Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
et al., Nutrients, doi:10.3390/nu13020328 (In Vitro)
, Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro, Nutrients, doi:10.3390/nu13020328 (In Vitro)
Caco-2 and Vero E6 In Vitro study showing lactoferrin enhances the antiviral immune response, partially inhibits SARS-CoV-2 infection in Caco-2 intestinal epithelial cells, and modulates cytokine production.7 In Vitro studies support the efficacy of lactoferrin [Andreu, Cutone, Mirabelli, Ostrov, Piacentini, Salaris, Yazawa].
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Cutone et al., Research Square, doi:10.21203/rs.3.rs-1605740/v1,
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Ostrov et al., Pathogens, doi:10.3390/pathogens10111514,
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Salaris et al., 23 Jan 2021, peer-reviewed, 9 authors.
Contact:
paola.brun.1@unipd.it (corresponding author), claudio.salaris@studenti.unipd.it, alice.bertolini@studenti.unipd.it, ignazio.castagliuolo@unipd.it, melania.scarpa@iov.veneto.it, marina.elli@aat-taa.eu, simone.guglielmetti@unimi.it, fabrizio.pregliasco@unimi.it, corrado.blandizzi@med.unipi.it.
In Vitro studies are an important part of preclinical research, however results may be very different in vivo.
Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
Nutrients, doi:10.3390/nu13020328
SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection.
Conflicts of Interest: The authors declare no conflict of interest.
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