Decoding the genome of SARS-CoV-2: a pathway to drug development through translation inhibition

Wu et al., RNA Biology, doi:10.1080/15476286.2024.2433830

Dec 2024

Review of the mechanism of SARS-CoV-2 protein translation and potential therapeutic strategies targeting viral RNA for inhibition. Protein translation is crucial for the entire intracellular life cycle of SARS-CoV-2, with genomic RNA immediately translated upon release into the cytoplasm to produce early non-structural proteins, followed by translation of subgenomic mRNAs to obtain structural proteins for virion assembly in the later stage. Authors discuss the current understanding of SARS-CoV-2 translation initiation, noting the roles of conserved RNA elements and untranslated regions in regulating translation. Strategies for blocking translation through targeted viral RNA cleavage (using antisense oligonucleotides, siRNA, CRISPR-Cas13) or inhibiting viral RNA element functions (using small molecule inhibitors) are highlighted.

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Wu et al., 4 Dec 2024, peer-reviewed, 4 authors. Contact: t-lxh@163.com.

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Decoding the genome of SARS-CoV-2: a pathway to drug development through translation inhibition

Shan-Na Wu, Ting Xiao, Hui Chen, Xiao-Hong Li

RNA Biology, doi:10.1080/15476286.2024.2433830

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the coronavirus disease 2019 (COVID-19) pandemic and is continuously spreading globally. The continuous emergence of new SARS-CoV-2 variants keeps posing threats, highlighting the need for fast-acting, mutation-resistant broadspectrum therapeutics. Protein translation is vital for SARS-CoV-2 replication, producing early nonstructural proteins for RNA replication and transcription, and late structural proteins for virion assembly. Targeted blocking of viral protein translation is thus a potential approach to developing effective anti-SARS-CoV-2 drugs. SARS-CoV-2, as an obligate parasite, utilizes the host's translation machinery. Translation-blocking strategies that target the SARS-CoV-2 mRNA, especially those that target its conserved elements are generally preferred. In this review, we discuss the current understanding of SARS-CoV-2 translation, highlighting the important conserved motifs and structures involved in its regulation. We also discuss the current strategies for blocking SARS-CoV-2 translation through viral RNA degradation or RNA element dysfunction.

References

Abbott, Dhamdhere, Liu, Development of CRISPR as an antiviral strategy to combat SARS-CoV-2 and influenza, Cell, doi:10.1016/j.cell.2020.04.020

Aldhumani, Hossain, Fairchild, RNA sequence and ligand binding alter conformational profile of SARS-CoV-2 stem loop II motif, Biochem Biophys Res Commun, doi:10.1016/j.bbrc.2021.01.013

Anokhina, Miller, Targeting ribosomal frameshifting as an antiviral strategy: from HIV-1 to SARS-CoV-2, Acc Chem Res, doi:10.1021/acs.accounts.1c00316

Babendure, Babendure, Ding, Control of mammalian translation by mRNA structure near caps, RNA, doi:10.1261/rna.2309906

Baldassarre, Paolini, Bruno, Potential use of noncoding RNAs and innovative therapeutic strategies to target the 5'UTR of SARS-CoV-2, Epigenomics, doi:10.2217/epi-2020-0162

Banerjee, Blanco, Bruce, SARS-CoV-2 disrupts splicing, translation, and protein trafficking to suppress host defenses, Cell, doi:10.1016/j.cell.2020.10.004

Baranov, Henderson, Anderson, Programmed ribosomal frameshifting in decoding the SARS-CoV genome, Virology, doi:10.1016/j.virol.2004.11.038

Berry, Waghray, Mortimer, Crystal structure of the HCV IRES central domain reveals strategy for start-codon positioning, Structure, doi:10.1016/j.str.2011.08.002

Bhatt, Scaiola, Loughran, Structural basis of ribosomal frameshifting during translation of the SARS-CoV-2 RNA genome, Science, doi:10.1126/science.abf3546

