Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes
Neal G Ravindra, Mia Madel Alfajaro, Victor Gasque, Nicholas C Huston, Han Wan, Klara Szigeti-Buck, Yuki Yasumoto, Allison M Greaney, Victoria Habet, Ryan D Chow, Jennifer S Chen, Jin Wei, Renata B Filler, Bao Wang, Guilin Wang, Laura E Niklason, Ruth R Montgomery, Stephanie C Eisenbarth, Sidi Chen, Adam Williams, Akiko Iwasaki, Tamas L Horvath, Ellen F Foxman, Richard W Pierce, Anna Marie Pyle, David Van Dijk, Craig B Wilen
PLOS Biology, doi:10.1371/journal.pbio.3001143
There are currently limited Food and Drug Administration (FDA)-approved drugs and vaccines for the treatment or prevention of Coronavirus Disease 2019 . Enhanced understanding of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and pathogenesis is critical for the development of therapeutics. To provide insight into viral replication, cell tropism, and host-viral interactions of SARS-CoV-2, we performed single-cell (sc) RNA sequencing (RNA-seq) of experimentally infected human bronchial epithelial cells (HBECs) in air-liquid interface (ALI) cultures over a time course. This revealed novel polyadenylated viral transcripts and highlighted ciliated cells as a major target at the onset of infection, which we confirmed by electron and immunofluorescence microscopy.
Supporting information S1
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'abstract': '<jats:p>There are currently limited Food and Drug Administration (FDA)-approved drugs and '
'vaccines for the treatment or prevention of Coronavirus Disease 2019 (COVID-19). Enhanced '
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'pathogenesis is critical for the development of therapeutics. To provide insight into viral '
'replication, cell tropism, and host–viral interactions of SARS-CoV-2, we performed '
'single-cell (sc) RNA sequencing (RNA-seq) of experimentally infected human bronchial '
'epithelial cells (HBECs) in air–liquid interface (ALI) cultures over a time course. This '
'revealed novel polyadenylated viral transcripts and highlighted ciliated cells as a major '
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'microscopy. Over the course of infection, the cell tropism of SARS-CoV-2 expands to other '
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