Study of SARS-CoV-2 burden in whole mouth fluid and respiratory droplets with povidone iodine, hydrogen peroxide, and chlorhexidine mouthwashes in 36 hospitalized COVID-19 patients using PCR and rapid antigen testing. There were significant reductions in SARS-CoV-2 burden with all treatments in both respiratory droplets and whole mouth fluid.Analysis of short-term changes in viral load using PCR may not detect
effective treatments because PCR is unable to differentiate between intact
infectious virus and non-infectious or destroyed virus particles. For example
Alemany, Tarragó‐Gil perform RCTs with cetylpyridinium chloride
(CPC) mouthwash that show no difference in PCR viral load, however there was
significantly increased detection of SARS-CoV-2 nucleocapsid protein,
indicating viral lysis. CPC inactivates SARS-CoV-2 by degrading its membrane,
exposing the nucleocapsid of the virus. To better estimate changes in viral
load and infectivity, methods like viral culture or antigen detection that can
differentiate intact vs. degraded virus are preferred.
.Authors perform antigen testing for 6 hydrogen peroxide patients, showing that 50% became negative after treatment.
Abstract: medRxiv preprint doi: https://doi.org/10.1101/2021.02.25.21252488; this version posted March 1, 2021. The copyright holder for this preprint
(which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
It is made available under a CC-BY-NC-ND 4.0 International license .
Povidone iodine, hydrogen peroxide and chlorhexidine mouthwashes reduce SARS-CoV2
burden in whole mouth fluid and respiratory droplets
Bagavad Gita Jayaraman1*, Gunaseelan Rajan1, Priya Kannian2*, Chandra Lavanya4, Krittika
Ravichandran1, Nagalingeswaran Kumarasamy3, Kannan Ranganathan4, Veeraraghavan Aswini2,
Pasuvaraj Mahanathi2, Stephen Challacombe5, Jennifer Webster-Cyriaque6, Newell W
Johnson1,3,5,7
1
2
Chennai Dental Research Foundation, Chennai, India
VHS Laboratory Services, Department of Clinical Research, VHS Hospital, Chennai, India
3
VHS-Infectious Diseases Medical Centre, VHS Hospital, Chennai, India
4
Department of Oral Pathology, Ragas Dental College and Hospital, Chennai, India
5
Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, UK
6
University of North Carolina, Chapel Hill, USA
7
Menzies Health Institute Queensland, Griffith University, Queensland, Australia
Address for Correspondence:
* Dr. Bagavad Gita Jayaraman
Research Scientist
Chennai Dental Research Foundation
No. 56, Dr. R. K. Salai
6th Floor, Mylapore
Chennai – 600004
Tamil Nadu, India
Ph: 91-44-42103440
Email: gita70.geetha@gmail.com
* Dr. Priya Kannian
Scientist & Head
Department of Clinical Research
VHS Hospital
Rajiv Gandhi Salai
Taramani, Chennai – 600113
Tamil Nadu, India
Ph: 91-44-22541972
Email: priyakannian@gmail.com
Word count: 599 words
1
NOTE: This preprint reports new research that has not been certified by peer review and should not be used to guide clinical practice.
medRxiv preprint doi: https://doi.org/10.1101/2021.02.25.21252488; this version posted March 1, 2021. The copyright holder for this preprint
(which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
It is made available under a CC-BY-NC-ND 4.0 International license .
Please send us corrections, updates, or comments. Vaccines and
treatments are complementary. All practical, effective, and safe means should
be used based on risk/benefit analysis. No treatment, vaccine, or intervention
is 100% available and effective for all current and future variants. We do not
provide medical advice. Before taking any medication, consult a qualified
physician who can provide personalized advice and details of risks and
benefits based on your medical history and situation. FLCCC and WCH
provide treatment protocols.