Brant, Tian, Majerciak, SARS-CoV-2: from its discovery to genome structure, transcription, and replication, Cell Biosci, doi:10.1186/s13578-021-00643-z

Chamond, Deforges, Ulryck, 40S recruitment in the absence of eIF4G/4A by EMCV IRES refines the model for translation initiation on the archetype of type II IRESs, Nucleic Acids Res, doi:10.1093/nar/gku720

Chen, Tarn, uORF-mediated translational control: recently elucidated mechanisms and implications in cancer, RNA Biol, doi:10.1080/15476286.2019.1632634

Condé, Allatif, Ohlmann, Translation of SARS-CoV-2 gRNA is extremely efficient and competitive despite a High degree of secondary structures and the presence of an uORF, Viruses, doi:10.3390/v14071505

Cortese, Lee, Cerikan, Integrative imaging reveals SARS-CoV-2-induced reshaping of subcellular morphologies, Cell Host Microbe, doi:10.1016/j.chom.2020.11.003

Coutard, Valle, De Lamballerie, The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade, Antiviral Res, doi:10.1016/j.antiviral.2020.104742

Crooke, Baker, Crooke, Antisense technology: an overview and prospectus, Nat Rev Drug Discov, doi:10.1038/s41573-021-00162-z

Crooke, Liang, Baker, Antisense technology: a review, J Biol Chem, doi:10.1016/j.jbc.2021.100416

De Breyne, Yu, Unbehaun, Direct functional interaction of initiation factor eIF4G with type 1 internal ribosomal entry sites, Proc Natl Acad Sci, doi:10.1073/pnas.0900153106

Dey, Yan, Schlick, Abolished frameshifting for predicted structure-stabilizing SARS-CoV-2 mutants: implications to alternative conformations and their statistical structural analyses, RNA, doi:10.1261/rna.080035.124

Dhorne-Pollet, Fitzpatrick, Costa, Antisense oligonucleotides targeting ORF1b block replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Front Microbiol, doi:10.3389/fmicb.2022.915202

Dinman, Mechanisms and implications of programmed translational frameshifting, Wiley Interdiscip Rev RNA, doi:10.1002/wrna.1126

Embarc-Buh, Francisco-Velilla, Martinez-Salas, RNA-Binding proteins at the host-pathogen interface targeting viral regulatory elements, Viruses, doi:10.3390/v13060952

Fareh, Zhao, Hu, Reprogrammed CRISPR-Cas13b suppresses SARS-CoV-2 replication and circumvents its mutational escape through mismatch tolerance, Nat Commun, doi:10.1038/s41467-021-24577-9

Fernández-Miragall, Salas, Structural organization of a viral IRES depends on the integrity of the GNRA motif, RNA, doi:10.1261/rna.5950603

Fields, Howley, De, Coronaviruses

Finkel, Gluck, Nachshon, SARS-CoV-2 uses a multipronged strategy to impede host protein synthesis, Nature, doi:10.1038/s41586-021-03610-3

Finkel, Mizrahi, Nachshon, The coding capacity of SARS-CoV-2, Nature, doi:10.1038/s41586-020-2739-1

Firth, Brierley, Non-canonical translation in RNA viruses, J Gen Virol, doi:10.1099/vir.0.042499-0

Franch, Gerdes, U-turns and regulatory RNAs, Curr Opin Microbiol, doi:10.1016/S1369-5274(00)00069-2

Frye, Cunningham, Mihailescu, Characterization of the SARS-CoV-2 genome 3 0 -untranslated region interactions with Host MicroRNAs, ACS Omega, doi:10.1021/acsomega.4c01050

Ganser, Kelly, Herschlag, The roles of structural dynamics in the cellular functions of RNAs, Nat Rev Mol Cell Biol, doi:10.1038/s41580-019-0136-0

Gerber, Donde, Matheson, Xnazymes targeting the SARS-CoV-2 genome inhibit viral infection, Nat Commun, doi:10.1038/s41467-022-34339-w

