Alkalinization
Analgesics..
Antiandrogens..
Bromhexine
Budesonide
Cannabidiol
Colchicine
Conv. Plasma
Curcumin
Ensovibep
Famotidine
Favipiravir
Fluvoxamine
Hydroxychlor..
Iota-carragee..
Ivermectin
Lactoferrin
Lifestyle..
Melatonin
Metformin
Molnupiravir
Monoclonals..
Nigella Sativa
Nitazoxanide
Nitric Oxide
Paxlovid
Peg.. Lambda
Povidone-Iod..
Quercetin
Remdesivir
Vitamins..
Zinc

Other
Feedback
Home
Home   COVID-19 treatment studies for Lactoferrin  COVID-19 treatment studies for Lactoferrin  C19 studies: Lactoferrin  Lactoferrin   Select treatmentSelect treatmentTreatmentsTreatments
Alkalinization Meta Lactoferrin Meta
Melatonin Meta
Bromhexine Meta Metformin Meta
Budesonide Meta Molnupiravir Meta
Cannabidiol Meta
Colchicine Meta Nigella Sativa Meta
Conv. Plasma Meta Nitazoxanide Meta
Curcumin Meta Nitric Oxide Meta
Ensovibep Meta Paxlovid Meta
Famotidine Meta Peg.. Lambda Meta
Favipiravir Meta Povidone-Iod.. Meta
Fluvoxamine Meta Quercetin Meta
Hydroxychlor.. Meta Remdesivir Meta
Iota-carragee.. Meta
Ivermectin Meta Zinc Meta

Other Treatments Global Adoption
All Studies   Meta Analysis   Recent:  

Evaluation of SARS-CoV-2 isolation in cell culture from nasal/nasopharyngeal swabs or saliva specimens of patients with COVID-19

