Efficacy of the Sentinox Spray in Reducing Viral Load in Mild COVID-19 and Its Virucidal Activity against Other Respiratory Viruses: Results of a Randomized Controlled Trial and an In Vitro Study

DOI record: { "DOI": "10.3390/v14051033", "ISSN": [ "1999-4915" ], "URL": "http://dx.doi.org/10.3390/v14051033", "abstract": "<jats:p>Sentinox (STX) is an acid-oxidizing solution containing hypochlorous acid in spray whose virucidal activity against SARS-CoV-2 has been demonstrated. In this paper, results of a randomized controlled trial (RCT) on the efficacy of STX in reducing viral load in mild COVID-19 patients (NCT04909996) and a complementary in vitro study on its activity against different respiratory viruses are reported. In the RCT, 57 patients were randomized (1:1:1) to receive STX three (STX-3) or five (STX-5) times/day plus standard therapy or standard therapy only (controls). Compared with controls, the log10 load reduction in groups STX-3 and STX-5 was 1.02 (p = 0.14) and 0.18 (p = 0.80), respectively. These results were likely driven by outliers with extreme baseline viral loads. When considering subjects with baseline cycle threshold values of 20–30, STX-3 showed a significant (p = 0.016) 2.01 log10 reduction. The proportion of subjects that turned negative by the end of treatment (day 5) was significantly higher in the STX-3 group than in controls, suggesting a shorter virus clearance time. STX was safe and well-tolerated. In the in vitro study, ≥99.9% reduction in titers against common respiratory viruses was observed. STX is a safe device with large virucidal spectrum and may reduce viral loads in mild COVID-19 patients.</jats:p>", "alternative-id": [ "v14051033" ], "author": [ { "ORCID": "https://orcid.org/0000-0002-2677-0551", "affiliation": [ { "name": "Department of Health Sciences (DISSAL), University of Genoa, 16132 Genoa, Italy" } ], "authenticated-orcid": false, "family": "Panatto", "given": "Donatella", "sequence": "first" }, { "ORCID": "https://orcid.org/0000-0002-2433-9610", "affiliation": [ { "name": "Department of Health Sciences (DISSAL), University of Genoa, 16132 Genoa, Italy" }, { "name": "Hygiene Unit, San Martino Policlinico Hospital-IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy" } ], "authenticated-orcid": false, "family": "Orsi", "given": "Andrea", "sequence": "additional" }, { "affiliation": [ { "name": "Hygiene Unit, San Martino Policlinico Hospital-IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy" } ], "family": "Bruzzone", "given": "Bianca", "sequence": "additional" }, { "affiliation": [ { "name": "Hygiene Unit, San Martino Policlinico Hospital-IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy" } ], "family": "Ricucci", "given": "Valentina", "sequence": "additional" }, { "affiliation": [ { "name": "NG Scientific Consulting, 20091 Bresso, Italy" } ], "family": "Fedele", "given": "Guido", "sequence": "additional" }, { "affiliation": [ { "name": "APR Applied Pharma Research SA, via Corti 5, CH-6828 Balerna, Switzerland" } ], "family": "Reiner", "given": "Giorgio", "sequence": "additional" }, { "ORCID": "https://orcid.org/0000-0001-9154-6057", "affiliation": [ { "name": "APR Applied Pharma Research SA, via Corti 5, CH-6828 Balerna, Switzerland" } ], "authenticated-orcid": false, "family": "Giarratana", "given": "Nadia", "sequence": "additional" }, { "ORCID": "https://orcid.org/0000-0001-9608-8892", "affiliation": [ { "name": "Hygiene Unit, San Martino Policlinico Hospital-IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy" } ], "authenticated-orcid": false, "family": "Domnich", "given": "Alexander", "sequence": "additional" }, { "ORCID": "https://orcid.org/0000-0002-8463-8487", "affiliation": [ { "name": "Department of Health Sciences (DISSAL), University of Genoa, 16132 Genoa, Italy" }, { "name": "Hygiene Unit, San Martino Policlinico Hospital-IRCCS for Oncology and Neurosciences, 16132 Genoa, Italy" } ], "authenticated-orcid": false, "family": "Icardi", "given": "Giancarlo", "sequence": "additional" }, { "affiliation": [], "name": "STX Study Group", "sequence": "additional" } ], "container-title": "Viruses", "container-title-short": "Viruses", "content-domain": { "crossmark-restriction": false, "domain": [] }, "created": { "date-parts": [ [ 2022, 5, 15 ] ], "date-time": "2022-05-15T13:48:22Z", "timestamp": 1652622502000 }, "deposited": { "date-parts": [ [ 2024, 7, 29 ] ], "date-time": "2024-07-29T15:09:41Z", "timestamp": 1722265781000 }, "funder": [ { "name": "APR, a subsidiary of RELIEF THERAPEUTICS Holding SA, Switzerland" } ], "indexed": { "date-parts": [ [ 2025, 2, 21 ] ], "date-time": "2025-02-21T16:37:43Z", "timestamp": 1740155863477, "version": "3.37.3" }, "is-referenced-by-count": 4, "issue": "5", "issued": { "date-parts": [ [ 2022, 5, 12 ] ] }, "journal-issue": { "issue": "5", "published-online": { "date-parts": [ [ 2022, 5 ] ] } }, "language": "en", "license": [ { "URL": "https://creativecommons.org/licenses/by/4.0/", "content-version": "vor", "delay-in-days": 0, "start": { "date-parts": [ [ 2022, 5, 12 ] ], "date-time": "2022-05-12T00:00:00Z", "timestamp": 1652313600000 } } ], "link": [ { "URL": "https://www.mdpi.com/1999-4915/14/5/1033/pdf", "content-type": "unspecified", "content-version": "vor", "intended-application": "similarity-checking" } ], "member": "1968", "original-title": [], "page": "1033", "prefix": "10.3390", "published": { "date-parts": [ [ 2022, 5, 12 ] ] }, "published-online": { "date-parts": [ [ 2022, 5, 12 ] ] }, "publisher": "MDPI AG", "reference": [ { "key": "ref_1", "unstructured": "World Health Organization (WHO) (2022, May 09). 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