A Mouthwash with Cetylpyridinium Chloride is reducing salivary SARS-CoV-2 viral load in +COVID-19

{ 'indexed': {'date-parts': [[2023, 7, 28]], 'date-time': '2023-07-28T04:26:48Z', 'timestamp': 1690518408470}, 'reference-count': 30, 'publisher': 'Universidad del Valle', 'issue': '1', 'license': [ { 'start': { 'date-parts': [[2023, 6, 5]], 'date-time': '2023-06-05T00:00:00Z', 'timestamp': 1685923200000}, 'content-version': 'unspecified', 'delay-in-days': 0, 'URL': 'http://creativecommons.org/licenses/by-nc-nd/4.0'}], 'content-domain': {'domain': [], 'crossmark-restriction': False}, 'abstract': '<jats:p>Aim: The aim of this randomised, double-blind, placebo-controlled pilot clinical ' 'trial is to evaluate the capacity of a mouthwash to reduce SARS-CoV-2 viral load in the ' 'saliva of patients with COVID-19.&#x0D;\n' 'Methods: Twenty-three symptomatic SARS-CoV-2-positive outpatients were selected and ' 'randomised into two groups\xa0 and registered at NTC 04563689. Both groups rinsed and gargled ' 'for one minute with either distilled water (Placebo) or with 0.05% Cetylpyridinium chloride ' '(CPC) plus 0.12% Chlorhexidine (CHX) mouthwash (PERIOAID Intensive CareÒ). Saliva samples ' 'were collected before the use of placebo or mouthwash and after 15 minutes and 1 and 2 hours ' 'of either of the above treatment. A saliva sample was also taken five days after regular use ' 'of placebo or mouthwash twice daily. The virus was detected by qRT-PCR.&#x0D;\n' 'Results: A great heterogeneity in the viral load values was observed at baseline in both ' 'groups for nasopharyngeal and saliva samples. Most of the patients who used the mouthwash ' '(8/12) had a significant decrease in baseline viral load after 15 min (greater than 99% ' 'reduction). This inhibitory effect was maintained for up to two hours in 10 of the 12 ' 'patients. At five days, SARS-CoV-2 RNA was detected in only 1 patient from the mouthwash ' 'group and in 5 from the placebo group.&#x0D;\n' 'Conclusions: This study points out that a CPC mouthwash can reduce the viral load in saliva ' 'of COVID-positive patients. This finding may be important in transmission control of ' 'SARS-CoV-2. Nevertheless, the clinical relevance of CPC mouthwash-reduction on SARS-CoV-2 ' 'shedding in saliva requires further study</jats:p>', 'DOI': '10.25100/re.v31i1.12669', 'type': 'journal-article', 'created': {'date-parts': [[2023, 7, 27]], 'date-time': '2023-07-27T19:32:52Z', 'timestamp': 1690486372000}, 'source': 'Crossref', 'is-referenced-by-count': 0, 'title': 'A Mouthwash with Cetylpyridinium Chloride Is Reducing Salivary SARS-CoV-2 Viral Load in ' '+COVID-19.', 'prefix': '10.25100', 'volume': '31', 'author': [ {'given': 'Adolfo', 'family': 'Contreras Renjifo', 'sequence': 'first', 'affiliation': []}, { 'ORCID': 'http://orcid.org/0000-0001-6400-4906', 'authenticated-orcid': False, 'given': 'Rubén', 'family': 'León\xa0Berrios', 'sequence': 'additional', 'affiliation': []}, { 'ORCID': 'http://orcid.org/0000-0001-9006-6721', 'authenticated-orcid': False, 'given': 'Andrés', 'family': 'Castillo Giraldo', 'sequence': 'additional', 'affiliation': []}, {'given': 'José Luis', 'family': 'Bahamon', 'sequence': 'additional', 'affiliation': []}, {'given': 'Yurani', 'family': 'Giraldo', 'sequence': 'additional', 'affiliation': []}, { 'ORCID': 'http://orcid.org/0000-0003-0196-1957', 