In Vitro Determination of Inhibitory Effects of Humic Substances Complexing Zn and Se on SARS-CoV-2 Virus Replication
Polett Hajdrik, Bernadett Pályi, Zoltán Kis, Noémi Kovács, Dániel Sándor Veres, Krisztián Szigeti, Ferenc Budán, Imre Hegedüs, Tibor Kovács, Ralf Bergmann, Domokos Máthé
Foods, doi:10.3390/foods11050694
1) Background: Humic substances are well-known human nutritional supplement materials and they play an important performance-enhancing role as animal feed additives. For decades, ingredients of humic substances have been proven to carry potent antiviral effects against different viruses. (2) Methods: Here, the antiviral activity of a humic substance containing ascorbic acid, Se − and Zn 2+ ions intended as a nutritional supplement material was investigated against SARS-CoV-2 virus B1.1.7 Variant of Concern ("Alpha Variant") in a VeroE6 cell line. (3) Results: This combination has a significant in vitro antiviral effect at a very low concentration range of its intended active ingredients. (4) Conclusions: Even picomolar concentration ranges of humic substances, Vitamin C and Zn/Se ions in the given composition, were enough to achieve 50% viral replication inhibition in the applied SARS-CoV-2 virus inhibition test.
Conflicts of Interest: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
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'abstract': '<jats:p>(1) Background: Humic substances are well-known human nutritional supplement '
'materials and they play an important performance-enhancing role as animal feed additives. For '
'decades, ingredients of humic substances have been proven to carry potent antiviral effects '
'against different viruses. (2) Methods: Here, the antiviral activity of a humic substance '
'containing ascorbic acid, Se− and Zn2+ ions intended as a nutritional supplement material was '
'investigated against SARS-CoV-2 virus B1.1.7 Variant of Concern (“Alpha Variant”) in a VeroE6 '
'cell line. (3) Results: This combination has a significant in vitro antiviral effect at a '
'very low concentration range of its intended active ingredients. (4) Conclusions: Even '
'picomolar concentration ranges of humic substances, Vitamin C and Zn/Se ions in the given '
'composition, were enough to achieve 50% viral replication inhibition in the applied '
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