Povidone-Iodine Demonstrates Rapid In Vitro Virucidal Activity Against SARS-CoV-2, The Virus Causing COVID-19 Disease
Danielle E Anderson, Velraj Sivalingam, Adrian Eng Zheng Kang, Abhishek Ananthanarayanan, Harsha Arumugam, Timothy M Jenkins, Yacine Hadjiat, Maren Eggers
Infectious Diseases and Therapy, doi:10.1007/s40121-020-00316-3
Introduction: As of 22 June 2020, Severe Acute Respiratory Syndrome (SARS)-coronavirus (CoV)-2 has infected more than 8.95 million people worldwide, causing [ 468,000 deaths. The virus is transmitted through respiratory droplets and physical contact from contaminated surfaces to the mucosa. Hand hygiene and oral decontamination among other measures are key to preventing the spread of the virus. We report the in vitro virucidal activity of topical and oral povidone-iodine (PVP-I) products against SARS-CoV-2. Methods: Suspension assays were used to assess the virucidal activity of PVP-I against SARS-CoV-2. Products were tested at a contact time of 30 s for virucidal activity. Viral titres were calculated using the Spearman-Ka ¨rber method and reported as median tissue culture infectious dose (TCID 50 )/mL. Results: All four products [antiseptic solution (PVP-I 10%), skin cleanser (PVP-I 7.5%), gargle and mouth wash (PVP-I 1%) and throat spray (PVP-I 0.45%)] achieved C 99.99% virucidal activity against SARS-CoV-2, corresponding to C 4 log 10 reduction of virus titre, within 30 s of contact.
Conclusion: This study provides evidence of rapid and effective virucidal activity of PVP-I against SARS-CoV-2. PVP-I-based products are widely available for medical and personal use for hand hygiene and oral decontamination, and could be readily integrated into coronavirus disease, COVID-19, infection control measures in hospital and community settings.
References
Bai, Yao, Wei, Presumed asymptomatic carrier of COVID-19, JAMA
Cascella, Rajnik, Cuomo, Dulebohn, Napoli, Features, evaluation and treatment coronavirus (COVID-19)
Chin, Chu, Perera, Stability of SARS-CoV-2 in different environmental conditions, Lancet Microbe
Comite ´europe ´en, ? A1:2015: Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of virucidal activity in the medical area. Test method and requirements
Corman, Muth, Niemeyer, Drosten, Hosts and sources of endemic human coronaviruses, Adv Virus Res
Eggers, Eickmann, Zorn, Rapid and effective virucidal activity of povidone-iodine products against middle east respiratory syndrome coronavirus (MERS-CoV) and modified vaccinia virus Ankara (MVA), Infect Dis Ther
Eggers, Koburger-Janssen, Eickmann, Zorn, In vitro bactericidal and virucidal efficacy of povidone-iodine gargle/mouthwash against respiratory and oral tract pathogens, Infect Dis Ther
Eggers, Koburger-Janssen, Ward, Newby, Mu ¨ller S, Bactericidal and virucidal activity of povidone-iodine and chlorhexidine gluconate cleansers in an in vivo hand hygiene clinical simulation study, Infect Dis Ther
Gui, Pepe, Magalini, Just one more hygiene practice in COVID-19, Eur Rev Med Pharmacol Sci
Kariwa, Fujii, Takashima, Inactivation of SARS coronavirus by means of povidone-iodine, physical conditions and chemical reagents, Dermatology
Kawana, Kitamura, Nakagomi, Inactivation of human viruses by povidone-iodine in comparison with other antiseptics, Dermatology
Kirk-Bayley, Challacombe, Sunkaraneni, Combes, The use of povidone iodine nasal spray and mouthwash during the current COVID-19 pandemic may reduce cross infection and protect healthcare workers,
doi:10.2139/ssrn.3563092Mady, Kubik, Baddour, Snyderman, Rowan, Consideration of povidone-iodine as a public health intervention for COVID-19: Utilization as ''Personal Protective Equipment'' for frontline providers exposed in high-risk head and neck and skull base oncology care, Oral Oncol
Nagatake, Ahmed, Oishi, Prevention of respiratory lnfections by povidone-lodine gargle, Dermatology
O'donnell, Thomas, Stanton, Potential role of oral rinses targeting the viral lipid envelope in SARS-CoV-2 infection, Function
Sanche, Lin, Xu, Romero-Severson, Hengartner et al., High contagiousness and rapid spread of severe acute respiratory syndrome coronavirus 2, Emerg Infect Dis
Spearman, The method of 'right and wrong cases' ('constant stimuli') without Gauss's formulae, Br J Psychol
To, Tsang, Leung, Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study, Lancet Infect Dis
Vogt, Hauser, Mueller, Bosse, Hopp, Efficacy of conventional and liposomal povidoneiodine in infected mesh skin grafts: an exploratory study, Infect Dis Ther
Wo ¨lfel, Corman, Guggemos, Virological assessment of hospitalized patients with COVID-2019, Nature
Zhao, Lin, Ran, Preliminary estimation of the basic reproduction number of novel coronavirus (2019-nCoV) in China, from 2019 to 2020: a data-driven analysis in the early phase of the outbreak, Int J Infect Dis
Zhu, Zhang, Wang, A novel coronavirus from patients with pneumonia in China, 2019, N Engl J Med
¨rber, Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche, Archiv Exper Pathol Pharmakol
