Comparative Bioavailability of Vitamin C After Short-Term Consumption of Raw Fruits and Vegetables and Their Juices: A Randomized Crossover Study

DOI record: { "DOI": "10.3390/nu17213331", "ISSN": [ "2072-6643" ], "URL": "http://dx.doi.org/10.3390/nu17213331", "abstract": "<jats:p>Background/Objectives: Vitamin C plays a vital role in human health, functioning as a powerful antioxidant and enzymatic cofactor. Although vitamin C bioavailability from food versus supplements has been debated, few studies have examined how intake form affects absorption and physiological markers. Methods: This randomized, controlled, crossover trial aimed to compare the bioavailability of vitamin C consumed as a supplement, through raw fruits and vegetables, or through fruit and vegetable juice. Twelve healthy adults underwent three 1-day crossover trials, each separated by a 2-week washout. Participants consumed 101.7 mg of vitamin C via powder, raw fruits and vegetables (186.8 g), or juice (200 mL). Plasma and urinary vitamin C concentrations, urinary metabolites (1H NMR), and antioxidant activity (ORAC and TRAP) were assessed over 24 h. Results: All interventions elevated plasma vitamin C levels, with juice yielding the highest AUC (25.3 ± 3.2 mg/dL·h). Urinary vitamin C increased in all groups. Metabolomics revealed increased urinary excretion of mannitol, glycine, taurine, dimethylglycine (DMG), and asparagine, and decreased choline and dimethylamine (DMA). Notably, urinary mannitol increased only in the morning. Choline significantly decreased after powder intake (p = 0.001), with similar trends observed in the other groups. DMG and glycine increased following raw and juiced vegetable intake. Antioxidant activity showed transient ORAC elevation post-powder but no sustained improvements. Conclusions: Vitamin C is bioavailable from all intake forms, with juice providing the most efficient absorption. Urinary metabolite changes suggest microbiota-related modulation, while antioxidant activity improvements were limited.</jats:p>", "alternative-id": [ "nu17213331" ], "author": [ { "ORCID": "https://orcid.org/0000-0003-4527-2475", "affiliation": [ { "name": "RISE Bio-Center, Kyungnam University, Changwon 51767, Republic of Korea" }, { "name": "Department of Food and Nutrition, Kyungnam University, Changwon 51767, Republic of Korea" } ], "authenticated-orcid": false, "family": "Choi", "given": "Mijoo", "sequence": "first" }, { "affiliation": [ { "name": "Department of Food and Nutrition, Kyungnam University, Changwon 51767, Republic of Korea" } ], "family": "Baek", "given": "Juha", "sequence": "additional" }, { "ORCID": "https://orcid.org/0000-0001-6044-0647", "affiliation": [ { "name": "Department of Food and Nutrition, Chonnam National University, Gwangju 61186, Republic of Korea" } ], "authenticated-orcid": false, "family": "Yun", "given": "Jung-Mi", "sequence": "additional" }, { "affiliation": [ { "name": "Department of Food and Nutrition, Chonnam National University, Gwangju 61186, Republic of Korea" } ], "family": "Hong", "given": "Young-Shick", "sequence": "additional" }, { "ORCID": "https://orcid.org/0000-0002-3462-6090", "affiliation": [ { "name": "Department of Food and Nutrition, Kyungnam University, Changwon 51767, Republic of Korea" } ], "authenticated-orcid": false, "family": "Park", "given": "Eunju", "sequence": "additional" } ], "container-title": "Nutrients", "container-title-short": "Nutrients", "content-domain": { "crossmark-restriction": false, "domain": [] }, "created": { "date-parts": [ [ 2025, 10, 23 ] ], "date-time": "2025-10-23T09:44:30Z", "timestamp": 1761212670000 }, "deposited": { "date-parts": [ [ 2025, 10, 25 ] ], "date-time": "2025-10-25T04:24:38Z", "timestamp": 1761366278000 }, "indexed": { "date-parts": [ [ 2025, 10, 25 ] ], "date-time": "2025-10-25T04:27:19Z", "timestamp": 1761366439861, "version": "build-2065373602" }, "is-referenced-by-count": 0, "issue": "21", "issued": { "date-parts": [ [ 2025, 10, 23 ] ] }, "journal-issue": { "issue": "21", "published-online": { "date-parts": [ [ 2025, 11 ] ] } }, "language": "en", "license": [ { "URL": "https://creativecommons.org/licenses/by/4.0/", "content-version": "vor", "delay-in-days": 0, "start": { "date-parts": [ [ 2025, 10, 23 ] ], "date-time": "2025-10-23T00:00:00Z", "timestamp": 1761177600000 } } ], "link": [ { "URL": "https://www.mdpi.com/2072-6643/17/21/3331/pdf", "content-type": "unspecified", "content-version": "vor", "intended-application": "similarity-checking" } ], "member": "1968", "original-title": [], "page": "3331", "prefix": "10.3390", "published": { "date-parts": [ [ 2025, 10, 23 ] ] }, "published-online": { "date-parts": [ [ 2025, 10, 23 ] ] }, "publisher": "MDPI AG", "reference": [ { "DOI": "10.1016/0003-9861(88)90093-8", "article-title": "Occurrence in Humans and Guinea Pigs of the Gene Related to Their Missing Enzyme L-Gulono-γ-Lactone Oxidase", "author": "Nishikimi", "doi-asserted-by": "crossref", "first-page": "842", "journal-title": "Arch. Biochem. Biophys.", "key": "ref_1", "volume": "267", "year": "1988" }, { "DOI": "10.1007/s12291-013-0375-3", "article-title": "Vitamin C in Disease Prevention and Cure: An Overview", "author": "Chambial", "doi-asserted-by": "crossref", "first-page": "314", "journal-title": "Indian J. Clin. Biochem.", "key": "ref_2", "volume": "28", "year": "2013" }, { "DOI": "10.1073/pnas.86.16.6377", "article-title": "Ascorbate Is an Outstanding Antioxidant in Human Blood Plasma", "author": "Frei", "doi-asserted-by": "crossref", "first-page": "6377", "journal-title": "Proc. Natl. Acad. Sci. USA", "key": "ref_3", "volume": "86", "year": "1989" }, { "DOI": "10.1006/abbi.1993.1074", "article-title": "The Pecking Order of Free Radicals and Antioxidants: Lipid Peroxidation, α-Tocopherol, and Ascorbate", "author": "Buettner", "doi-asserted-by": "crossref", "first-page": "535", "journal-title": "Arch. Biochem. Biophys.", "key": "ref_4", "volume": "300", "year": "1993" }, { "DOI": "10.1046/j.1523-5408.2002.00005.x", "article-title": "Vitamin C Function and Status in Chronic Disease", "author": "Jacob", "doi-asserted-by": "crossref", "first-page": "66", "journal-title": "Nutr. Clin. Care", "key": "ref_5", "volume": "5", "year": "2002" }, { "DOI": "10.1080/10717540601067786", "article-title": "Stability of Vitamins C and E in Topical Microemulsions for Combined Antioxidant Therapy", "author": "Rozman", "doi-asserted-by": "crossref", "first-page": "235", "journal-title": "Drug Deliv.", "key": "ref_6", "volume": "14", "year": "2007" }, { "DOI": "10.1093/jn/137.10.2171", "article-title": "New Developments and Novel Therapeutic Perspectives for Vitamin C", "author": "Li", "doi-asserted-by": "crossref", "first-page": "2171", "journal-title": "J. Nutr.", "key": "ref_7", "volume": "137", "year": "2007" }, { "DOI": "10.1093/ajcn/69.6.1086", "article-title": "Toward a New Recommended Dietary Allowance for Vitamin C Based on Antioxidant and Health Effects in Humans", "author": "Carr", "doi-asserted-by": "crossref", "first-page": "1086", "journal-title": "Am. J. Clin. Nutr.", "key": "ref_8", "volume": "69", "year": "1999" }, { "DOI": "10.1007/s00726-007-0555-7", "article-title": "SVCT1 and SVCT2: Key Proteins for Vitamin C Uptake", "author": "Savini", "doi-asserted-by": "crossref", "first-page": "347", "journal-title": "Amino Acids", "key": "ref_9", "volume": "34", "year": "2008" }, { "DOI": "10.1074/jbc.270.40.23706", "article-title": "Resolution of the Facilitated Transport of Dehydroascorbic Acid from Its Intracellular Accumulation as Ascorbic Acid", "author": "Vera", "doi-asserted-by": "crossref", "first-page": "23706", "journal-title": "J. Biol. Chem.", "key": "ref_10", "volume": "270", "year": "1995" }, { "DOI": "10.1023/A:1012186203165", "article-title": "Pharmacokinetic Model of Ascorbic Acid in Healthy Male Volunteers during Depletion and Repletion", "author": "Graumlich", "doi-asserted-by": "crossref", "first-page": "1133", "journal-title": "Pharm. Res.", "key": "ref_11", "volume": "14", "year": "1997" }, { "DOI": "10.7326/0003-4819-140-7-200404060-00010", "article-title": "Vitamin