Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro
et al., Nutrients, doi:10.3390/nu13020328 (In Vitro)
, Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro, Nutrients, doi:10.3390/nu13020328 (In Vitro)
Caco-2 and Vero E6 In Vitro study showing lactoferrin enhances the antiviral immune response, partially inhibits SARS-CoV-2 infection in Caco-2 intestinal epithelial cells, and modulates cytokine production.5 In Vitro studies support the efficacy of lactoferrin [Cutone, Mirabelli, Ostrov, Piacentini, Salaris].
1.
Cutone et al., Research Square, doi:10.21203/rs.3.rs-1605740/v1,
Lactoferrin binding to Sars-CoV-2 Spike glycoprotein protects host from infection, inflammation and iron dysregulation.,
https://www.researchsquare.com/article/rs-1605740/v1.
2.
Mirabelli et al., Proceedings of the National Academy of Sciences, doi:10.1073/pnas.2105815118,
Morphological cell profiling of SARS-CoV-2 infection identifies drug repurposing candidates for COVID-19,
https://www.pnas.org/content/118/36/e2105815118.
3.
Ostrov et al., Pathogens, doi:10.3390/pathogens10111514,
Highly Specific Sigma Receptor Ligands Exhibit Anti-Viral Properties in SARS-CoV-2 Infected Cells,
https://www.mdpi.com/2076-0817/10/11/1514/htm.
Salaris et al., 23 Jan 2021, peer-reviewed, 9 authors.
Contact:
paola.brun.1@unipd.it (corresponding author), claudio.salaris@studenti.unipd.it, alice.bertolini@studenti.unipd.it, ignazio.castagliuolo@unipd.it, melania.scarpa@iov.veneto.it, marina.elli@aat-taa.eu, simone.guglielmetti@unimi.it, fabrizio.pregliasco@unimi.it, corrado.blandizzi@med.unipi.it.
In Vitro studies are an important part of preclinical research, however results may be very different in vivo.
Abstract: nutrients
Article
Protective Effects of Lactoferrin against SARS-CoV-2 Infection
In Vitro
Claudio Salaris 1,† , Melania Scarpa 2,† , Marina Elli 3 , Alice Bertolini 1 , Simone Guglielmetti 4 ,
Fabrizio Pregliasco 5 , Corrado Blandizzi 6 , Paola Brun 1, * and Ignazio Castagliuolo 1
1
2
3
4
5
6
*
†
Citation: Salaris, C.; Scarpa, M.; Elli,
M.; Bertolini, A.; Guglielmetti, S.;
Pregliasco, F.; Blandizzi, C.; Brun, P.;
Castagliuolo, I. Protective Effects of
Lactoferrin against SARS-CoV-2
Infection In Vitro. Nutrients 2021, 13,
328. https://doi.org/10.3390/
Department of Molecular Medicine, University of Padua, 35121 Padua, Italy;
claudio.salaris@studenti.unipd.it (C.S.); alice.bertolini@studenti.unipd.it (A.B.);
ignazio.castagliuolo@unipd.it (I.C.)
Laboratory of Advanced Translational Research, Veneto Institute of Oncology IOV-IRCCS, 35128 Padua, Italy;
melania.scarpa@iov.veneto.it
AAT-Advanced Analytical Technologies S.r.l., Fiorenzuola d’Arda, 29122 Piacenza, Italy;
marina.elli@aat-taa.eu
Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan,
20122 Milan, Italy; simone.guglielmetti@unimi.it
IRCCS Istituto Ortopedico Galeazzi, University of Milan, 20136 Milan, Italy; fabrizio.pregliasco@unimi.it
Unit of Pharmacology and Pharmacovigilance, Department of Clinical and Experimental Medicine,
University of Pisa, 56126 Pisa, Italy; corrado.blandizzi@med.unipi.it
Correspondence: paola.brun.1@unipd.it
These authors contributed equally to this work.
Abstract: SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin
(LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention
of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by
qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for
SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined
by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine
production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1,
TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection
and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune
modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection.
nu13020328
Keywords: lactoferrin; SARS-CoV-2; antiviral immunity; COVID-19
Received: 16 December 2020
Accepted: 21 January 2021
Published: 23 January 2021
Loading..
Please send us corrections, updates, or comments. Vaccines and
treatments are complementary. All practical, effective, and safe means should
be used based on risk/benefit analysis. No treatment, vaccine, or intervention
is 100% available and effective for all current and future variants. We do not
provide medical advice. Before taking any medication, consult a qualified
physician who can provide personalized advice and details of risks and
benefits based on your medical history and situation. FLCCC and WCH
provide treatment protocols.