Abstract: Am J Blood Res 2021;11(1):44-52 www.AJBlood.us /ISSN:2160-1992/AJBR0128941 Original Article Seroprevalence of IgG against SARS-CoV-2 and its determinants among healthcare workers of a COVID-19 dedicated hospital of India Mala Mahto1, Ayan Banerjee1, Bijit Biswas2, Sushil Kumar1, Neeraj Agarwal2, Prabhat Kumar Singh3 Department of Biochemistry, All India Institute of Medical Sciences, Patna, Bihar, India; 2Department of Community and Family Medicine, All India Institute of Medical Sciences, Patna, Bihar, India; 3Department of Anaesthesiology and Critical Care, Director, All India Institute of Medical Sciences, Patna, Bihar, India 1 Received December 24, 2020; Accepted January 27, 2021; Epub February 15, 2021; Published February 28, 2021 Abstract: Healthcare workers (HCWs) due to their job profile are at utmost risk of contracting severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Serological survey is an useful tool for vulnerability mapping in an infectious disease pandemic. The aim of the current study was to assess seroprevalence of IgG against SARSCoV-2 and its determinants among HCWs of a tertiary healthcare facility of India. It was an observational study, cross-sectional in design conducted among 919 HCWs of All India Institute of Medical Sciences, Patna, Bihar, India during September, 2020. In results, IgG seroprevalence for SARS-CoV-2 among the study subjects was 13.3% [95% confidence interval (CI): 11.2-15.6%]. In univariate logistic regression analysis; gender, occupation, place of posting, use of full personal protective equipment (PPE), prior corona virus disease (COVID)-19 infection, influenza like illness (ILI), use of steam inhalation, consumption of azithromycin, zinc and vitamin C were the significant attributes which affected the IgG seropositivity for SARS-CoV-2. In the multivariable logistic regression model; occupation, place of posting, prior COVID-19 infection and ILI were significant determinants of IgG seropositivity for SARS-CoV-2. To conclude, majority of the HCWs were found to be IgG seronegative for SARS-CoV-2. Till availability of effective vaccine all of the HCWs should abide by infection prevention and control (IPC) measures to keep themselves and their contacts protected from SARS-CoV-2. Keywords: COVID-19, immunoglobulin G, seroepidemiologic studies, health personnel, epidemiologic factors