In Vitro Determination of Inhibitory Effects of Humic Substances Complexing Zn and Se on SARS-CoV-2 Virus Replication
Hajdrik et al.,
In Vitro Determination of Inhibitory Effects of Humic Substances Complexing Zn and Se on SARS-CoV-2 Virus..,
Foods, doi:10.3390/foods11050694 (In Vitro)
In Vitro study of a humic substance containing vitamin C, selemium ions, and zinc ions, showing 50% SARS-CoV-2 inhibition at picomolar concentrations.
2 In Vitro studies support the efficacy of zinc
[Hajdrik, Panchariya].
Hajdrik et al., 26 Feb 2022, peer-reviewed, 11 authors.
Contact:
domokos.mathe@hcemm.eu (corresponding author), h.polett0809@gmail.com, kovacsnoi@hotmail.com, veres.daniel@med.semmelweis-univ.hu, krisztian.szigeti@gmail.com, hegedus.imre1@med.semmelweis-univ.hu, rkbergmann@web.de, palyi.bernadett@nnk.gov.hu, kis.zoltan@nnk.gov.hu, budfer2@gmail.com, kovacstiborjanos@gmail.com.
In Vitro studies are an important part of preclinical research, however results may be very different in vivo.
Abstract: foods
Article
In Vitro Determination of Inhibitory Effects of Humic Substances
Complexing Zn and Se on SARS-CoV-2 Virus Replication
Polett Hajdrik 1,† , Bernadett Pályi 2,† , Zoltán Kis 2,3 , Noémi Kovács 1,4 , Dániel Sándor Veres 1 , Krisztián Szigeti 1 ,
Ferenc Budán 5,6 , Imre Hegedüs 1 , Tibor Kovács 7 , Ralf Bergmann 1,8 and Domokos Máthé 1,4,9, *
1
2
3
4
5
6
7
8
9
Citation: Hajdrik, P.; Pályi, B.; Kis, Z.;
*
†
Department of Biophysics and Radiation Biology, Semmelweis University, Üllői út 26., H-1085 Budapest,
Hungary; h.polett0809@gmail.com (P.H.); kovacsnoi@hotmail.com (N.K.);
veres.daniel@med.semmelweis-univ.hu (D.S.V.); krisztian.szigeti@gmail.com (K.S.);
hegedus.imre1@med.semmelweis-univ.hu (I.H.); rkbergmann@web.de (R.B.)
National Biosafety Laboratory, National Public Health Center, Albert Flórián út 2-6, H-1097 Budapest,
Hungary; palyi.bernadett@nnk.gov.hu (B.P.); kis.zoltan@nnk.gov.hu (Z.K.)
Department of Microbiology, Semmelweis University, Üllői út 26., H-1085 Budapest, Hungary
CROmed Translational Research Ltd., Tűzoltó u. 37-47, H-1094 Budapest, Hungary
Institute of Transdisciplinary Discoveries, Medical School, University of Pécs, Ifjúság útja 11, H-7624 Pécs,
Hungary; budfer2@gmail.com
Institute of Physiology, Medical School, University of Pécs, Szigeti út 12, H-7624 Pécs, Hungary
Institute of Radiochemistry and Radioecology, University of Pannonia, Egyetem u. 10., H-8200 Veszprem,
Hungary; kovacstiborjanos@gmail.com
Helmholtz-Zentrum Dresden Rossendorf, Bautzner Landstraße 400, 01328 Dresden, Germany
Hungarian Centre of Excellence for Molecular Medicine, In Vivo Imaging Advanced Core Facility,
Semmelweis University Site, Római Blvd. 21, H-6723 Szeged, Hungary
Correspondence: domokos.mathe@hcemm.eu
These authors contributed equally to this work.
Kovács, N.; Veres, D.S.; Szigeti, K.;
Budán, F.; Hegedüs, I.; Kovács, T.;
Bergmann, R.; et al. In Vitro
Determination of Inhibitory Effects of
Humic Substances Complexing Zn
and Se on SARS-CoV-2 Virus
Replication. Foods 2022, 11, 694.
https://doi.org/10.3390/
foods11050694
Academic Editors: Maria Luisa
Serralheiro and Rita Isabel Pacheco
Received: 31 January 2022
Accepted: 23 February 2022
Published: 26 February 2022
Abstract: (1) Background: Humic substances are well-known human nutritional supplement materials and they play an important performance-enhancing role as animal feed additives. For decades,
ingredients of humic substances have been proven to carry potent antiviral effects against different
viruses. (2) Methods: Here, the antiviral activity of a humic substance containing ascorbic acid, Se−
and Zn2+ ions intended as a nutritional supplement material was investigated against SARS-CoV-2
virus B1.1.7 Variant of Concern (“Alpha Variant”) in a VeroE6 cell line. (3) Results: This combination
has a significant in vitro antiviral effect at a very low concentration range of its intended active
ingredients. (4) Conclusions: Even picomolar concentration ranges of humic substances, Vitamin C
and Zn/Se ions in the given composition, were enough to achieve 50% viral replication inhibition in
the applied SARS-CoV-2 virus inhibition test.
Keywords: SARS-CoV-2; humic acid; fulvic acid; Zn-Se-ascorbic acid complex; antiviral activity;
RT-PCR
Publisher’s Note: MDPI stays neutral
with regard to jurisdictional claims in
published maps and institutional affil-
Please send us corrections, updates, or comments. Vaccines and
treatments are complementary. All practical, effective, and safe means should
be used based on risk/benefit analysis. No treatment, vaccine, or intervention
is 100% available and effective for all current and future variants. We do not
provide medical advice. Before taking any medication, consult a qualified
physician who can provide personalized advice and details of risks and
benefits based on your medical history and situation.
FLCCC and
WCH
provide treatment protocols.
Submit