High-dose vitamin D supplementation is related to an improvement in serum alkaline phosphatase in COVID-19 patients; a randomized double-blinded clinical trial
Reza Rezvani Moghaddam, Zahra Khorasanchi, Ayad Rasool Noor, Mohammadreza Shadmand Foumani Moghadam, Ali Jafarzadeh Esfahani, Abdullah Khalaf Merhej Alyakobi, Mohammedhadi Lafta Alboresha, Payam Sharifan, Ali Bahari, Reza Rezvani, Malihe Aghasizade, Maryam Heshmati, Reza Assaran Darban, Gordon Ferns, Majid Ghayour Mobarhan
Journal of Health, Population and Nutrition, doi:10.1186/s41043-023-00409-y
Background The benefits and harms of vitamin D supplementation in the treatment of have not yet been fully documented. In this study, we aimed to evaluate the effects of high-dose vitamin D supplementation on liver function tests in COVID-19. Method This double-blinded randomized clinical trial was conducted on 140 hospitalized patients aged > 30 years. Patients were randomly allocated to receive either intervention group (n = 70 receiving 50,000 IU of vitamin D capsules orally as a single dose and then 10,000 IU syrup daily from the second day of admission for 30 days) and the control group (n = 70 receiving 1000 IU vitamin D syrup orally per day). Liver function tests (LFT), including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), and Lactate Dehydrogenase (LDH) were evaluated at baseline and at the end of the intervention. Decision tree analysis was performed to identify the predictors for change in liver enzymes.
Results Among COVID-19 patients, a significant decrease was observed in serum level of ALP between intervention and placebo groups (p = 0.04). In addition, decision tree analysis revealed that GGT, temperature, serum magnesium level at baseline and gender were the most important predictors of ALT changes in COVID-19 patients.
Conclusion High-dose vitamin D supplementation improved ALP markers among COVID-19 patients. More randomized controlled trials with longer follow-up times will be required.
Supplementary Information The online version contains supplementary material available at https:// doi. org/ 10. 1186/ s41043-023-00409-y. Additional file 1. Decision tree analysis to identify the predictors for change in AST and ALP enzymes in COVID-19 patients.
Author contributions MGHM and RAD initially conceptualized and designed the study, ZKH, AB, AJE and RRM upgraded the design. The manuscript was written by RR and ZKH. AJE were responsible for the design optimizing and statistical analysis. MLA, MH, MA, AKMA, ARN and MSHFM contribute sampling. GF performed English editing. All authors read and approved the final manuscript.
Declarations Ethics approval and consent to participate This study was approved by the Ethics Committee of MUMS (Mashhad University of Medical Sciences), Mashhad, Iran. (Ethic Number: IR.MUMS. REC.1399.237) and carried out based on the Declaration of Helsinki. Informed consent was obtained from all subjects involved in the study.
Consent for publication Not applicable as no personal data were used in this article.
Competing interests The authors declare no competing interests. • fast, convenient online submission • thorough peer review by experienced researchers in your field
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