ColdZyme® protects airway epithelia from infection with BA.4/5

DOI record: { "DOI": "10.1186/s12931-022-02223-2", "ISSN": [ "1465-993X" ], "URL": "http://dx.doi.org/10.1186/s12931-022-02223-2", "abstract": "<jats:title>Abstract</jats:title><jats:p>Vaccines against SARS-CoV-2 protect from critical or severe pathogenesis also against new variants of concern (VOCs) such as BA.4 and BA.5, but immediate interventions to avoid viral transmission and subsequent inflammatory reactions are needed. Here we applied the ColdZyme® medical device mouth spray to fully differentiated, polarized human epithelium cultured at an air-liquid interphase (ALI). We found using VOCs BA.1 and BA.4/5 that this device effectively blocked respiratory tissue infection. While infection with these VOCs resulted in intracellular complement activation, thus enhanced inflammation, and drop of transepithelial resistance, these phenomena were prevented by a single administration of this medical device. Thus, ColdZyme® mouth spray significantly shields epithelial integrity, hinders virus infection and blocks in a secondary effect intrinsic complement activation within airway cultures also in terms of the highly contagious VOCs BA.4/5. Crucially, our in vitro data suggest that ColdZyme® mouth spray may have an impact to protect against SARS-CoV-2 transmission, also in case of the Omicron BA.1, BA.4 and BA.5 variants.</jats:p>", "alternative-id": [ "2223" ], "article-number": "300", "assertion": [ { "group": { "label": "Article History", "name": "ArticleHistory" }, "label": "Received", "name": "received", "order": 1, "value": "23 August 2022" }, { "group": { "label": "Article History", "name": "ArticleHistory" }, "label": "Accepted", "name": "accepted", "order": 2, "value": "5 October 2022" }, { "group": { "label": "Article History", "name": "ArticleHistory" }, "label": "First Online", "name": "first_online", "order": 3, "value": "31 October 2022" }, { "group": { "label": "Article History", "name": "ArticleHistory" }, "label": "Change Date", "name": "change_date", "order": 4, "value": "24 January 2023" }, { "group": { "label": "Article History", "name": "ArticleHistory" }, "label": "Change Type", "name": "change_type", "order": 5, "value": "Correction" }, { "group": { "label": "Article History", "name": "ArticleHistory" }, "label": "Change Details", "name": "change_details", "order": 6, "value": "A Correction to this paper has been published:" }, { "URL": "https://doi.org/10.1186/s12931-023-02326-4", "group": { "label": "Article History", "name": "ArticleHistory" }, "label": "Change Details", "name": "change_details", "order": 7, "value": "https://doi.org/10.1186/s12931-023-02326-4" }, { "group": { "label": "Declarations", "name": "EthicsHeading" }, "name": "Ethics", "order": 1 }, { "group": { "label": "Ethical approval and consent to participate", "name": "EthicsHeading" }, "name": "Ethics", "order": 2, "value": "Written informed consent was obtained from all donors of leftover nasopharyngeal/ oropharyngeal specimens and EDTA blood by the participating clinics. The Ethics Committee of the Medical University of Innsbruck (a copy is attached to the proposal, ECS1166/2020) approved the use of anonymized leftover specimens of COVID-19 patients for scientific purposes. All authors read and approved the final manuscript." }, { "group": { "label": "Competing interests", "name": "EthicsHeading" }, "name": "Ethics", "order": 3, "value": "The study was partly financially supported by Enzymatica AB. The funders had no influence on study design, analysis and data validation. The major part of the study was supported by the FWF, Enzymatica only provided several materials." } ], "author": [ { "affiliation": [], "family": "Zaderer", "given": "Viktoria", "sequence": "first" }, { "affiliation": [], "family": "Dichtl", "given": "Stefanie", "sequence": "additional" }, { "affiliation": [], "family": "Bellmann Weiler", "given": "Rosa", "sequence": "additional" }, { "affiliation": [], "family": "Lass Flörl", "given": "Cornelia", "sequence": "additional" }, { "affiliation": [], "family": "Posch", "given": "Wilfried", "sequence": "additional" }, { "affiliation": [], "family": "Wilflingseder", "given": "Doris", "sequence": "additional" } ], "container-title": "Respiratory Research", "container-title-short": "Respir Res", "content-domain": { "crossmark-restriction": false, "domain": [ "link.springer.com" ] }, "created": { "date-parts": [ [ 2022, 10, 31 ] ], "date-time": "2022-10-31T19:02:54Z", "timestamp": 1667242974000 }, "deposited": { "date-parts": [ [ 2023, 1, 25 ] ], "date-time": "2023-01-25T07:02:52Z", "timestamp": 1674630172000 }, "funder": [ { "DOI": "10.13039/501100002428", "award": [ "P34070-B", "P33510-B" ], "doi-asserted-by": "publisher", "id": [ { "asserted-by": "publisher", "id": "10.13039/501100002428", "id-type": "DOI" } ], "name": "Austrian Science Fund" } ], "indexed": { "date-parts": [ [ 2025, 6, 9 ] ], "date-time": "2025-06-09T17:48:29Z", "timestamp": 1749491309696, "version": "3.37.3" }, "is-referenced-by-count": 9, "issue": "1", "issued": { "date-parts": [ [ 2022, 10, 31 ] ] }, "journal-issue": { "issue": "1", "published-online": { "date-parts": [ [ 2022, 12 ] ] } }, "language": "en", "license": [ { "URL": "https://creativecommons.org/licenses/by/4.0", "content-version": "tdm", "delay-in-days": 0, "start": { "date-parts": [ [ 2022, 10, 31 ] ], "date-time": "2022-10-31T00:00:00Z", "timestamp": 1667174400000 } }, { "URL": "https://creativecommons.org/licenses/by/4.0", "content-version": "vor", "delay-in-days": 0, "start": { "date-parts": [ [ 2022, 10, 31 ] ], "date-time": "2022-10-31T00:00:00Z", "timestamp": 1667174400000 } } ], "link": [ { "URL": "https://link.springer.com/content/pdf/10.1186/s12931-022-02223-2.pdf", "content-type": "application/pdf", "content-version": "vor", "intended-application": "text-mining" }, { "URL": "https://link.springer.com/article/10.1186/s12931-022-02223-2/fulltext.html", "content-type": "text/html", "content-version": "vor", "intended-application": "text-mining" }, { "URL": "https://link.springer.com/content/pdf/10.1186/s12931-022-02223-2.pdf", "content-type": "application/pdf", "content-version": "vor", "intended-application": "similarity-checking" } ], "member": "297", "original-title": [], "prefix": "10.1186", "published": { "date-parts": [ [ 2022, 10, 31 ] ] }, "published-online": { "date-parts": [ [ 2022, 10, 31 ] ] }, "publisher": "Springer Science and Business Media LLC", "reference": [ { "DOI": "10.1038/s41591-022-01911-2", "author": "H Tegally", "doi-asserted-by": "publisher", "journal-title": "Nat Med", "key": "2223_CR1", "unstructured": "Tegally H, et al. 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