Lactoferrin binding to Sars-CoV-2 Spike glycoprotein protects host from infection, inflammation and iron dysregulation.
Cutone et al.,
Lactoferrin binding to Sars-CoV-2 Spike glycoprotein protects host from infection, inflammation and iron..,
Research Square, doi:10.21203/rs.3.rs-1605740/v1 (Preprint) (In Vitro)
Vero E6, Caco-2, and THP-1
In Vitro study showing lactoferrin inhibited SARS-CoV-2 spike pseudovirus. Nutraceutically available bovine lactoferrin was more effective than human lactoferrin.
5 In Vitro studies support the efficacy of lactoferrin
[Cutone, Mirabelli, Ostrov, Piacentini, Salaris].
Cutone et al., 17 May 2022, Italy, preprint, 12 authors.
Contact:
musci@unimol.it.
In Vitro studies are an important part of preclinical research, however results may be very different in vivo.
Abstract: Lactoferrin binding to Sars-CoV-2 Spike glycoprotein
protects host from infection, in ammation and iron
dysregulation.
Antimo Cutone
University of Molise Department of Bioscience and Environment: Universita degli Studi del Molise
Dipartimento di Bioscienze e Territorio
Luigi Rosa
Sapienza University of Rome Department of Public Health and Infectious Diseases
Maria Carmela Bonaccorsi di Patti
Sapienza University of Rome Department of Biochemical Sciences
Federico Iacovelli
Univ of Rome Tor Vergata Department of Biologyersity
Maria Pia Conte
Sapienza University of Rome Department of Public Health and Infectious Diseases
Giusi Ianiro
University of Molise Department of Bioscience and Territory: Universita degli Studi del Molise
Dipartimento di Bioscienze e Territorio
Alice Romeo
University of Rome Tor Vergata Department of Biology
Elena Campione
University of Rome Tor Vergata Dermatology Unit
Luca Bianchi
University of Rome Tor Vergata Dermatology Unit
Piera Valenti
Sapienza University of Rome Department of Public Health and Infectious Diseases
Mattia Falconi
University of Rome Tor Vergata Department of Biology
Giovanni Musci ( musci@unimol.it )
University of Molise Department of Bioscience and Territory: Universita degli Studi del Molise
Dipartimento di Bioscienze e Territorio https://orcid.org/0000-0002-5196-709X
Research Article
Keywords: Lactoferrin, SARS-CoV-2, COVID-19, Iron homeostasis, In ammation
Posted Date: May 17th, 2022
DOI: https://doi.org/10.21203/rs.3.rs-1605740/v1
License: This work is licensed under a Creative Commons Attribution 4.0 International License.
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Lactoferrin binding to Sars-CoV-2 Spike glycoprotein protects host from infection,
inflammation and iron dysregulation.
Antimo Cutone1*, Luigi Rosa2*, Maria Carmela Bonaccorsi di Patti3, Federico Iacovelli4, Maria Pia
Conte2, Giusi Ianiro1, Alice Romeo4, Elena Campione5, Luca Bianchi5, Piera Valenti2, Mattia
Falconi4 and Giovanni Musci1#
1
Department of Biosciences and Territory, University of Molise, Pesche, Italy.
2
Department of Public Health and Infectious Diseases, Sapienza University of Rome, Italy.
3
Department of Biochemical Sciences, Sapienza University of Rome, Italy.
4
Department of Biology, University of Rome “Tor Vergata”, Rome, Italy.
5
Dermatology Unit, University of Rome “Tor Vergata”, Rome, Italy.
*
These authors equally contributed to the manuscript
#
Corresponding author: Giovanni Musci (musci@unimol.it).
Keywords: Lactoferrin, SARS-CoV-2, COVID-19, Iron homeostasis, Inflammation
1
Abstract
The anti-SARS-Cov-2 activity of the iron-binding protein Lactoferrrin has been investigated in
epithelial and macrophagic cell models using a Pseudovirus decorated with the SARS-CoV-2 Spike
glycoprotein. The human and, even more, the nutraceutically available bovine Lactoferrin inhibit
pseudoviral infection in all cellular models tested. The bovine protein efficiently counteracts the
deleterious effects of purified Spike on iron and inflammatory homeostasis, as shown by restored
levels of the main proteins of the iron-handling system and, in the case of macrophagic THP-1 cells,
of the proinflammatory cytokines IL-1β and IL-6. A direct interaction between Lactoferrin and Spike
is likely at the basis of the observed effects, as demonstrated by an in vitro pull-down assay. Finally,
in silico approaches have been applied to analyze the interactions of human and bovine Lactoferrins
with Transferrin Receptor 1, a potential..
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