TLR10 overexpression modulates immune response in A549 lung epithelial cells challenged with SARS-CoV-2 S and N proteins

DOI record: { "DOI": "10.3389/fimmu.2024.1490478", "ISSN": [ "1664-3224" ], "URL": "http://dx.doi.org/10.3389/fimmu.2024.1490478", "abstract": "<jats:p>Toll-like receptors (TLRs) play an important role in the recognition of viral particles and activation of the innate immune system, but their role in SARS-CoV-2 infection is still poorly characterized. In the present study, we investigated the role of Toll-like receptor 10 (TLR10) in modulating the immune response during SARS-CoV-2 infection. The results showed that overexpression of <jats:italic>TLR10</jats:italic> in A549 lung epithelial cells, immunostimulated with SARS-CoV-2 proteins S and N mainly downregulated proinflammatory cytokines and interferons and affected gene expression in the cocultured THP-1 monocytes. Our results suggest that TLR10 could mediate the extent of SARS-CoV-2 infection by downregulating the release of inflammatory cytokines and chemokines such as <jats:italic>CXCL10</jats:italic>, <jats:italic>IL6</jats:italic>, <jats:italic>IL8</jats:italic>, and <jats:italic>IFNβ</jats:italic>. Modulation of <jats:italic>TLR10</jats:italic> expression could have implications for the treatment of patients with severe COVID-19, in whom excessive inflammation leading to the development of acute respiratory distress syndrome (ARDS) is a key feature. However, further research is needed to fully understand the impact of modulating <jats:italic>TLR10</jats:italic> expression on the antiviral response and the overall balance of the immune response during SARS-CoV-2 infection.</jats:p>", "alternative-id": [ "10.3389/fimmu.2024.1490478" ], "author": [ { "affiliation": [], "family": "Knez", "given": "Špela", "sequence": "first" }, { "affiliation": [], "family": "Narat", "given": "Mojca", "sequence": "additional" }, { "affiliation": [], "family": "Ogorevc", "given": "Jernej", "sequence": "additional" } ], "container-title": "Frontiers in Immunology", "container-title-short": "Front. Immunol.", "content-domain": { "crossmark-restriction": true, "domain": [ "frontiersin.org" ] }, "created": { "date-parts": [ [ 2025, 1, 20 ] ], "date-time": "2025-01-20T05:24:27Z", "timestamp": 1737350667000 }, "deposited": { "date-parts": [ [ 2025, 1, 20 ] ], "date-time": "2025-01-20T05:24:29Z", "timestamp": 1737350669000 }, "indexed": { "date-parts": [ [ 2025, 1, 21 ] ], "date-time": "2025-01-21T05:03:09Z", "timestamp": 1737435789399, "version": "3.33.0" }, "is-referenced-by-count": 0, "issued": { "date-parts": [ [ 2025, 1, 20 ] ] }, "license": [ { "URL": "https://creativecommons.org/licenses/by/4.0/", "content-version": "vor", "delay-in-days": 0, "start": { "date-parts": [ [ 2025, 1, 20 ] ], "date-time": "2025-01-20T00:00:00Z", "timestamp": 1737331200000 } } ], "link": [ { "URL": "https://www.frontiersin.org/articles/10.3389/fimmu.2024.1490478/full", "content-type": "unspecified", "content-version": "vor", "intended-application": "similarity-checking" } ], "member": "1965", 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