Antiviral Effect of Melatonin on Caco-2 Cell Organoid Culture: Trick or Treat?

{ 'indexed': { 'date-parts': [[2024, 11, 6]], 'date-time': '2024-11-06T05:24:19Z', 'timestamp': 1730870659820, 'version': '3.28.0'}, 'reference-count': 31, 'publisher': 'MDPI AG', 'issue': '22', 'license': [ { 'start': { 'date-parts': [[2024, 11, 5]], 'date-time': '2024-11-05T00:00:00Z', 'timestamp': 1730764800000}, 'content-version': 'vor', 'delay-in-days': 0, 'URL': 'https://creativecommons.org/licenses/by/4.0/'}], 'funder': [ {'name': 'Research Council of Lithuania', 'award': ['P-MIP-23-116', 'S-MIP-23-110']}, {'name': 'Research Fund of the Lithuanian University of Health Sciences'}], 'content-domain': {'domain': [], 'crossmark-restriction': False}, 'abstract': '<jats:p>Melatonin is a hormone naturally produced by the body that has recently been found to ' 'have antiviral properties. However, its antiviral mechanisms are not entirely understood. ' 'Using Caco-2 cells, we developed a gastrointestinal organoid model to investigate the impact ' 'of melatonin on cellular organoid culture response to Poly I:C-induced viral inflammation in ' 'the gastrointestinal tract. Melatonin was found to have different effect when applied as a ' 'pretreatment before the induction of viral inflammation or as a treatment after it. Melatonin ' 'pretreatment after Poly I:C stimulation did not protect organoids from size reduction but ' 'enhanced cell proliferation, especially when lower (1 and 10 µM) melatonin concentrations ' 'were used. On the other hand, treatment with melatonin after the induction of viral ' 'inflammation helped to maintain the size of the organoids while reducing cell proliferation. ' 'In pretreated cells, reduced IFNLR1 expression was found, while melatonin treatment increased ' 'IFNLR1 expression and reduced the production of viral cytokines, such as IFNλ1 and STAT1-3, ' 'but did not prevent from apoptosis. The findings of this study emphasize the importance of ' 'type III IFNs in antiviral defense in epithelial gastrointestinal cells and shed more light ' 'on the antiviral properties of melatonin as a potential therapeutic substance.</jats:p>', 'DOI': '10.3390/ijms252211872', 'type': 'journal-article', 'created': {'date-parts': [[2024, 11, 5]], 'date-time': '2024-11-05T10:30:53Z', 'timestamp': 1730802653000}, 'page': '11872', 'source': 'Crossref', 'is-referenced-by-count': 0, 'title': 'Antiviral Effect of Melatonin on Caco-2 Cell Organoid Culture: Trick or Treat?', 'prefix': '10.3390', 'volume': '25', 'author': [ { 'given': 'Milda', 'family': 'Šeškutė', 'sequence': 'first', 'affiliation': [ { 'name': 'Department of Pediatrics, Lithuanian University of Health ' 'Sciences, 44307 Kaunas, Lithuania'}, { 'name': 'Institute of Physiology and Pharmacology, Lithuanian University ' 'of Health Sciences, 44307 Kaunas, Lithuania'}]}, { 'given': 'Dominyka', 'family': 'Žukaitė', 'sequence': 'additional', 'affiliation': [ { 'name': 'Faculty of Medicine, Lithuanian University of Health Sciences, ' '44307 Kaunas, Lithuania'}]}, { 'ORCID': 'http://orcid.org/0000-0003-3182-9754', 'authenticated-orcid': False, 'given': 'Goda', 'family': 'Laucaitytė', 'sequence': 'additional', 'affiliation': [ { 'name': 'Department of Pediatrics, Lithuanian University of Health ' 'Sciences, 44307 Kaunas, Lithuania'}, { 'name': 'Institute of Physiology and Pharmacology, Lithuanian University ' 'of Health Sciences, 44307 Kaunas, Lithuania'}]}, { 'ORCID': 'http://orcid.org/0000-0002-5806-6155', 'authenticated-orcid': False, 'given': 'Rūta', 'family': 'Inčiūraitė', 'sequence': 'additional', 'affiliation': [ { 'name': 'Institute for Digestive Research, Lithuanian University of ' 'Health Sciences, 44307 Kaunas, Lithuania'}]}, { 'given': 'Mantas', 'family': 'Malinauskas', 'sequence': 'additional', 'affiliation': [ { 'name': 'Institute of Physiology and Pharmacology, Lithuanian University ' 'of Health Sciences, 44307 Kaunas, Lithuania'}]}, { 'ORCID': 'http://orcid.org/0000-0002-6997-4917', 'authenticated-orcid': False, 'given': 'Lina', 'family': 'Jankauskaitė', 'sequence': 'additional', 'affiliation': [ { 'name': 'Department of Pediatrics, Lithuanian University of Health ' 'Sciences, 44307 Kaunas, Lithuania'}, { 'name': 'Institute of Physiology and Pharmacology, Lithuanian University ' 'of Health Sciences, 44307 Kaunas, Lithuania'}]}], 'member': '1968', 'published-online': {'date-parts': [[2024, 11, 5]]}, 'reference': [ { 'key': 'ref_1', 'doi-asserted-by': 'crossref', 'unstructured': 'Santos-Ferreira, N., Van Dycke, J., Neyts, J., and Rocha-Pereira, J. 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