Analgesics
Antiandrogens
Azvudine
Bromhexine
Budesonide
Colchicine
Conv. Plasma
Curcumin
Famotidine
Favipiravir
Fluvoxamine
Hydroxychlor..
Ivermectin
Lifestyle
Melatonin
Metformin
Minerals
Molnupiravir
Monoclonals
Naso/orophar..
Nigella Sativa
Nitazoxanide
Paxlovid
Quercetin
Remdesivir
Thermotherapy
Vitamins
More

Other
Feedback
Home
Top
Abstract
All andrographolide studies
Meta analysis
 
Feedback
Home
next
study
previous
study
c19early.org COVID-19 treatment researchAndrographolideAndrographol.. (more..)
Melatonin Meta
Metformin Meta
Azvudine Meta
Bromhexine Meta Molnupiravir Meta
Budesonide Meta
Colchicine Meta
Conv. Plasma Meta Nigella Sativa Meta
Curcumin Meta Nitazoxanide Meta
Famotidine Meta Paxlovid Meta
Favipiravir Meta Quercetin Meta
Fluvoxamine Meta Remdesivir Meta
Hydroxychlor.. Meta Thermotherapy Meta
Ivermectin Meta

All Studies   Meta Analysis    Recent:   

The role of andrographolide and its derivative in COVID-19 associated proteins and immune system

Dey et al., Research Square, doi:10.21203/rs.3.rs-35800/v1
Jun 2020  
  Post
  Facebook
Share
  Source   PDF   All Studies   Meta AnalysisMeta
In Silico study showing that andrographolide and its derivative 14-deoxy-11,12-didehydroandrographolide from Andrographis paniculata may be beneficial for COVID-19 by binding to key SARS-CoV-2 proteins and modulating immune pathways. Authors found that both compounds had high binding affinity to the SARS-CoV-2 papain-like protease (PLpro), main protease (3CLpro), and spike protein. Network pharmacology analysis revealed that the compounds modulated multiple immune-related pathways, including the chemokine signaling pathway, Rap1 signaling pathway, and cytokine-cytokine receptor interactions.
11 preclinical studies support the efficacy of andrographolide for COVID-19:
Dey et al., 18 Jun 2020, preprint, 7 authors. Contact: manish.nriashrd@gmail.com.
In Silico studies are an important part of preclinical research, however results may be very different in vivo.
This PaperAndrographol..All
The role of andrographolide and its derivative in COVID-19 associated proteins and immune system
Yadu Nandan Dey, Pukar Khanal, B M Patil, Manish M Wanjari, Bhavana Srivast, Shailendra S Gurav, Sudesh N Gaidhani
doi:10.21203/rs.3.rs-35800/v1
Aim: In view of the strong immunomodulatory and antiviral activity of andrographolide and its derivative, the present study aimed to investigate the binding a nities of andrographolide and its derivative 14deoxy-11,12-didehydroandrographolide with 3 major targets of COVID-19 i.e. 3CLpro, PLpro and spike protein followed by their gene-set enrichment analysis with special reference to immune modulation. Materials and methods: SMILES of the compounds were retrieved from DigepPred database and the proteins identi ed were queried in STRING to evaluate the protein-protein interaction and modulated pathways were identi ed concerning the KEGG database. Drug-likeness and ADMET pro les were evaluated using MolSoft and admet SAR 2.0, respectively. Molecular docking was carried using autodock 4.0. Results: Andrographolide and 14-Deoxy-11,12-didehydroandrographolide were predicted to have a high binding a nity with papain-like protease i.e. -6.7 kcal/mol and -6.5 kcal/mol, respectively while they interact with equal binding energies with 3clpro (-6.8 kcal/mol) and spike protein (-6.9 kcal/mol). Network pharmacology analysis revealed that both compounds modulated the immune system through the regulation of chemokine signaling pathway, Rap1 signaling pathway, Cytokine-cytokine receptor interaction, MAPK signaling pathway, NF-kappa B signaling pathway, Rassignaling pathway, p53 signaling pathway, HIF-1 signaling pathway, and Natural killer cell-mediated cytotoxicity. Although the 14deoxy-11,12-didehydroandrographolide scored higher drug-likeness character, it showed less potency to interaction with targeted proteins of COVID-19. Conclusion: The study suggests the strong interaction of the andrographolide and its derivative 14-deoxy-11,12-didehydroandrographolide against target proteins associated with COVID-19. Further, network pharmacology analysis elucidated the different pathways of immunomodulation. However, clinical research should be conducted to con rm the current ndings.
