Acetylsalicylic Acid and Salicylic Acid Inhibit SARS-CoV-2 Replication in Precision-Cut Lung Slices
et al., Vaccines, doi:10.3390/vaccines10101619 (In Vitro)
, Acetylsalicylic Acid and Salicylic Acid Inhibit SARS-CoV-2 Replication in Precision-Cut Lung Slices, Vaccines, doi:10.3390/vaccines10101619 (In Vitro)
In Vitro study showing that aspirin and metabolite salicylic acid inhibit SARS-CoV-2 viral replication in Vero, A549-ACE2, and Huh-7 cells, and in human precision-cut lung slices. Results indicate inhibition of post-entry pathways.
Geiger et al., 27 Sep 2022, peer-reviewed, 11 authors.
Contact:
jochen.bodem@uni-wuerzburg.de (corresponding author).
In Vitro studies are an important part of preclinical research, however results may be very different in vivo.
Acetylsalicylic Acid and Salicylic Acid Inhibit SARS-CoV-2 Replication in Precision-Cut Lung Slices
Vaccines, doi:10.3390/vaccines10101619
Aspirin, with its active compound acetylsalicylic acid (ASA), shows antiviral activity against rhino-and influenza viruses at high concentrations. We sought to investigate whether ASA and its metabolite salicylic acid (SA) inhibit SARS-CoV-2 since it might use similar pathways to influenza viruses. The compound-treated cells were infected with SARS-CoV-2. Viral replication was analysed by RTqPCR. The compounds suppressed SARS-CoV-2 replication in cell culture cells and a patient-near replication system using human precision-cut lung slices by two orders of magnitude. While the compounds did not interfere with viral entry, it led to lower viral RNA expression after 24 h, indicating that post-entry pathways were inhibited by the compounds.
Funding: Bayer Vital GmbH funded a part of this study. This publication was supported by the Open Access Publication Fund of the University of Wuerzburg.
Conflicts of Interest: Bayer Vital GmbH funded a part of this study but had no role in the design of the study; in the collection, analysis or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
References
Avota, Bodem, Chithelen, Mandasari, Beyersdorf et al., The Manifold Roles of Sphingolipids in Viral Infections, Front. Physiol, doi:10.3389/fphys.2021.715527
Chow, Khanna, Kethireddy, Yamane, Levine et al., Aspirin Use Is Associated With Decreased Mechanical Ventilation, Intensive Care Unit Admission, and In-Hospital Mortality in Hospitalized Patients with Coronavirus Disease, Anesth. Analg, doi:10.1213/ANE.0000000000005292
Di Bella, Luzzati, Principe, Zerbato, Meroni et al., Aspirin and Infection: A Narrative Review, Biomedicines, doi:10.3390/biomedicines10020263
Dong, Du, Gardner, An interactive web-based dashboard to track COVID-19 in real time, Lancet Infect. Dis, doi:10.1016/S1473-3099(20)30120-1
Geiger, Kersting, Schlegel, Stelz, Fahr et al., The Acid Ceramidase Is a SARS-CoV-2 Host Factor, Cells, doi:10.3390/cells11162532
Glatthaar-Saalmuller, Mair, Saalmuller, Antiviral activity of aspirin against RNA viruses of the respiratory tract-an in vitro study, Influenza Respir. Viruses, doi:10.1111/irv.12421
Goldman, Lye, Hui, Marks, Bruno et al., Remdesivir for 5 or 10 Days in Patients with Severe COVID-19, N. Engl. J. Med, doi:10.1056/NEJMoa2015301
Hoffmann, Mosbauer, Hofmann-Winkler, Kaul, Kleine-Weber et al., Chloroquine does not inhibit infection of human lung cells with SARS-CoV-2, Nature
Jancso, Cserepes, Gasz, Benko, Ferencz et al., Effect of acetylsalicylic acid on nuclear factor-kappaB activation and on late preconditioning against infarction in the myocardium, J. Cardiovasc. Pharm, doi:10.1097/01.fjc.0000175240.64444.68
Khoo, Fitzgerald, Fletcher, Ewings, Jaki et al., Optimal dose and safety of molnupiravir in patients with early SARS-CoV-2: A Phase I, open-label, dose-escalating, randomized controlled study, J. Antimicrob. Chemother, doi:10.1093/jac/dkab318
Kumar, Xin, Liang, Ly, Liang, NF-kappaB signaling differentially regulates influenza virus RNA synthesis, J. Virol, doi:10.1128/JVI.00909-08
Liu, Han, Blair, Kenst, Qin et al., SARS-CoV-2 Infects Endothelial Cells In Vivo and In Vitro, Front. Cell. Infect. Microbiol, doi:10.3389/fcimb.2021.701278
Liu, Huang, Li, Zhou, Liang et al., Effect of low-dose aspirin on mortality and viral duration of the hospitalized adults with COVID-19, Medicine, doi:10.1097/MD.0000000000024544
Maisonnasse, Guedj, Contreras, Behillil, Solas et al., Hydroxychloroquine use against SARS-CoV-2 infection in non-human primates, Nature, doi:10.1038/s41586-020-2558-4
Mazur, Wurzer, Ehrhardt, Pleschka, Puthavathana et al., Acetylsalicylic acid (ASA) blocks influenza virus propagation via its NF-kappaB-inhibiting activity, Cell Microbiol, doi:10.1111/j.1462-5822.2007.00902.x
Neuhaus, Danov, Konzok, Obernolte, Dehmel et al., Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices, J. Vis. Exp, doi:10.3791/57042
Nilsson-Payant, Uhl, Grimont, Doane, Cohen et al., The NF-kappaB Transcriptional Footprint Is Essential for SARS-CoV-2 Replication, J. Virol, doi:10.1128/JVI.01257-21
Oskotsky, Maric, Tang, Oskotsky, Wong et al., Mortality Risk Among Patients With COVID-19 Prescribed Selective Serotonin Reuptake Inhibitor Antidepressants, JAMA Netw. Open, doi:10.1001/jamanetworkopen.2021.33090
Perlman, Masters, Coronaviridae: The Viruses and Their Replication, Fields Virology
Schmidt, Lareau, Keshishian, Ganskih, Schneider et al., The SARS-CoV-2 RNA-protein interactome in infected human cells, Nat. Microbiol, doi:10.1038/s41564-020-00846-z
Wang, Cao, Zhang, Yang, Liu et al., Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro, Cell Res, doi:10.1038/s41422-020-0282-0
Zimniak, Kirschner, Hilpert, Geiger, Danov et al., The serotonin reuptake inhibitor Fluoxetine inhibits SARS-CoV-2 in human lung tissue, Sci. Rep, doi:10.1038/s41598-021-85049-0
Loading..
Please send us corrections, updates, or comments. Vaccines and
treatments are complementary. All practical, effective, and safe means should
be used based on risk/benefit analysis. No treatment, vaccine, or intervention
is 100% available and effective for all current and future variants. We do not
provide medical advice. Before taking any medication, consult a qualified
physician who can provide personalized advice and details of risks and
benefits based on your medical history and situation. FLCCC and WCH
provide treatment protocols.