Gerresheim, Dünnes, Nieder-Röhrmann, microRNA-122 target sites in the hepatitis C virus RNA NS5B coding region and 3 0 untranslated region: function in replication and influence of RNA secondary structure, Cell Mol Life Sci, doi:10.1007/s00018-016-2377-9

Goebel, Miller, Bennett, A hypervariable region within the 3 0 cis -acting element of the murine coronavirus genome is nonessential for RNA synthesis but affects pathogenesis, J Virol, doi:10.1128/JVI.00803-06

Gorbalenya, Baker, Baric, The species severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2, Nat Microbiol, doi:10.1038/s41564-020-0695-z

Gross, Vicens, Einhorn, The IRES5 0 UTR of the dicistrovirus cricket paralysis virus is a type III IRES containing an essential pseudoknot structure, Nucleic Acids Res, doi:10.1093/nar/gkx622

Gutell, Cannone, Konings, Predicting U-turns in ribosomal RNA with comparative sequence analysis, J Mol Biol, doi:10.1006/jmbi.2000.3900

Hahn, Hahn, Rice, Conserved elements in the 3 0 untranslated region of flavivirus RNAs and potential cyclization sequences, J Mol Biol, doi:10.1016/0022-2836(87)90455-4

Haniff, Tong, Liu, Targeting the SARS-CoV-2 RNA genome with small molecule binders and ribonuclease targeting chimera (RIBOTAC) degraders, ACS Cent Sci, doi:10.1021/acscentsci.0c00984

Hartenian, Nandakumar, The molecular virology of coronaviruses, J Biol Chem, doi:10.1074/jbc.REV120.013930

Hashem, Des Georges, Dhote, Hepatitis-C-virus-like internal ribosome entry sites displace eIF3 to gain access to the 40S subunit, Nature, doi:10.1038/nature12658

Hegde, Tang, Zhao, Inhibition of SARS-CoV-2 by targeting conserved viral RNA structures and sequences, Front Chem, doi:10.3389/fchem.2021.802766

Hilgenfeld, Peiris, From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses, Antiviral Res, doi:10.1016/j.antiviral.2013.08.015

Hinnebusch, Ivanov, Sonenberg, Translational control by 5 0 -untranslated regions of eukaryotic mRNAs, Science, doi:10.1126/science.aad9868

Hoang, Luong, Ayun, A novel approach of antiviral drugs targeting viral genomes, Microorganisms, doi:10.3390/microorganisms10081552

Huang, Lokugamage, Rozovics, SARS coronavirus nsp1 protein induces template-dependent endonucleolytic cleavage of mRnas: viral mRNAs are resistant to nsp1-induced RNA cleavage, PloS Pathog, doi:10.1371/journal.ppat.1002433

Huston, Wan, Strine, Comprehensive in vivo secondary structure of the SARS-CoV-2 genome reveals novel regulatory motifs and mechanisms, Mol Cell, doi:10.1016/j.molcel.2020.12.041

Idris, Davis, Supramaniam, A SARS-CoV-2 targeted siRNA-nanoparticle therapy for COVID-19, Mol Ther, doi:10.1016/j.ymthe.2021.05.004

Imperatore, Cunningham, Pellegrene, Highly conserved s2m element of SARS-CoV-2 dimerizes via a kissing complex and interacts with host miRNA-1307-3p, Nucleic Acids Res, doi:10.1093/nar/gkab1226

Jaafar, Kieft, Viral RNA structure-based strategies to manipulate translation, Nat Rev Microbiol, doi:10.1038/s41579-018-0117-x

Jiang, Joshi, Gan, The highly conserved stem-loop II motif is dispensable for SARS-CoV-2, J Virol, doi:10.1128/jvi.00635-23

Johnstone, Bazzini, Giraldez, Upstream ORFs are prevalent translational repressors in vertebrates, Embo J, doi:10.15252/embj.201592759

Kean, The role of mRNA 5 0 -noncoding and 3 0 -end sequences on 40S ribosomal subunit recruitment, and how RNA viruses successfully compete with cellular mRNAs to ensure their own protein synthesis, Biol Cell, doi:10.1016/S0248-4900(03)00030-3

