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Individual ingredients of NP-101 (Thymoquinone formula) inhibit SARS-CoV-2 pseudovirus infection

Maen et al., Frontiers in Pharmacology, doi:10.3389/fphar.2024.1291212
Feb 2024  
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In Vitro study showing that components of thymoquinone formulation NP-101, including thymoquinone, oleic acid, linoleic acid, and palmitic acid, showed dose-dependent inhibition of SARS-CoV-2 variants in a pseudovirus model. Combinations of TQ and fatty acids also inhibited variants, with palmitic acid showing increased potency. A large randomized trial is planned to validate the potential of TQF for COVID-19 treatment. The results suggest thymoquinone and fatty acids in NP-101 could provide therapeutic benefits, likely by blocking viral entry via the ACE2 receptor.
Maen et al., 6 Feb 2024, peer-reviewed, 15 authors. Contact:,
In Vitro studies are an important part of preclinical research, however results may be very different in vivo.
This PaperNigella SativaAll
Individual ingredients of NP-101 (Thymoquinone formula) inhibit SARS-CoV-2 pseudovirus infection
Abdelrahim Maen, Betul Gok Yavuz, Yehia I Mohamed, Abdullah Esmail, Jianming Lu, Amr Mohamed, Asfar S Azmi, Mohamed Kaseb, Osama Kasseb, Dan Li, Michelle Gocio, Mehmet Kocak, Abdelhafez Selim, Qing Ma, Ahmed O Kaseb
Frontiers in Pharmacology, doi:10.3389/fphar.2024.1291212
Thymoquinone TQ, an active ingredient of Nigella Sativa, has been shown to inhibit COVID-19 symptoms in clinical trials. Thymoquinone Formulation (TQF or NP-101) is developed as a novel enteric-coated medication derivative from Nigella Sativa. TQF consists of TQ with a favorable concentration and fatty acids, including palmitic, oleic, and linoleic acids. In this study, we aimed to investigate the roles of individual ingredients of TQF on infection of SARS-CoV-2 variants in-vitro, by utilizing Murine Leukemia Virus (MLV) based pseudovirus particles. We demonstrated that NP-101, TQ, and other individual ingredients, including oleic, linoleic, and palmitic acids inhibited SARS-CoV-2 infection in the MLV-based pseudovirus model. A large, randomized phase 2 study of NP-101 is planned in outpatient COVID-19 patients.
Author contributions AbM: Conceptualization, Data curation, Formal Analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing-original draft, Writing-review and editing. BG: Conceptualization, Investigation, Software, Writing-original draft, Writing-review and editing. YM: Conceptualization, Data Conflict of interest Author JL was employed by Codex BioSolutions Inc. Authors MoK, OK, and MG were employed by Novatek Pharmaceuticals, Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. Publisher's note All claims expressed in this article are solely those of the authors and do not necessarily represent those of their Supplementary material The Supplementary Material for this article can be found online at: full#supplementary-material
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