Abstract: medRxiv preprint doi: https://doi.org/10.1101/2021.02.25.21252488; this version posted March 1, 2021. The copyright holder for this preprint
(which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
It is made available under a CC-BY-NC-ND 4.0 International license .
Povidone iodine, hydrogen peroxide and chlorhexidine mouthwashes reduce SARS-CoV2
burden in whole mouth fluid and respiratory droplets
Bagavad Gita Jayaraman1*, Gunaseelan Rajan1, Priya Kannian2*, Chandra Lavanya4, Krittika
Ravichandran1, Nagalingeswaran Kumarasamy3, Kannan Ranganathan4, Veeraraghavan Aswini2,
Pasuvaraj Mahanathi2, Stephen Challacombe5, Jennifer Webster-Cyriaque6, Newell W
Johnson1,3,5,7
1
2
Chennai Dental Research Foundation, Chennai, India
VHS Laboratory Services, Department of Clinical Research, VHS Hospital, Chennai, India
3
VHS-Infectious Diseases Medical Centre, VHS Hospital, Chennai, India
4
Department of Oral Pathology, Ragas Dental College and Hospital, Chennai, India
5
Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, UK
6
University of North Carolina, Chapel Hill, USA
7
Menzies Health Institute Queensland, Griffith University, Queensland, Australia
Address for Correspondence:
* Dr. Bagavad Gita Jayaraman
Research Scientist
Chennai Dental Research Foundation
No. 56, Dr. R. K. Salai
6th Floor, Mylapore
Chennai – 600004
Tamil Nadu, India
Ph: 91-44-42103440
Email: gita70.geetha@gmail.com
* Dr. Priya Kannian
Scientist & Head
Department of Clinical Research
VHS Hospital
Rajiv Gandhi Salai
Taramani, Chennai – 600113
Tamil Nadu, India
Ph: 91-44-22541972
Email: priyakannian@gmail.com
Word count: 599 words
1
NOTE: This preprint reports new research that has not been certified by peer review and should not be used to guide clinical practice.
medRxiv preprint doi: https://doi.org/10.1101/2021.02.25.21252488; this version posted March 1, 2021. The copyright holder for this preprint
(which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
It is made available under a CC-BY-NC-ND 4.0 International license .
DOI record:
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"abstract": "<jats:p>SARS-CoV2 is transmitted primarily through oral mouth secretions and respiratory droplets. Commercial mouthwashes, povidone iodine (PI), hydrogen peroxide (HP) and chlorhexidine (CHX) have been tested in cell culture and RT-PCR studies for their efficacy to reduce SARS-CoV2 burden. Here, we evaluated SARS-CoV2 burden in whole mouth fluid (WMF) and respiratory droplets (RD) samples before and after the use of PI, HP or CHX mouthwashes in hospitalized COVID-19 patients using RT-PCR and rapid antigen test (RAT). Thirty-six SARS-CoV2 RT-PCR-positive in-patients were randomly assigned to one of the four groups: 20 and 60 minutes after 1% w/v PI or 1.5% HP; 90 and 180 minutes after 1.5% HP or 0.2% w/v CHX. WMF and RD samples were collected concurrently at baseline and after the two different time points. RD (92%) showed a higher reduction in SARS-CoV2 burden than WMF samples (50%; p=0.008). SARS-CoV2 burden was statistically lower at both 20 minutes (p=0.02) and 60 minutes (p=0.03) with PI; at 20 minutes with HP (p=0.0001); and 90 minutes with CHX (p=0.04). The overall and individual mean logarithmic reductions in the WMF and RD samples were greater than 1.0 at 20, 60 and 90 minutes after PI, HP or CHX. RAT-positive patients at 90 minutes post-treatment (n=3) demonstrated a one log increase in virus copies. Among the three RAT-negative post-treatment patients, SARS-CoV2 burden declined by one log in two while the third patient had a slight increase in RNA copies. In conclusion, we have shown for the first time that the mouthwashes, PI, HP and CHX can reduce the SARS-CoV2 burden in the concurrently collected RD and WMF samples. RAT is more appropriate than RT-PCR to evaluate the efficacy of the mouthwashes.</jats:p>",
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