Effect of Vitamin D3 supplementation vs. dietary-hygienic measures on SARS-COV-2 infection rates in hospital workers with 25-hydroxyvitamin D3 [25(OH)D3] levels ≥20 ng/mL
Maria Elena Romero-Ibarguengoitia, Dalia Gutiérrez-González, Carlos Cantú-López, Miguel Angel Sanz-Sánchez, Arnulfo González-Cantú
doi:10.1101/2022.07.12.22277450
Background. There is scant information on the effect of supplementation with vitamin D 3 in SARS-COV-2 infection cases when patient D3] levels are between 20-100ng/mL. Our aim was to evaluate the effect of supplementation with vitamin D 3 vs. dietary-hygienic measures on the SARS-COV-2 infection rate in participants with serum 25(OH)D 3 levels >20ng/mL. Methods. We invited hospital workers with 25(OH)D 3 levels between 20-100 ng/mL and no previous SARS-COV-2 infection; they were randomized as follows: treatment options were a) vitamin D 3 supplementation (52,000 IU monthly, G1) or b) dietary-hygienic measures (G2). We conducted a 3-to 6-month follow-up of SARS-COV-2 infections. Participants with 25(OH)D 3 levels <20 ng/mL were also analyzed. We divided these latter participants depending on whether they were supplemented (G3) or not (G4). Results. We analyzed 198 participants, with an average age of 44.4 (SD 9) years, and 130 (65.7%) were women. G1 had less cases of SARS-COV-2 infection than G2 after a follow-up of 3-to 6months (p<0.05). There were no differences between G3 and G4 at the 3-and 6-month follow-up cutoff points (p>0.05). Using mixed effect Cox regression analysis in 164 participants that completed six months of follow-up, vitamin D 3 supplementation appeared to act as a protective factor against SARS-COV-2 infection (HR 0.21, p=0.008) in G1 and G2. None of the participants treated with the supplementation doses had serum 25(OH)D 3 levels > 100ng/mL.
Conclusion. Vitamin D 3 supplementation in participants with 25(OH)D 3 levels between 20-100 ng/mL have a lower rate of SARS-COV-2 infection in comparison with the use of dietaryhygienic measures at six months follow-up.
Competing interests: The author(s) have no conflicts of interest to declare.
Ethics Statement: This research project was approved by the local IRB of the Universidad de Monterrey (Ref.
30062020-a-CN-CI ). An informed consent form was obtained from each participant. .
Disclosure The authors have no potential conflicts of interest associated with this study. Author Approval: all authors read an approved the final version of the manuscript. Protocol access: This study protocol can be reviewed in Clinicaltrials.gov. supplementation; G2: 25(OH)D3 >20 ng/mL without supplementation; G3: 25(OH)D3 <20 ng/mL with supplementation; G4 25(OH)D3 <20 ng/mL without supplementation.
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