'authenticated-orcid': False, 'given': 'Gerardo Andrés', 'family': 'Libreros Zúñiga', 'sequence': 'additional', 'affiliation': []}, { 'ORCID': 'http://orcid.org/0000-0002-6530-3593', 'authenticated-orcid': False, 'given': 'Alejandro', 'family': 'Contreras Parra', 'sequence': 'additional', 'affiliation': []}, { 'ORCID': 'http://orcid.org/0000-0002-2830-8053', 'authenticated-orcid': False, 'given': 'Juan', 'family': 'Gispert', 'sequence': 'additional', 'affiliation': []}, { 'ORCID': 'http://orcid.org/0000-0002-0138-9199', 'authenticated-orcid': False, 'given': 'Julián', 'family': 'Balanta- Melo', 'sequence': 'additional', 'affiliation': []}, {'given': 'Fausto Andrés', 'family': 'Alban', 'sequence': 'additional', 'affiliation': []}, { 'ORCID': 'http://orcid.org/0000-0002-5371-7449', 'authenticated-orcid': False, 'given': 'Beatriz', 'family': 'Parra', 'sequence': 'additional', 'affiliation': []}], 'member': '10728', 'published-online': {'date-parts': [[2023, 6, 5]]}, 'reference': [ { 'key': '182094', 'unstructured': 'WHO. Coronavirus disease (COVID‐19) situation reports. (2019). ' 'https://www.who.int/emergencies/diseases/novel-coronavirus-2019/situation-reports.'}, { 'key': '182095', 'unstructured': 'Chau NVV, Thanh Lam V, Thanh Dung N, Yen LM, Minh NNQ, Hung LM, et al. ' 'The natural history and transmission potential of asymptomatic ' 'SARS-CoV-2 infection. Clin Infect Dis. 2020; 71(10), 2679–2687.'}, { 'key': '182096', 'doi-asserted-by': 'crossref', 'unstructured': 'To K, Tsang O, Yip C, Chan K, Wu T, Chan J, et al. Consistent detection ' 'of 2019 novel coronavirus in saliva. Clin Infect Dis. 2020; 71(15), ' '841–843.', 'DOI': '10.1093/cid/ciaa149'}, { 'key': '182097', 'doi-asserted-by': 'crossref', 'unstructured': 'Walsh KA, Jordan K, Clyne B, Rohde D, Drummond L, Byrne P, et al. ' 'SARS-CoV-2 detection, viral load and infectivity over the course of an ' 'infection. J Infect. 2020; 81(3): 357–371.', 'DOI': '10.1016/j.jinf.2020.06.067'}, { 'key': '182098', 'doi-asserted-by': 'crossref', 'unstructured': 'Zhu J, Guo J, Xu Y, Chen X. Viral dynamics of SARS-CoV-2 in saliva from ' 'infected patients. J Infect. 2020; 81(3): e48-e50.', 'DOI': '10.1016/j.jinf.2020.06.059'}, { 'key': '182099', 'doi-asserted-by': 'crossref', 'unstructured': 'He Z, Zhao C, Dong Q, Zhuang H, Song S, Peng G, Dwyer DE. Effects of ' 'severe acute respiratory syndrome (SARS) coronavirus infection on ' 'peripheral blood lymphocytes and their subsets. Int J Infect Dis. 2005; ' '9(6): 323-30.', 'DOI': '10.1016/j.ijid.2004.07.014'}, { 'key': '182100', 'doi-asserted-by': 'crossref', 'unstructured': 'Huang LS, Li L, Dunn L, He M. Taking account of asymptomatic infections: ' 'A modeling study of the COVID-19 outbreak on the Diamond Princess cruise ' 'ship. PLoS One. 2021; 16(3): e0248273.', 'DOI': '10.1371/journal.pone.0248273'}, { 'key': '182101', 'doi-asserted-by': 'crossref', 'unstructured': 'Ferretti L, Wymant C, Kendall M, et al. Quantifying SARS-CoV-2 ' 'transmission suggests epidemic control with digital contact tracing. ' 'Science. 