Kelly, Olson, Neupane, Structural and functional conservation of the programmed -1 ribosomal frameshift signal of SARS coronavirus 2 (SARS-CoV-2), J Biol Chem, doi:10.1074/jbc.AC120.013449

Khaitov, Nikonova, Shilovskiy, Silencing of SARS-CoV-2 with modified siRNA-peptide dendrimer formulation, Allergy, doi:10.1111/all.14850

Khan, Terenzi, Liu, A viral pan-end RNA element and host complex define a SARS-CoV-2 regulon, Nat Commun, doi:10.1038/s41467-023-39091-3

Kim, Kim, Park, A high-resolution temporal atlas of the SARS-CoV-2 translatome and transcriptome, Nat Commun, doi:10.1038/s41467-021-25361-5

Kim, Lee, Yang, The architecture of SARS-CoV-2 transcriptome, Cell, doi:10.1016/j.cell.2020.04.011

Kozak, Initiation of translation in prokaryotes and eukaryotes, Gene, doi:10.1016/S0378-1119(99)00210-3

Kozak, Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes, Cell, doi:10.1016/0092-8674(86)90762-2

Kozak, Pushing the limits of the scanning mechanism for initiation of translation, Gene, doi:10.1016/S0378-1119(02)01056-9

Kung, Lee, Chiang, Molecular virology of SARS-CoV-2 and related coronaviruses, Microbiol Mol Biol Rev, doi:10.1128/mmbr.00026-21

Kwan, Thompson, Noncanonical translation initiation in eukaryotes, Cold Spring Harb Perspect Biol, doi:10.1101/cshperspect.a032672

Lan, Allan, Malsick, Secondary structural ensembles of the SARS-CoV-2 RNA genome in infected cells, Nat Commun, doi:10.1038/s41467-022-28603-2

Lapointe, Grosely, Johnson, Dynamic competition between SARS-CoV-2 NSP1 and mRNA on the human ribosome inhibits translation initiation, Proc Natl Acad Sci, doi:10.1073/pnas.2017715118

Lee, Budhathoki, Lee, Broad-spectrum antiviral activity of 3D8, a nucleic acid-hydrolyzing single-chain variable fragment (scFv), targeting SARS-CoV-2 and multiple coronaviruses in vitro, Viruses, doi:10.3390/v13040650

Lei, Cheng, Wang, The influence of host miRNA binding to RNA within RNA viruses on virus multiplication, Front Cell Infect Microbiol, doi:10.3389/fcimb.2022.802149

Leppek, Das, Barna, Functional 5 0 UTR mRNA structures in eukaryotic translation regulation and how to find them, Nat Rev Mol Cell Biol, doi:10.1038/nrm.2017.103

Li, Callahan, Phadke, Automated flow synthesis of peptide-pna conjugates, ACS Cent Sci, doi:10.1021/acscentsci.1c01019

Li, Hilgenfeld, Whitley, Therapeutic strategies for COVID-19: progress and lessons learned, Nat Rev Drug Discov, doi:10.1038/s41573-023-00672-y

Li, Kang, Liu, Structural lability in stem-loop 1 drives a 5 0 UTR-3 0 UTR interaction in coronavirus replication, J Mol Biol, doi:10.1016/j.jmb.2008.01.068

Li, Sczepanski, Targeting a conserved structural element from the SARS-CoV-2 genome using l-DNA aptamers, RSC Chem Biol, doi:10.1039/D1CB00172H

Li, Structure-based design of antisense oligonucleotides that inhibit SARS-CoV-2 replication, bioRxiv

Li, Tang, Atlas of interactions between SARS-CoV-2 macromolecules and host proteins, Cell Insight, doi:10.1016/j.cellin.2022.100068

Li, Zhang, Zhang, LinearTurboFold: linear-time global prediction of conserved structures for RNA homologs with applications to SARS-CoV-2, Proc Natl Acad Sci U S A, doi:10.1073/pnas.2116269118

