HUMAN MONOCYTE STIMULATION BY EXPERIMENTAL WHOLE BODY HYPERTHERMIA STIMULIERUNG HUMANER MONOZYTEN DURCH EXPERIMENTELLE GANZKÖRPER HYPERTHERMIE
Maria Zellner, Nicole Hergovics, Erich Roth, Bernd Jilma, Andreas Spittler, Rudolf Oehler
The thermal effect of fever, an evolutionarily conserved acute-phase response, has been associated with improved survival and shortened disease duration in infection. The molecular consequence of this beneficial fever response is poorly understood. To study an influence of hyperthermia on human monocytes, which are important in recognition and elimination of pathogens, twelve healthy volunteers were immersed in a 39.5°C hot water bath to increase their body temperature. The expression of the endotoxin receptor CD14 and the complement receptor CD11b increased after the hot water bath (P<0.05), whereas the expression of the selectin CD62L, which mediate the initial attachment of leukocytes at endothelium during inflammation, was downregulated after hyperthermia (P<0.05). Comparable changes in monocyte receptor expression were observed after an in vitro hyperthermia. Furthermore, 3h after in vivo hyperthermia the response of monocytes to endotoxin was enhanced in an ex vivo lipopolysaccharide-stimulation-assay as shown by increased TNF-α release (P<0.05). We conclude that the thermal effect of fever leads directly to an activation of monocytes which increases their ability to respond to a bacterial challenge.
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