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'issue': '11',
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'abstract': '<jats:p>SARS-CoV-2, the causative virus for the COVID-19 disease, uses its spike glycoprotein '
'to bind to human ACE2 as a first step for viral entry into the cell. For this reason, great '
'efforts have been made to find mechanisms that disrupt this interaction, avoiding the '
'infection. Nitric oxide (NO) is a soluble endogenous gas with known antiviral and '
'immunomodulatory properties. In this study, we aimed to test whether NO could inhibit the '
'binding of the viral spike to ACE2 in human cells and its effects on ACE2 enzymatic activity. '
'Our results show that ACE2 activity was decreased by the NO donors DETA-NONOate and GSNO and '
'by the NO byproduct peroxynitrite. Furthermore, we found that DETA-NONOate could break the '
'spike–ACE2 interaction using the spike from two different variants (Alpha and Gamma) and in '
'two different human cell types. Moreover, the same result was obtained when using '
'NO-producing murine macrophages, while no significant changes were observed in ACE2 '
'expression or distribution within the cell. These results support that it is worth '
'considering NO as a therapeutic agent for COVID-19, as previous reports have '
'suggested.</jats:p>',
'DOI': '10.3390/antiox13111301',
'type': 'journal-article',
'created': { 'date-parts': [[2024, 10, 28]],
'date-time': '2024-10-28T08:15:02Z',
'timestamp': 1730103302000},
'page': '1301',
'source': 'Crossref',
'is-referenced-by-count': 0,
'title': 'Potential Beneficial Role of Nitric Oxide in SARS-CoV-2 Infection: Beyond Spike-Binding '
'Inhibition',
'prefix': '10.3390',
'volume': '13',
'author': [ { 'ORCID': 'http://orcid.org/0000-0002-3332-7603',
'authenticated-orcid': False,
'given': 'Sergio',
'family': 'Sánchez-García',
'sequence': 'first',
'affiliation': [ { 'name': 'Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, '
'Arturo Duperier 4, 28029 Madrid, Spain'}]},
{ 'given': 'Antonio',
'family': 'Castrillo',
'sequence': 'additional',
'affiliation': [ { 'name': 'Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, '
'Arturo Duperier 4, 28029 Madrid, Spain'},
{ 'name': 'Unidad de Biomedicina (Unidad Asociada al CSIC), Universidad de '
'Las Palmas de Gran Canaria, 35016 Las Palmas, Spain'}]},
{ 'ORCID': 'http://orcid.org/0000-0002-0253-5469',
'authenticated-orcid': False,
'given': 'Lisardo',
'family': 'Boscá',
'sequence': 'additional',
'affiliation': [ { 'name': 'Instituto de Investigaciones Biomédicas Sols-Morreale, CSIC-UAM, '
'Arturo Duperier 4, 28029 Madrid, Spain'},
{ 'name': 'Centro de Investigación Biomédica en Red de Enfermedades '
'Cardiovasculares (CIBERCV), Av. Monforte de Lemos 3-5, P-11, '
'28029 Madrid, Spain'}]},
{ 'ORCID': 'http://orcid.org/0000-0001-6943-7663',
'authenticated-orcid': False,
'given': 'Patricia',
'family': 'Prieto',
'sequence': 'additional',
'affiliation': [ { 'name': 'Centro de Investigación Biomédica en Red de Enfermedades '
'Cardiovasculares (CIBERCV), Av. Monforte de Lemos 3-5, P-11, '
'28029 Madrid, Spain'},
{ 'name': 'Departamento de Farmacología, Farmacognosia y Botánica, Facultad '
'de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y '
'Cajal, 28040 Madrid, Spain'}]}],
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