Lukavsky, Structure and function of HCV IRES domains, Virus Res, doi:10.1016/j.virusres.2008.06.004

Lulla, Wandel, Bandyra, Targeting the conserved stem loop 2 motif in the SARS-CoV-2 genome, J Virol, doi:10.1128/JVI.00663-21

Malone, Urakova, Snijder, Structures and functions of coronavirus replication-transcription complexes and their relevance for SARS-CoV-2 drug design, Nat Rev Mol Cell Biol, doi:10.1038/s41580-021-00432-z

Manfredonia, Nithin, Ponce-Salvatierra, Genome-wide mapping of SARS-CoV-2 RNA structures identifies therapeutically-relevant elements, Nucleic Acids Res, doi:10.1093/nar/gkaa1053

Martinez-Salas, Velilla, Fernandez-Chamorro, Insights into structural and mechanistic features of viral IRES elements, Front Microbiol, doi:10.3389/fmicb.2017.02629

Mathez, Cagno, Small molecules targeting viral RNA, Int J Mol Sci, doi:10.3390/ijms241713500

Mayr, What are 3 0 UTRs doing? Cold Spring, Harb Perspect Biol, doi:10.1101/cshperspect.a034728

Miao, Tidu, Eriani, Secondary structure of the SARS-CoV-2 5'-UTR, RNA Biology, doi:10.1080/15476286.2020.1814556

Morandi, Manfredonia, Simon, Genome-scale deconvolution of RNA structure ensembles, Nat Methods, doi:10.1038/s41592-021-01075-w

Nabiabad, Amini, Demirdas, Specific delivering of RNAi using spike's aptamer-functionalized lipid nanoparticles for targeting SARS-CoV-2: a strong anti-covid drug in a clinical case study, Chem Biol Drug Des, doi:10.1111/cbdd.13978

Niktab, Haghparast, Beigi, Design of advanced siRNA therapeutics for the treatment of COVID-19, Meta Gene, doi:10.1016/j.mgene.2021.100910

Ou, Liu, Lei, Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV, Nat Commun, doi:10.1038/s41467-020-15562-9

Pfafenrot, Schneider, Müller, Inhibition of SARS-CoV-2 coronavirus proliferation by designer antisense-circRNAs, Nucleic Acids Res, doi:10.1093/nar/gkab1096

Plant, Pérez-Alvarado, Jacobs, A three-stemmed mRNA pseudoknot in the SARS coronavirus frameshift signal, PLOS Biol, doi:10.1371/journal.pbio.0030172

Preiss, Hentze, From factors to mechanisms: translation and translational control in eukaryotes, Curr Opin Genet Dev, doi:10.1016/S0959-437X(99)00005-2

Qiao, Wotring, Zhang, Antisense oligonucleotides to therapeutically target SARS-CoV-2 infection, PLOS ONE, doi:10.1371/journal.pone.0281281

Ramos-Lorente, Berzal-Herranz, Romero-López, Recruitment of the 40S ribosomal subunit by the West Nile virus 3 0 UTR promotes the cross-talk between the viral genomic ends for translation regulation, Virus Res, doi:10.1016/j.virusres.2024.199340

Rasekhian, Roohvand, Habtemariam, The role of 3'UTR of RNA viruses on mRNA stability and translation enhancement, MRMC, doi:10.2174/1389557521666210217092305

Roberts, Langer, Wood, Advances in oligonucleotide drug delivery, Nat Rev Drug Discov, doi:10.1038/s41573-020-0075-7

Robertson, Igel, Baertsch, The structure of a rigorously conserved RNA element within the SARS virus genome, PLOS Biol, doi:10.1371/journal.pbio.0030005

Roman, Lewicka, Koirala, The SARS-CoV-2 programmed -1 ribosomal frameshifting element crystal structure solved to 2.09 Å using chaperone-assisted RNA crystallography, ACS Chem Biol, doi:10.1021/acschembio.1c00324

