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Antiviral covalent ACE2 vesicle spray

Meng et al., Cell Biomaterials, doi:10.1016/j.celbio.2025.100017
Mar 2025  
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In Vitro and mouse study showing that covalently engineered ACE2 nanovesicle spray (nanoSpray) effectively neutralizes SARS-CoV-2 wild-type and Omicron variants. Authors developed a nasal spray containing ACE2 vesicles with an unnatural amino acid (fluorosulfate-L-tyrosine, FSY) that irreversibly binds to the spike protein of SARS-CoV-2. The FSY-ACE2-NVs demonstrated approximately 100-fold higher efficiency in blocking viral binding to host cells compared to soluble ACE2 proteins. The nanoSpray formulation with hyaluronic acid showed 20-fold improved lung retention in vivo. In K18-hACE2 transgenic mice infected with SARS-CoV-2, a single dose of nanoSpray significantly reduced viral loads, attenuated lung injury, and improved survival rates to 90% compared to 40% with non-covalent ACE2-NVs and 0% in untreated controls. The spray also effectively prevented Omicron variant infection when administered prophylactically.
Meng et al., 3 Mar 2025, China, peer-reviewed, 7 authors. Contact: zhangheng@szbl.ac.cn (corresponding author), zhangheng@szbl.ac.cn (corresponding author), lrao@szbl.ac.cn.
This PaperMiscellaneousAll
Antiviral covalent ACE2 vesicle spray
Qian-Fang Meng, Yu Han, Pan Pan, Yu Liu, Pan Pan, Rong-Chang Chen, Heng Zhang, Lang Rao
Cell Biomaterials, doi:10.1016/j.celbio.2025.100017
Highlights d Covalent ACE2 vesicles neutralized
DECLARATION OF INTERESTS L.R. and Q.-F.M. have applied for a patent related to this study. All other authors declare that they have no competing interests. SUPPLEMENTAL INFORMATION Supplemental information can be found online at https://doi.org/10.1016/j. celbio.2025.100017 . Supplemental information Antiviral covalent ACE2 vesicle spray Qian-Fang Meng, Yu Han, Yu Liu, Pan Pan, Rong-Chang Chen, Heng Zhang, and Lang Rao Supplemental Information Antiviral covalent ACE2 vesicle spray Qian-Fang Meng 1,5 , Yu Han 2,5 , Yu Liu 1,5 , Pan Pan 3,5 , Rong-Chang Chen 4 , Heng Zhang 3 School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, China. 4 Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510163, China. 5 These authors contributed equally. 6 Lead contact. * Correspondence: zhangheng@szbl.ac.cn (H.Z.); lrao@szbl.ac.cn (L.R.). Supplementary Figures and Tables
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