References
Bahmani, Saki, Shahsavari, Ra Eian-Kopaei, Sepahvand et al., Identi cation of medicinal plants effective in infectious diseases in Urmia, northwest of Iran, Asian Pac J Trop Biomed
Chen, Michaelis, Hsu, Tsai, Yang et al., ToonasinensisRoem tender leaf extract inhibits SARS coronavirus replication, J Ethnopharmacol
Chen, Xue, Ye, Fang, Liu et al., Activity of andrographolide and its derivatives against in uenza virus in vivo and in vitro, Biol Pharm Bull
Chi, Flavell, Studies on MAP Kinase signaling in the immune system, Methods Mol Biol
Chikhale, Sinha, Patil, Prasad, Shrivastava et al., SARS-CoV-2 host entry and replication inhibitors from Indian ginseng: in silico approach, J Biomole Stru Dyna, doi:10.1080/07391102.2020.1778539
Churiyah, Ob, Rofaani, Tarwadi, Antiviral and immunostimulant activities of Andrographis paniculata, HAYATI J Biosci
Edwin, Vasantha-Srinivasan, Senthil-Nathan, Thanigaivel, Ponsankar et al., Anti-dengue e cacy of bioactive andrographolide from Andrographis paniculata (Lamiales: Acanthaceae) against the primary dengue vector Aedes aegypti (Diptera: Culicidae), Acta Trop
Gupta, Mishra, Ganju, Broad-spectrum antiviral properties of andrographolide, Arch Virol
Huang, Wang, Li, Ren, Zhao et al., Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China, Lancet
Kortlever, Sodir, Wilson, Burkhart, Pellegrinet et al., Myc Cooperates with Ras by Programming In ammation and Immune Suppression, Cell
Lagunin, Ivanov, Rudik, Filimonov, Poroikov, DIGEP-Pred: web service for in silico prediction of drug-induced gene expression pro les based on structural formula, Bioinformatics
Lee, Tseng, Young, Sun, Wang et al., Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells, Br J Pharmacol
Lin, Chen, Duh, Chang, Liu, Inhibition of the Epstein-barr virus lytic cycle by andrographolide, Biol Pharm Bull
Lin, Tsai, Tsai, Lai, Wan et al., Anti-SARS coronavirus 3C-like protease effects of Isatisindigotica root and plant-derived phenolic compounds, Antiviral Research
Magrone, Magrone, Jirillo, Focus on receptors for coronaviruses with special reference to angiotensin-converting enzyme 2 as a potential prug target -A perspective, Endocr Metab Immune Disord Drug Targets
Mahady, Medicinal plants for the prevention and treatment of bacterial infections, Curr Pharm Des
Mishra, Dash, Dey, Andrographolide: A novel antimalarial diterpene lactone compound from Andrographis paniculata and its interaction with curcumin and artesunate, J Trop Med
Mopuri, Ganjayi, Banavathy, Parim, Meriga, Evaluation of anti-obesity activities of ethanolic extract of Terminalia paniculata bark on high fat diet-induced obese rats, BMC Complement Altern Med
Morris, Huey, Lindstrom, Sanner, Belew et al., AutoDock4 and AutoDockTools4: Automated docking with selective receptor exibility, J Comput Chem
Opitz, Van Laak, Eitel, Suttorp, Innate immune recognition in infectious and noninfectious diseases of the lung, Am J RespirCrit Care Med
Pongtuluran, Rofaani, Antiviral and immunostimulant activities of Andrographis paniculata, HAYATI J Biosci
Puri, Saxena, Saxena, Srivastava, Tandon, Immunostimulating agents from Andrographis paniculata, J Nat Prod
Ryu, Jeong, Kim, Kim, Park et al., Bi avonoids from Torreya nucifera displaying SARS-CoV 3CL(pro) inhibition, Bioorganic & Medicinal Chemistry
Schwede, Kopp, Guex, Peitsch, SWISS-MODEL: An automated protein homology-modeling server
Seniya, Shrivastava, Singh, Khan, Analyzing the interaction of a herbal compound Andrographolide from Andrographis paniculata as a folklore against swine u (H1N1), Asian Pac J Trop Dis
Sinha, Prasad, Gurav, Patil, …shakyaa, Identi cation of Bioactive Compounds from Glycyrrhiza glabra as Possible Inhibitor of SARS-CoV-2 Spike Glycoprotein and Non-structural Protein-15: A Pharmacoinformatics Study, J Biomole Stru Dyna, doi:10.1080/07391102.2020.1779132
Sinha, Shakya, Prasad, Singh, Gurav et al., An in-silico evaluation of different Saikosaponins for their potency against SARS-CoV-2 using NSP15 and fusion spike glycoprotein as targets, J Biomole Stru Dyn
Sofowora, Ogunbodede, Onayade, The role and place of medicinal plants in the strategies for disease prevention, Afr J Tradit Complement Altern Med