Rosenke, Leventhal, Moulton, Inhibition of SARS-CoV-2 in Vero cell cultures by peptide-conjugated morpholino oligomers, J Antimicrob Chemother, doi:10.1093/jac/dkaa460

Sajjanar, Raina, Untranslated regions (UTRs) orchestrate translation reprogramming in cellular stress responses, J Therm Biol, doi:10.1016/j.jtherbio.2017.02.006

Schlick, Zhu, Dey, To knot or not to knot: multiple conformations of the SARS-CoV-2 frameshifting RNA element, J Am Chem Soc, doi:10.1021/jacs.1c03003

Schoggins, Wilson, Panis, A diverse range of gene products are effectors of the type I interferon antiviral response, Nature, doi:10.1038/nature09907

Schubert, Karousis, Jomaa, SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation, Nat Struct Mol Biol, doi:10.1038/s41594-020-0511-8

Shang, Wan, Luo, Cell entry mechanisms of SARS-CoV-2, Proc Natl Acad Sci, doi:10.1073/pnas.2003138117

Slobodin, Sehrawat, Cap-independent translation and a precisely located RNA sequence enable SARS-CoV-2 to control host translation and escape anti-viral response, Nucleic Acids Res, doi:10.1093/nar/gkac615

Snijder, Bredenbeek, Dobbe, Unique and conserved features of genome and proteome of sars-coronavirus, an early split-off from the coronavirus group 2 lineage, J Mol Biol, doi:10.1016/S0022-2836(03)00865-9

Snijder, Limpens, De Wilde, A unifying structural and functional model of the coronavirus replication organelle: tracking down RNA synthesis, PLoS Biol, doi:10.1371/journal.pbio.3000715

Sola, Almazán, Zúñiga, Continuous and discontinuous RNA synthesis in coronaviruses, Annu Rev Virol, doi:10.1146/annurev-virology-100114-055218

Sorokin, Vassilenko, Terenin, Non-canonical translation initiation mechanisms employed by Eukaryotic viral mRNAs, Biochemistry (Mosc), doi:10.1134/S0006297921090042

Spahn, Mulder, Cryo-em visualization of a viral internal ribosome entry site bound to human ribosomes: the IRES functions as an RNA-based translation factor, Cell, doi:10.1016/j.cell.2004.08.001

Stern-Ginossar, Thompson, Mathews, Translational control in virus-infected cells, Cold Spring Harb Perspect Biol, doi:10.1101/cshperspect.a033001

Su, Ma, Feng, Efficient inhibition of SARS-CoV-2 using chimeric antisense oligonucleotides through RNase L activation*, Angew Chem Int Ed Engl, doi:10.1002/anie.202105942

Sun, Abriola, Niederer, Restriction of SARS-CoV-2 replication by targeting programmed -1 ribosomal frameshifting, Proc Natl Acad Sci, doi:10.1073/pnas.2023051118

Sun, Li, Ju, In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs, Cell, doi:10.1016/j.cell.2021.02.008

Szczesniak, Baliga-Gil, Jarmolowicz, Structural and functional RNA motifs of SARS-CoV-2 and influenza a virus as a target of viral inhibitors, Int J Mol Sci, doi:10.3390/ijms24021232

Tan, Yin, RNAi, a new therapeutic strategy against viral infection, Cell Res, doi:10.1038/sj.cr.7290248

Tengs, Kristoffersen, Bachvaroff, A mobile genetic element with unknown function found in distantly related viruses, Virol J, doi:10.1186/1743-422X-10-132

Thoms, Buschauer, Ameismeier, Structural basis for translational shutdown and immune evasion by the Nsp1 protein of SARS-CoV-2, Science, doi:10.1126/science.abc8665

Tidu, Janvier, Schaeffer, The viral protein NSP1 acts as a ribosome gatekeeper for shutting down host translation and fostering SARS-CoV-2 translation, RNA, doi:10.1261/rna.078121.120