2020; 368(6491): eabb6936.', 'DOI': '10.1126/science.abb6936'}, { 'key': '182102', 'doi-asserted-by': 'crossref', 'unstructured': 'Burton M, Clarkson J, Goulao B, Glenny AM, McBain A, Schilder A, et al. ' 'Antimicrobial mouthwashes (gargling) and nasal sprays administered to ' 'patients with suspected or confirmed COVID-19 infection to improve ' 'patient outcomes and to protect healthcare workers treating them. ' 'Cochrane Database Syst Rev. 2020; 9:CD013627.', 'DOI': '10.1002/14651858.CD013627.pub2'}, { 'key': '182103', 'doi-asserted-by': 'crossref', 'unstructured': 'Herrera D, Serrano J, Roldán S, Sanz M. Is the oral cavity relevant in ' 'SARS-CoV-2 pandemic? Clin Oral Investig. 2020; 24(8): 2925-2930.', 'DOI': '10.1007/s00784-020-03413-2'}, { 'key': '182104', 'doi-asserted-by': 'crossref', 'unstructured': "O'Donnell VB, Thomas D, Stanton R, Maillard J-Y, Murphy R, Jones S, et " 'al. Potential Role of Oral Rinses Targeting the Viral Lipid Envelope in ' 'SARS-CoV-2 Infection. Function (Oxf). 2020; 1(1): zqaa002.', 'DOI': '10.1093/function/zqaa002'}, { 'key': '182105', 'doi-asserted-by': 'crossref', 'unstructured': 'Popkin D, Zilka S, Dimaano M, Fujioka H, Rackley C, Salata R, et al. ' 'Cetylpyridinium Chloride (CPC) Exhibits Potent, Rapid Activity Against ' 'Influenza Viruses in vitro and in vivo. Pathog Immun. 2017; 2(2): ' '252-269.', 'DOI': '10.20411/pai.v2i2.200'}, { 'key': '182106', 'doi-asserted-by': 'crossref', 'unstructured': 'Shen L, Niu J, Wang C, Huang B, Wang W, Zhu N, et al. High-Throughput ' 'Screening and Identification of Potent Broad-Spectrum Inhibitors of ' 'Coronaviruses. J Virol. 2019; 93(12):e00023-19.', 'DOI': '10.1128/JVI.00023-19'}, { 'key': '182107', 'doi-asserted-by': 'crossref', 'unstructured': 'Lu X, Wang L, Sakthivel S, Whitaker B, Murray J, Kamili S et al. US CDC ' 'Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute ' 'Respiratory Syndrome Coronavirus 2. Emerg Infect Dis. 2020; ' '26(8):1654–1665.', 'DOI': '10.3201/eid2608.201246'}, { 'key': '182108', 'doi-asserted-by': 'crossref', 'unstructured': 'Mukherjee PK, Esper F, Buchheit K, Arters K, Adkins I, Ghannoum M, et ' 'al. Randomized, double-blind, placebo-controlled clinical trial to ' 'assess the safety and effectiveness of a novel dual-action oral topical ' 'formulation against upper respiratory infections. BMC Infect Dis. 2017; ' '17(1): 74.', 'DOI': '10.1186/s12879-016-2177-8'}, { 'key': '182109', 'doi-asserted-by': 'crossref', 'unstructured': 'Ellinger B, Bojkova D, Zaliani A, Cinati J, Claussen C, Westhaus S, et ' 'al. A SARS-CoV-2 cytopathicity dataset generated by high-content ' 'screening of a large drug repurposing collection. Sci Data. 2021; 8(1): ' '70.', 'DOI': '10.1038/s41597-021-00848-4'}, { 'key': '182110', 'doi-asserted-by': 'crossref', 'unstructured': 'Komine A, Yamaguchi E, Okamoto N, Yamamoto K. Virucidal activity of oral ' 'care products against SARS-CoV-2 in vitro. J Oral Maxillofac Surg Med ' 'Pathol. 2021; 10.1016/j.ajoms.2021.02.002.', 'DOI': '10.1016/j.ajoms.2021.02.002'}, { 'key': '182111', 'doi-asserted-by': 'crossref', 'unstructured': 'Meister T, Brüggemann Y, Todt D, Conzelmann C, Müller J, Groß R, et al. ' 'Virucidal Efficacy of Different Oral Rinses Against Severe Acute ' 'Respiratory Syndrome Coronavirus 2. J Infect Dis. 2020; ' '222(8):1289-1292.', 'DOI': '10.1093/infdis/jiaa471'}, { 'key': '182112', 'doi-asserted-by': 'crossref', 'unstructured': 'Koch-Heier J, Hoffmann H, Schindler M, Lussi A, Planz O. Inactivation of ' 'SARS-CoV-2 through Treatment with the Mouth Rinsing Solutions ViruProX® ' 'and BacterX® Pro. Microorganisms. 