Disinfection Effect of Hexadecyl Pyridinium Chloride on SARS-CoV-2 in vitro
Ke-Da Chen, Fei-Ke Ma, Qing-Jing Wang, Ying Wang, Xin-Yi Zhuang, Xu-Ning Zhang, Hai-Yan Mao, Yan-Jun Zhang
Intervirology, doi:10.1159/000526241
The novel coronavirus (COVID-19 or 2019-nCoV) is a respiratory virus that can exist in the mouth and saliva of patients and spreads through aerosol dispersion. Therefore, stomatological hospitals and departments have become high-infection-risk environments. Accordingly, oral disinfectants that can effectively inactivate the virus have become a highly active area of research. Hexadecyl pyridinium chloride, povidone-iodine, and other common oral disinfectants are the natural primary choices for stomatological hospitals. Therefore, this study investigated the inhibitory effect of hexadecyl pyridinium chloride on severe acute respiratory syndrome coronavirus (SARS-CoV-2) in vitro. Vero cells infected with SARS-CoV-2 were used to determine the disinfection effect; the CCK-8 method was used to determine cytotoxicity, and viral load was determined by real-time PCR. The results showed that hexadecyl pyridinium chloride has no obvious cytotoxic effect on Vero cells in the concentration range of 0.0125-0.05 mg/mL. The in vitro experiments showed that hexadecyl pyridinium chloride significantly inhibits the virus at concentrations of 0.1 mg/mL or above at 2 min of action. Thus, the results provide experimental support for the use of hexadecyl pyridinium chloride in stomatological hospitals.
After dilution (1:500), 200 µL was used for immunofluorescence analysis. When the concentration is 0.1 mg/mL, the virus is com-pletely inhibited. The infected cells were fixed. Then, anti-spike RBD Rabbit PAb (1:1,000; Sino Biological) was used as the antibody and Alexa Fluor488 ® -conjugated Goat Anti-rabbit IgG (1:1,500; Abcam) was used as the second antibody. The nuclei were stained with DAPI. The scale is 100 µm.
Statement of Ethics This article does not contain any studies with human participants or animals.
Conflict of Interest Statement All the authors declare that they have no conflict of interest.
Author Contributions Ke-da Chen, Fei-ke Ma, and Qing-jing Wang contributed to conception, design, data acquisition, and analysis and drafted and critically revised the manuscript; Ying Wang, Xin-yi Zhuang, and Xu-ning Zhang assisted with data management and data analysis; Hai-yan Mao and Yan-jun Zhang conceived and designed the study. All the authors gave final approval and agree to be accountable for all aspects of the work. Disinfection Effect of Hexadecyl Pyridinium Chloride on SARS-CoV-2
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"abstract": "<jats:p>The novel coronavirus (COVID-19 or 2019-nCoV) is a respiratory virus that can exist in the mouth and saliva of patients and spreads through aerosol dispersion. Therefore, stomatological hospitals and departments have become high-infection-risk environments. Accordingly, oral disinfectants that can effectively inactivate the virus have become a highly active area of research. Hexadecyl pyridinium chloride, povidone-iodine, and other common oral disinfectants are the natural primary choices for stomatological hospitals. Therefore, this study investigated the inhibitory effect of hexadecyl pyridinium chloride on severe acute respiratory syndrome coronavirus (SARS-CoV-2) in vitro. Vero cells infected with SARS-CoV-2 were used to determine the disinfection effect; the CCK-8 method was used to determine cytotoxicity, and viral load was determined by real-time PCR. The results showed that hexadecyl pyridinium chloride has no obvious cytotoxic effect on Vero cells in the concentration range of 0.0125–0.05 mg/mL. The in vitro experiments showed that hexadecyl pyridinium chloride significantly inhibits the virus at concentrations of 0.1 mg/mL or above at 2 min of action. Thus, the results provide experimental support for the use of hexadecyl pyridinium chloride in stomatological hospitals. </jats:p>",
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