Tolksdorf, Nie, Niemeyer, Inhibition of SARS-CoV-2 replication by a small interfering RNA targeting the leader sequence, Viruses, doi:10.3390/v13102030

Varricchio, Mathez, Pillonel, Geneticin shows selective antiviral activity against SARS-CoV-2 by interfering with programmed -1 ribosomal frameshifting, Antiviral Res, doi:10.1016/j.antiviral.2022.105452

Vora, Fontana, Mao, Targeting stem-loop 1 of the SARS-CoV-2 5 0 UTR to suppress viral translation and Nsp1 evasion, Proc Natl Acad Sci, doi:10.1073/pnas.2117198119

Wacker, Weigand, Akabayov, Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy, Nucleic Acids Res, doi:10.1093/nar/gkaa1013

Wang, Zhou, Wang, Rapid design and development of CRISPR-Cas13a targeting SARS-CoV-2 spike protein, Theranostics, doi:10.7150/thno.51479

Weinlich, Hüttelmaier, Schierhorn, IGF2BP1 enhances HCV ires-mediated translation initiation via the 3 0 UTR, RNA, doi:10.1261/rna.1578409

Wethmar, The regulatory potential of upstream open reading frames in eukaryotic gene expression, Wiley Interdiscip Rev RNA, doi:10.1002/wrna.1245

Wu, Luo, Developing effective siRNAs to reduce the expression of key viral genes of COVID-19, Int J Biol Sci, doi:10.7150/ijbs.59151

Yang, Olatunji, Rhodes, Discovery of small molecules targeting the frameshifting element RNA in SARS-CoV-2 viral genome, ACS Med Chem Lett, doi:10.1021/acsmedchemlett.3c00051

Yao, Sun, Chen, RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5 0 -untranslated region, Antiviral Res, doi:10.1016/j.antiviral.2022.105478

Yokoyama, Machida, Iwasaki, HCV IRES captures an actively translating 80S ribosome, Mol Cell, doi:10.1016/j.molcel.2019.04.022

Zafferani, Haddad, Luo, Amilorides inhibit SARS-CoV-2 replication in vitro by targeting RNA structures, Sci Adv, doi:10.1126/sciadv.abl6096

Zamore, Haley, Ribo-gnome: the big world of small RNAs, Science, doi:10.1126/science.1111444

Zeng, Liu, Nguyenla, Broad-spectrum crispr-mediated inhibition of SARS-CoV-2 variants and endemic coronaviruses in vitro, Nat Commun, doi:10.1038/s41467-022-30546-7

Zhang, Almazi, Ong, Nanoparticle delivery platforms for RNAi therapeutics targeting COVID-19 disease in the respiratory tract, Int J Mol Sci, doi:10.3390/ijms23052408

Zhang, Huang, Ren, Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2, Cell Res, doi:10.1038/s41422-021-00581-y

Zhang, Huang, Xie, In vivo structure and dynamics of the SARS-CoV-2 RNA genome, Nat Commun, doi:10.1038/s41467-021-25999-1

Zhang, Zheludev, Hagey, Cryo-em and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome, Nat Struct Mol Biol, doi:10.1038/s41594-021-00653-y

Zhou, Chen, Wang, Advances of CRISPR-Cas13 system in COVID-19 diagnosis and treatment, Genes Dis, doi:10.1016/j.gendis.2022.11.016

Zhu, Lee, Woo, An intranasal ASO therapeutic targeting SARS-CoV-2, Nat Commun, doi:10.1038/s41467-022-32216-0

Ziebuhr, Snijder, Gorbalenya, Virus-encoded proteinases and proteolytic processing in the Nidovirales, J Gen Virol, doi:10.1099/0022-1317-81-4-853

Ziv, Gabryelska, Lun, COMRADES determines in vivo RNA structures and interactions, Nat Methods, doi:10.1038/s41592-018-0121-0

Ziv, Price, Shalamova, The short-and long-range RNA-RNA interactome of SARS-CoV-2, Mol Cell, doi:10.1016/j.molcel.2020.11.004

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