Sokol, Luster, The chemokine system in innate immunity, Cold Spring HarbPerspect Biol, doi:10.1101/cshperspect.a016303
Szklarczyk, Morris, Cook, The STRING database in 2017: quality-controlled proteinprotein association networks, made broadly accessible, Nucleic Acids Res
Wan, Yi, Fan, Characteristics of lymphocyte subsets and cytokines in peripheral blood of 123 hospitalized patients with 2019 novel coronavirus pneumonia (NCP), medRxiv, doi:10.1101/2020.02.10.20021832
Wang, Hu, Hu, Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China, JAMA
Wang, Wang, Dong, Immunomodulatory activity of andrographolide on macrophage activation and speci c antibody response, ActaPharmacol Sin, doi:10.1038/aps.2009.205
Wen, Shyur, Jan, Liang, Kuo et al., Traditional Chinese medicine herbal extracts of Cibotiumbarometz, Gentianascabra, Dioscoreabatatas, Cassia tora, and Taxilluschinensis inhibit SARS CoV replication, J Trad Complement Med
Wiart, Kumar, Yusof, Hamimah, Fauzi et al., Antiviral properties of ent-labdene diterpenes of Andrographis paniculata Nees, inhibitors of herpes simplex virus type I, Phytother Res
Wintachai, Kaur, Lee, Ramphan, Kuadkitkan et al., Activity of andrographolide against chikungunya virus infection, Sci Rep
Xu, Shi, Wang, Pathological ndings of COVID-19 associated with acute respiratory distress syndrome, Lancet Respir Med
Yang, Lou, Sun, Admet SAR 2.0: web-service for prediction and optimization of chemical ADMET properties, Bioinformatics
Zhang, Tan, Anti-diabetic property of ethanolic extract of Andrographis paniculata in streptozotocin-diabetic rats, Acta Pharmacol Sin
Zhu, Zhang, Li, Yang, Song et al., A novel coronavirus from patients with pneumonia in China, 2019, N Engl J Med
{ 'DOI': '10.21203/rs.3.rs-35800/v1', 'URL': 'http://dx.doi.org/10.21203/rs.3.rs-35800/v1', 'abstract': '<jats:title>Abstract</jats:title>\n' ' <jats:p><jats:bold>Aim: </jats:bold>In view of the strong immunomodulatory and ' 'antiviral activity of andrographolide and its derivative, the present study aimed to ' 'investigate the binding affinities of andrographolide and its derivative ' '14-deoxy-11,12-didehydroandrographolide with 3 major targets of COVID-19 i.e. 3CLpro, PLpro ' 'and spike protein followed by their gene-set enrichment analysis with special reference to ' 'immune modulation.<jats:bold>Materials and methods:</jats:bold> SMILES of the compounds were ' 'retrieved from DigepPred database and the proteins identified were queried in STRING to ' 'evaluate the protein-protein interaction and modulated pathways were identified concerning ' 'the KEGG database. Drug-likeness and ADMET profiles were evaluated using MolSoft and admet ' 'SAR 2.0, respectively. Molecular docking was carried using autodock 4.0.<jats:bold>Results: ' '</jats:bold>Andrographolide and 14-Deoxy-11,12-didehydroandrographolide were predicted to ' 'have a high binding affinity with papain-like protease i.e. -6.7 kcal/mol and -6.5 kcal/mol, ' 'respectively while they interact with equal binding energies with 3clpro (-6.8 kcal/mol) and ' 'spike protein (-6.9 kcal/mol). Network pharmacology analysis revealed that both compounds ' 'modulated the immune system through the regulation of chemokine signaling pathway, Rap1 ' 'signaling pathway, Cytokine-cytokine receptor interaction, MAPK signaling pathway, NF-kappa B ' 'signaling pathway, Rassignaling pathway, p53 signaling pathway, HIF-1 signaling pathway, and ' 'Natural killer cell-mediated cytotoxicity. Although the ' '14-deoxy-11,12-didehydroandrographolide scored higher drug-likeness character, it showed less ' 'potency to interaction with targeted proteins of COVID-19.<jats:bold>Conclusion: ' '</jats:bold>The study suggests the strong interaction of the andrographolide and its ' 'derivative 14-deoxy-11,12-didehydroandrographolide against target proteins associated with ' 'COVID-19. Further, network pharmacology analysis elucidated the different pathways of ' 'immunomodulation. However, clinical research should be conducted to confirm the current ' 'findings.