Analysis of blood and nasal epithelial transcriptomes to identify mechanisms associated with control of SARS-CoV-2 viral load in the upper respiratory tract
Mahdi Moradi Marjaneh, Joseph D Challenger, Antonio Salas, Alberto Gómez-Carballa, Abilash Sivananthan, Irene Rivero-Calle, Gema Barbeito-Castiñeiras, Cher Y Foo, Yue Wu, Felicity Liew, Heather R Jackson, Dominic Habgood-Coote, Giselle D’souza, Samuel J Nichols, Victoria J Wright, Michael Levin, Myrsini Kaforou, Ryan S Thwaites, Lucy C Okell, Federico Martinón-Torres, Aubrey J Cunnington
Journal of Infection, doi:10.1016/j.jinf.2023.10.009
Objectives: The amount of SARS-CoV-2 detected in the upper respiratory tract (URT viral load) is a key driver of transmission of infection. Current evidence suggests that mechanisms constraining URT viral load are different from those controlling lower respiratory tract viral J o u r n a l P r e -p r o o f mechanisms, including likely roles for NK cells, granulysin, prostanoids and interferon alpha-14. Inhibition of prostanoid production, and administration of interferon alpha-14 may be attractive transmission-blocking interventions.
accession E-MTAB-12791. NanoString nCounter data and corresponding metadata are available at https://github.com/MahdiMoradiMarjaneh/COVID19_viral_load. Codes used in the analyses can be accessed on the same GitHub repository.
Competing interests The authors declare that they have no competing interests. -0 .69 1 How many days after symptom onset the viral load was measured. 2 Cycle threshold value 3 Relative to the days of illness at the time of viral load measurement (see Figure 2D ) * These two samples are from the same subject The number of genes involved in each module. 2 Genes with the absolute value of module membership (the correlation between the module eigengene and gene expression values) > 0.9. Genes with negative module membership are marked with an asterisk. 3 No genes had module membership higher than 0.9 or lower than -0.9
Authors' contributions
Conceptualization
Declarations of interest: None.
Graphical
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