Effect of oral antiseptics on the viral load of SARS-CoV-2: A randomized controlled trial
Sevinç Gül et al.
, Effect of oral antiseptics on the viral load of SARS-CoV-2: A randomized controlled trial
, Dental and Medical Problems, doi:10.17219/dmp/150831
RCT with 21 PVP-I and 20 saline patients gargling for 30 seconds and testing PCR Ct after 30 minutes, showing greater improvement with PVP-I, without statistical significance.
Ct values differ across testing platforms, however the reported Ct value difference can represent a large difference in viral load. For example, using the calibration included with the ct2vl converter, the reported difference in mean Ct values corresponds to a reduction in viral load of over 3x for PVP-I.
risk of viral load, 99.5% lower, RR 0.005, p = 0.37, treatment mean 1.85 (±7.06) n=21, control mean 0.01 (±5.89) n=20, relative improvement in Ct value.
Effect extraction follows pre-specified rules prioritizing more serious outcomes. Submit updates
Sevinç Gül et al., 29 Jul 2022, Randomized Controlled Trial, Turkey, peer-reviewed, 4 authors, this trial compares with another treatment - results may be better when compared to placebo.
Abstract: Original paper
Effect of oral antiseptics on the viral load of SARS-CoV-2:
A randomized controlled trial
Sema Nur Sevinç Gül1,A,B,D,F, Alparslan Dilsiz1,A,E, İmran Sağlık2,C,E, Nurten Nur Aydın3,B,C
Department of Periodontology, Faculty of Dentistry, Atatürk University, Erzurum, Turkey
Department of Medical Microbiology, Faculty of Medicine, Bursa Uludağ University, Turkey
Department of Infectious Diseases and Clinical Microbiology, Erzurum Regional Training and Research Hospital, Turkey
A – research concept and design; B – collection and/or assembly of data; C – data analysis and interpretation;
D – writing the article; E – critical revision of the article; F – final approval of the article
Dental and Medical Problems, ISSN 1644-387X (print), ISSN 2300-9020 (online)
Address for correspondence
Sema Nur Sevinç Gül
Conflict of interest
We would like to thank Suphi Özçomak for statistical assistance
and Bioeksen R&D Technologies for providing RT-PCR tests.
Received on April 15, 2022
Reviewed on May 31, 2022
Accepted on June 9, 2022
Published online on July 29, 2022
Dent Med Probl.
Background. In the oral cavity, which plays an important role in the transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is possible to reduce the viral load of SARS-CoV-2 with
antiseptics, thereby minimizing the transmission of the virus during dental procedures.
Objectives. The aim of this study was to clinically evaluate the effect of the hypochlorous acid (HClO) and
povidone-iodine (PVP-I) solutions on the oral viral load of SARS-CoV-2.
Material and methods. This randomized controlled trial was conducted on 75 patients hospitalized in
the COVID-19 ward of a local hospital. All the patients included in the study were within the first 24 h
of hospitalization and the first 5 days of coronavirus disease 2019 (COVID-19) symptoms. The viral load
of mouthwash samples was measured with the cycle threshold (Ct) value of SARS-CoV-2 through a realtime reverse transcription polymerase chain reaction (RT-PCR). The patients were divided into 3 groups.
The effect on the patient’s SARS-CoV-2 viral load was investigated after gargling the mouths and throats
for 30 s with HClO, PVP-I and isotonic saline. First, a sample was taken after gargling with isotonic saline,
then another sample was taken after gargling for 30 s with a particular antiseptic to determine the viral
load of SARS-CoV-2.
Results. Comparing the before and after mouthwash samples from all 3 groups, there were no statistically
significant differences in the Ct values before and after gargling (p > 0.05). However, there were statistically significant differences in the number of negative samples after the use of HClO and PVP-I, which were
positive before gargling (p < 0.05).
Sevinç Gül SN, Dilsiz A, Sağlık İ, Aydın NN. Effect of oral antiseptics
on the viral load of SARS-CoV-2: A randomized controlled trial
[published online as ahead of print on July 29, 2022]. Dent Med
Copyright by Author(s)
This is an article distributed under the terms of the
Creative Commons Attribution 3.0 Unported License (CC BY 3.0)
Conclusions. In the light of the data obtained in this study, there is insufficient evidence that gargling with
HClO or PVP-I reduces..
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