</jats:p>', 'accepted': {'date-parts': [[2020, 6, 15]]}, 'author': [ { 'affiliation': [ { 'name': 'School of Pharmaceutical Technology, Adamas University, ' 'Kolkata-700126, West Bengal, India'}], 'family': 'Dey', 'given': 'Yadu Nandan', 'sequence': 'first'}, { 'affiliation': [ { 'name': 'Department of Pharmacology and Toxicology, KLE College of ' 'Pharmacy, Belagavi, KLE Academy of Higher Education and Research ' '(KAHER), Belagavi-590010, Karnataka, India 3Regional Ayurveda ' 'Research Institute for Drug Development, Gwalior-474009, Madhya ' 'Pradesh, India'}], 'family': 'Khanal', 'given': 'Pukar', 'sequence': 'additional'}, { 'affiliation': [ { 'name': 'Department of Pharmacology and Toxicology, KLE College of ' 'Pharmacy, Belagavi, KLE Academy of Higher Education and Research ' '(KAHER), Belagavi-590010, Karnataka, India 3Regional Ayurveda ' 'Research Institute for Drug Development, Gwalior-474009, Madhya ' 'Pradesh, India'}], 'family': 'Patil', 'given': 'B. M.', 'sequence': 'additional'}, { 'ORCID': 'http://orcid.org/0000-0003-0691-2652', 'affiliation': [ { 'name': 'Regional Ayurveda Research Institute for Drug Development, ' 'Gwalior-474009, Madhya Pradesh, India'}], 'authenticated-orcid': False, 'family': 'Wanjari', 'given': 'Manish M.', 'sequence': 'additional'}, { 'affiliation': [ { 'name': 'Regional Ayurveda Research Institute for Drug Development, ' 'Gwalior-474009, Madhya Pradesh, India'}], 'family': 'Srivast', 'given': 'Bhavana', 'sequence': 'additional'}, { 'affiliation': [{'name': 'Goa College of Pharmacy, Panjim-403001, Goa, India'}], 'family': 'Gurav', 'given': 'Shailendra S.', 'sequence': 'additional'}, { 'affiliation': [ { 'name': 'Central Council for Research in Ayurvedic Sciences, New ' 'Delhi-110058, India'}], 'family': 'Gaidhani', 'given': 'Sudesh N.', 'sequence': 'additional'}], 'container-title': [], 'content-domain': {'crossmark-restriction': False, 'domain': []}, 'created': {'date-parts': [[2020, 6, 18]], 'date-time': '2020-06-18T16:54:53Z', 'timestamp': 1592499293000}, 'deposited': { 'date-parts': [[2020, 6, 18]], 'date-time': '2020-06-18T16:54:54Z', 'timestamp': 1592499294000}, 'group-title': 'In Review', 'indexed': {'date-parts': [[2023, 11, 1]], 'date-time': '2023-11-01T07:57:31Z', 'timestamp': 1698825451643}, 'is-referenced-by-count': 1, 'issued': {'date-parts': [[2020, 6, 18]]}, 'license': [ { 'URL': 'https://creativecommons.org/licenses/by/4.0/', 'content-version': 'unspecified', 'delay-in-days': 0, 'start': { 'date-parts': [[2020, 6, 18]], 'date-time': '2020-06-18T00:00:00Z', 'timestamp': 1592438400000}}], 'link': [ { 'URL': 'https://www.researchsquare.com/article/rs-35800/v1', 'content-type': 'text/html', 'content-version': 'vor', 'intended-application': 'text-mining'}, { 'URL': 'https://www.researchsquare.com/article/rs-35800/v1.html', 'content-type': 'unspecified', 'content-version': 'vor', 'intended-application': 'similarity-checking'}], 'member': '8761', 'original-title': [], 'posted': {'date-parts': [[2020, 6, 18]]}, 'prefix': '10.21203', 'published': {'date-parts': [[2020, 6, 18]]}, 'publisher': 'Research Square Platform LLC', 'reference-count': 0, 'references-count': 0, 'relation': {}, 'resource': {'primary': {'URL': 'https://www.researchsquare.com/article/rs-35800/v1'}}, 'score': 1, 'short-title': [], 'source': 'Crossref', 'subtitle': [], 'subtype': 'preprint', 'title': 'The role of andrographolide and its derivative in COVID-19 associated proteins and immune system', 'type': 'posted-content'}
Loading..
Please send us corrections, updates, or comments. c19early involves the extraction of 100,000+ datapoints from thousands of papers. Community updates help ensure high accuracy. Treatments and other interventions are complementary. All practical, effective, and safe means should be used based on risk/benefit analysis. No treatment or intervention is 100% available and effective for all current and future variants. We do not provide medical advice. Before taking any medication, consult a qualified physician who can provide personalized advice and details of risks and benefits based on your medical history and situation. FLCCC and WCH provide treatment protocols.
  or use drag and drop   
Submit