2021; 9(3):521.', 'DOI': '10.3390/microorganisms9030521'}, { 'key': '182113', 'doi-asserted-by': 'crossref', 'unstructured': 'Davies K, Buczkowski H, Welch SR, et al. Effective in vitro inactivation ' 'of SARS-CoV-2 by commercially available mouthwashes. J Gen Virol. ' '2021;102(4):10. 1099/jgv.0.001578.', 'DOI': '10.1099/jgv.0.001578'}, { 'key': '182114', 'doi-asserted-by': 'crossref', 'unstructured': 'Jain A, Grover V, Singh C, Sharma A, Das DK, Singh P, et al. ' 'Chlorhexidine: An effective anticovid mouth rinse. J Indian Soc ' 'Periodontol. 2021; 25(1):86-88.', 'DOI': '10.4103/jisp.jisp_824_20'}, { 'key': '182115', 'doi-asserted-by': 'crossref', 'unstructured': 'Anderson DE, Sivalingam V, Kang AEZ, Ananthanarayanan A, Arumugam H, ' 'Jenkins TM, et al. Povidone-Iodine Demonstrates Rapid in vitro Virucidal ' 'Activity Against SARS-CoV-2, The Virus Causing COVID-19 Disease. Infect ' 'Dis Ther. 2020; 9(3): 669-675.', 'DOI': '10.1007/s40121-020-00316-3'}, { 'key': '182116', 'doi-asserted-by': 'crossref', 'unstructured': 'Bidra AS, Pelletier JS, Westover JB, Frank S, Brown SM, Tessema B. ' 'Comparison of in vitro Inactivation of SARS CoV-2 with Hydrogen Peroxide ' 'and Povidone-Iodine Oral Antiseptic Rinses. J Prosthodont. 2020; 29(7): ' '599-603.', 'DOI': '10.1111/jopr.13220'}, { 'key': '182117', 'doi-asserted-by': 'crossref', 'unstructured': 'Gottsauner MJ, Michaelides I, Schmidt B, Scholz K, Buchalla W, Widbiller ' 'M, et al. A prospective clinical pilot study on the effects of a ' 'hydrogen peroxide mouthrinse on the intraoral viral load of SARS-CoV-2. ' 'Clin Oral Investig. 2020; 24(10): 3707-3713.', 'DOI': '10.1007/s00784-020-03549-1'}, { 'key': '182118', 'doi-asserted-by': 'crossref', 'unstructured': 'Martínez-Lamas L, Diz P, Pérez MT, Del Campo V, Cabrera JJ, López AM, et ' 'al. Is povidone iodine mouthwash effective against SARS-CoV-2? First in ' 'vivo tests. Oral Dis. 2020; 10.1111/odi.13526.', 'DOI': '10.1111/odi.13526'}, { 'key': '182119', 'doi-asserted-by': 'crossref', 'unstructured': 'Seneviratne C J, Balan P, Ko K, Udawatte N S, Lai D, Ng D, et al. ' 'Efficacy of commercial mouth-rinses on SARS-CoV-2 viral load in saliva: ' 'randomized control trial in Singapore. Infection. 2021; 49(2): 305-311.', 'DOI': '10.1007/s15010-020-01563-9'}, { 'key': '182120', 'doi-asserted-by': 'crossref', 'unstructured': 'Amirian ES. Potential fecal transmission of SARS-CoV-2: Current evidence ' 'and implications for public health. Int J Infect Dis. 2020; 95:363-370.', 'DOI': '10.1016/j.ijid.2020.04.057'}, { 'key': '182121', 'doi-asserted-by': 'crossref', 'unstructured': 'Wölfel R, Corman VM, Guggemos W, Seilmaier M, Zange S, Müller MA, et al. ' 'Virological assessment of hospitalized patients with COVID-2019. Nature. ' '2020; 581(7809): 465-469.', 'DOI': '10.1038/s41586-020-2196-x'}, { 'key': '182122', 'doi-asserted-by': 'crossref', 'unstructured': 'Wang XW, Li J, Guo T, Zhen B, Kong Q, Yi B, et al. Concentration and ' 'detection of SARS coronavirus in sewage from Xiao Tang Shan Hospital and ' "the 309th Hospital of the Chinese People's Liberation Army. Water Sci " 'Technol. 2005;52(8):213-221.', 'DOI': '10.2166/wst.2005.0266'}, { 'key': '182123', 'doi-asserted-by': 'crossref', 'unstructured': 'Alexandersen S, Chamings A, Bhatta TR. SARS-CoV-2 genomic and subgenomic ' 'RNAs in diagnostic samples are not an indicator of active replication. 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