Randomized clinical trial to assess the impact of oral intervention with cetylpyridinium chloride to reduce salivary SARS‐CoV‐2 viral load
Rosa Tarragó‐gil, María José Gil‐mosteo, Mercedes Aza‐pascual‐salcedo, María Jesús Lallana Alvarez, Raquel Refusta Ainaga, Natalia Lázaro Gimeno, Roberto Fuentes Viñuales, Yolanda Millán Fernández, Jesica Montero Marco, Elena Altarribas Bolsa, Jessica Bueno Sancho, Sonia Algarate Cajo, Daniel Perez‐zsolt, Dàlia Raïch‐regué, Jordana Muñoz‐basagoiti, Nuria Izquierdo‐useros, Vanessa Blanc Pociello, Rubén León, Diana Serrano Peris
Journal of Clinical Periodontology, doi:10.1111/jcpe.13746
Aim: Aerosols released from the oral cavity help spread the SARS-CoV-2 virus. The use of a mouthwash formulated with an antiviral agent could reduce the viral load in saliva, helping to lower the spread of the virus. The aim of this study was to assess the efficacy of a mouthwash with 0.07% cetylpyridinium chloride (CPC) to reduce the viral load in the saliva of Coronavirus disease 2019 (COVID-19) patients.
Materials and Methods: In this multi-centre, single-blind, randomized, parallel group clinical trial, 80 COVID-19 patients were enrolled and randomized to two groups, namely test (n = 40) and placebo (n = 40). Saliva samples were collected at baseline and 2 h after rinsing. The samples were analysed by reverse transcriptionquantitative polymerase chain reaction (RT-qPCR) and an enzyme-linked immunosorbent assay test specific for the nucleocapsid (N) protein of SARS-CoV-2. Results: With RT-qPCR, no significant differences were observed between the placebo group and the test group. However, 2 h after a single rinse, N protein concentration in saliva was significantly higher in the test group, indicating an increase in lysed virus.
Conclusions: The use of 0.07% CPC mouthwash induced a significant increase in N protein detection in the saliva of COVID-19 patients. Lysis of the virus in the mouth could help reduce the transmission of SARS-CoV-2. However, more studies are required to prove this.
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'abstract': '<jats:title>Abstract</jats:title><jats:sec><jats:title>Aim</jats:title><jats:p>Aerosols '
'released from the oral cavity help spread the SARS‐CoV‐2 virus. The use of a mouthwash '
'formulated with an antiviral agent could reduce the viral load in saliva, helping to lower '
'the spread of the virus. The aim of this study was to assess the efficacy of a mouthwash with '
'0.07% cetylpyridinium chloride (CPC) to reduce the viral load in the saliva of Coronavirus '
'disease 2019 (COVID‐19) patients.</jats:p></jats:sec><jats:sec><jats:title>Materials and '
'Methods</jats:title><jats:p>In this multi‐centre, single‐blind, randomized, parallel group '
'clinical trial, 80 COVID‐19 patients were enrolled and randomized to two groups, namely test '
'(<jats:italic>n</jats:italic>\xa0=\xa040) and placebo (<jats:italic>n</jats:italic>\xa0=\xa0'
'40). Saliva samples were collected at baseline and 2\xa0h after rinsing. The samples were '
'analysed by reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) and an '
'enzyme‐linked immunosorbent assay test specific for the nucleocapsid (N) protein of '
'SARS‐CoV‐2.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>With '
'RT‐qPCR, no significant differences were observed between the placebo group and the test '
'group. However, 2\xa0h after a single rinse, N protein concentration in saliva was '
'significantly higher in the test group, indicating an increase in lysed '
'virus.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>The use of '
'0.07% CPC mouthwash induced a significant increase in N protein detection in the saliva of '
'COVID‐19 patients. Lysis of the virus in the mouth could help reduce the transmission of '
'SARS‐CoV‐2. However, more studies are required to prove this.</jats:p></jats:sec>',
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