of SARS-CoV-2 Nsp13 Helicase: Grid Based Docking Approach', 'prefix': '10.36348', 'volume': '3', 'author': [ {'given': 'Shikha', 'family': 'Singh', 'sequence': 'first', 'affiliation': []}, {'given': 'Jitender K', 'family': 'Malik', 'sequence': 'additional', 'affiliation': []}, {'given': 'G. Pavan', 'family': 'Kumar', 'sequence': 'additional', 'affiliation': []}, {'given': 'Sunil', 'family': 'Kumar', 'sequence': 'additional', 'affiliation': []}, {'given': 'Gyan', 'family': 'Singh', 'sequence': 'additional', 'affiliation': []}, {'given': 'Vinay', 'family': 'Siroliya', 'sequence': 'additional', 'affiliation': []}], 'member': '22072', 'published-online': {'date-parts': [[2023, 8, 25]]}, 'container-title': 'Middle East Research Journal of Pharmaceutical Sciences', 'original-title': [], 'deposited': { 'date-parts': [[2023, 9, 25]], 'date-time': '2023-09-25T05:09:03Z', 'timestamp': 1695618543000}, 'score': 1, 'resource': {'primary': {'URL': 'https://www.kspublisher.com/media/articles/MERJPS_34_51-65.pdf'}}, 'subtitle': [], 'short-title': [], 'issued': {'date-parts': [[2023, 8, 25]]}, 'references-count': 0, 'journal-issue': { 'issue': '04', 'published-online': {'date-parts': [[2023, 8, 26]]}, 'published-print': {'date-parts': [[2023, 8, 26]]}}, 'URL': 'http://dx.doi.org/10.36348/merjps.2023.v03i04.001', 'relation': {}, 'ISSN': ['2789-7702', '2958-213X'], 'container-title-short': 'Middle East Res J Pharm Sci', 'published': {'date-parts': [[2023, 8, 25]]}}
Loading..
Please send us corrections, updates, or comments. c19early involves the extraction of 100,000+ datapoints from thousands of papers. Community updates help ensure high accuracy. Treatments and other interventions are complementary. All practical, effective, and safe means should be used based on risk/benefit analysis. No treatment or intervention is 100% available and effective for all current and future variants. We do not provide medical advice. Before taking any medication, consult a qualified physician who can provide personalized advice and details of risks and benefits based on your medical history and situation. FLCCC and WCH provide treatment protocols.
  or use drag and drop   
Submit    
Post
Share
@CovidAnalysis
FAQ
Public domain CC0 1.0