Yazawa et al., Research Square, doi:10.21203/rs.3.rs-2676422/v1 (Preprint) (In Vitro)
Yazawa et al., Evaluation of SARS-CoV-2 isolation in cell culture from nasal/nasopharyngeal swabs or saliva specimens of.., Research Square, doi:10.21203/rs.3.rs-2676422/v1 (Preprint) (In Vitro)
Mar 2023   Source   PDF  
  Twitter
  Facebook
Share
  All Studies   Meta
Analysis of nasal/nasopharyngeal swabs and saliva specimens, showing significantly lower isolation efficiency of SARS-CoV-2 in saliva specimens. In Vitro study showing that lactoferrin and amylase, components of saliva, were found to inhibit SARS-CoV-2 pseudotyped virus infection.
7 In Vitro studies support the efficacy of lactoferrin [Andreu, Cutone, Mirabelli, Ostrov, Piacentini, Salaris, Yazawa].
Yazawa et al., 31 Mar 2023, preprint, 8 authors.
Contact: toyamaeiken3@juno.ocn.ne.jp.
In Vitro studies are an important part of preclinical research, however results may be very different in vivo.
All Studies   Meta Analysis   Submit Updates or Corrections
This PaperLactoferrinAll
Evaluation of SARS-CoV-2 isolation in cell culture from nasal/nasopharyngeal swabs or saliva specimens of patients with COVID-19
Shunsuke Yazawa, Emiko Yamazaki, Yumiko Saga, Masae Itamochi, Noriko Inasaki, Takahisa Shimada, Kazunori Oishi, Hideki Tani
doi:10.21203/rs.3.rs-2676422/v1
It has been revealed that SARS-CoV-2 can be e ciently isolated from clinical specimens such as nasal/nasopharyngeal swabs or saliva in cultured cells. In this study, we examined the e ciency of viral isolation including SARS-CoV-2 mutant strains between nasal/nasopharyngeal swab or saliva specimens. Furthermore, we also examined the comparison of viral isolation rates by sample species using simulated specimens for COVID-19. As a result, it was found that the isolation e ciency of SARS-CoV-2 in the saliva specimens was signi cantly lower than that in the nasal/nasopharyngeal swab specimens. In order to determine which component of saliva is responsible for the lower isolation rate of saliva specimens, we tested the abilities of lactoferrin, amylase, cathelicidin, and mucin, which are considered to be abundant in saliva, to inhibit the infection of SARS-CoV-2 pseudotyped viruses (SARS-CoV-2pv). Lactoferrin and amylase were found to inhibit SARS-CoV-2pv infection. In conclusion, even if the same number of viral genome copies was detected by the real-time RT-PCR test, infection of SARS-CoV-2 present in saliva is thought to be inhibited by inhibitory factors such as lactoferrin and amylase, compared to nasal/nasopharyngeal swab specimens.
Declarations Ethical approval This study was performed in accordance with the Helsinki Declaration and was approved by the ethical review board of the Toyama Institute of Health (approval No.: R2-1). The need of informed consent was also waived by the ethical review board of the Toyama Institute of Health (approval No.: R2-1). Con ict of interest We have no potential con icts of interest in relation to this work.
References
Dale, Localized antimicrobial peptide expression in human gingiva, J Periodontal Res, doi:10.1034/j.1600-0765.2001.360503.x
Griesemer, Evaluation of Specimen Types and Saliva Stabilization Solutions for SARS-CoV-2 Testing, J Clin Microbiol, doi:10.1128/JCM.01418-20
Hu, Meng, Zhang, Xiang, Wang, The in vitro antiviral activity of lactoferrin against common human coronaviruses and SARS-CoV-2 is mediated by targeting the heparan sulfate coreceptor, Emerg Microbes Infect, doi:10.1080/22221751.2021.1888660
Igarashi, Viral isolation analysis of SARS-CoV-2 from clinical specimens of COVID-19 patients, J Infect Chemother, doi:10.1016/j.jiac.2021.10.028
Kim, Duration of Culturable SARS-CoV-2 in Hospitalized Patients with Covid-19, N Engl J Med, doi:10.1056/NEJMc2027040
Kobayashi-Sakamoto, Bovine lactoferrin increases the poly(I:C)-induced antiviral response in vitro, Biochem Cell Biol, doi:10.1139/bcb-2021-0342
Lai, Identi ed human breast milk compositions effectively inhibit SARS-CoV-2 and variants infection and replication, iScience, doi:10.1016/j.isci.2022.104136
Lynge Pedersen, Belstrom, The role of natural salivary defences in maintaining a healthy oral microbiota, J Dent, doi:10.1016/j.jdent.2018.08.010
Matsuyama, Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells, Proc Natl Acad Sci U S A, doi:10.1073/pnas.2002589117
Scola, Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards, Eur J Clin Microbiol Infect Dis, doi:10.1007/s10096-020-03913-9
Singanayagam, Duration of infectiousness and correlation with RT-PCR cycle threshold values in cases of COVID-19, England, January to, Euro Surveill, doi:10.2807/1560-7917.ES.2020.25.32.2001483
Suzuki, Attenuated fusogenicity and pathogenicity of SARS-CoV-2 Omicron variant, Nature, doi:10.1038/s41586-022-04462-1
Tani, Evaluation of SARS-CoV-2 neutralizing antibodies using a vesicular stomatitis virus possessing SARS-CoV-2 spike protein, Virol J, doi:10.1186/s12985-021-01490-7
Tenovuo, Lehtonen, Aaltonen, Vilja, Tuohimaa, Antimicrobial factors in whole saliva of human infants, Infect Immun, doi:10.1128/iai.51.1.49-53.1986
Yamaguchi, Deguchi, Miyazaki, The effects of exercise in forest and urban environments on sympathetic nervous activity of normal young adults, J Int Med Res, doi:10.1177/147323000603400204
Young, Viral Dynamics and Immune Correlates of Coronavirus Disease 2019 (COVID-19) Severity, Clin Infect Dis, doi:10.1093/cid/ciaa1280
Zhang, An updated review of SARS-CoV-2 detection methods in the context of a novel coronavirus pandemic, Bioeng Transl Med, doi:10.1002/btm2.10356
Loading..
Please send us corrections, updates, or comments. Vaccines and treatments are complementary. All practical, effective, and safe means should be used based on risk/benefit analysis. No treatment, vaccine, or intervention is 100% available and effective for all current and future variants. We do not provide medical advice. Before taking any medication, consult a qualified physician who can provide personalized advice and details of risks and benefits based on your medical history and situation. FLCCC and WCH provide treatment protocols.
  or use drag and drop   
Submit