C Pharmacokinetics: Implications for Oral and Intravenous Use", "author": "Padayatty", "doi-asserted-by": "crossref", "first-page": "533", "journal-title": "Ann. Intern. Med.", "key": "ref_12", "volume": "140", "year": "2004" }, { "DOI": "10.1073/pnas.93.8.3704", "article-title": "Vitamin C Pharmacokinetics in Healthy Volunteers: Evidence for a Recommended Dietary Allowance", "author": "Levine", "doi-asserted-by": "crossref", "first-page": "3704", "journal-title": "Proc. Natl. Acad. Sci. USA", "key": "ref_13", "volume": "93", "year": "1996" }, { "DOI": "10.1177/02601060221078161", "article-title": "Impact of the COVID-19 Pandemic on Food and Drink Consumption and Related Factors: A Scoping Review", "author": "Shimpo", "doi-asserted-by": "crossref", "first-page": "177", "journal-title": "Nutr. Health", "key": "ref_14", "volume": "28", "year": "2022" }, { "DOI": "10.1016/j.chroma.2012.04.067", "article-title": "Sequential Determination of Fat- and Water-Soluble Vitamins in Green Leafy Vegetables during Storage", "author": "Santos", "doi-asserted-by": "crossref", "first-page": "179", "journal-title": "J. Chromatogr. A", "key": "ref_15", "volume": "1261", "year": "2012" }, { "DOI": "10.1248/bpb.27.1093", "article-title": "Effects of Oolong Tea on Plasma Antioxidative Capacity in Mice Loaded with Restraint Stress Assessed Using the Oxygen Radical Absorbance Capacity (ORAC) Assay", "author": "Kurihara", "doi-asserted-by": "crossref", "first-page": "1093", "journal-title": "Biol. Pharm. Bull.", "key": "ref_16", "volume": "27", "year": "2004" }, { "DOI": "10.1021/jf010586o", "article-title": "Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe", "author": "Ou", "doi-asserted-by": "crossref", "first-page": "4619", "journal-title": "J. Agric. Food Chem.", "key": "ref_17", "volume": "49", "year": "2001" }, { "DOI": "10.1016/0076-6879(94)34095-1", "article-title": "Total Antioxidant Status in Plasma and Body Fluids", "author": "Miller", "doi-asserted-by": "crossref", "first-page": "279", "journal-title": "Methods Enzymol.", "key": "ref_18", "volume": "234", "year": "1994" }, { "DOI": "10.1021/ac048630x", "article-title": "Statistical Total Correlation Spectroscopy: An Exploratory Approach for Latent Biomarker Identification from Metabolic 1H NMR Data Sets", "author": "Cloarec", "doi-asserted-by": "crossref", "first-page": "1282", "journal-title": "Anal. Chem.", "key": "ref_19", "volume": "77", "year": "2005" }, { "DOI": "10.1093/ajcn/86.5.1687", "article-title": "Influence of Acute Phytochemical Intake on Human Urinary Metabolomic Profiles", "author": "Walsh", "doi-asserted-by": "crossref", "first-page": "1687", "journal-title": "Am. J. Clin. Nutr.", "key": "ref_20", "volume": "86", "year": "2007" }, { "DOI": "10.1093/ajcn/48.3.601", "article-title": "Comparative Bioavailability to Humans of Ascorbic Acid Alone or in a Citrus Extract", "author": "Vinson", "doi-asserted-by": "crossref", "first-page": "501", "journal-title": "Am. J. Clin. Nutr.", "key": "ref_21", "volume": "48", "year": "1988" }, { "DOI": "10.3390/nu5114451", "article-title": "A Randomized Steady-State Bioavailability Study of Synthetic versus Natural (Kiwifruit-Derived) Vitamin C", "author": "Carr", "doi-asserted-by": "crossref", "first-page": "4451", "journal-title": "Nutrients", "key": "ref_22", "volume": "5", "year": "2013" }, { "DOI": "10.3390/nu5114284", "article-title": "Synthetic or Food-Derived Vitamin C—Are They Equally Bioavailable?", "author": "Carr", "doi-asserted-by": "crossref", "first-page": "4284", "journal-title": "Nutrients", "key": "ref_23", "volume": "5", "year": "2013" }, { "DOI": "10.1128/AEM.02157-10", "article-title": "Metagenomic Analysis of Kimchi, a Traditional Korean Fermented Food", "author": "Jung", "doi-asserted-by": "crossref", "first-page": "2264", "journal-title": "Appl. Environ. Microbiol.", "key": "ref_24", "volume": "77", "year": "2011" }, { "DOI": "10.1111/1462-2920.13589", "article-title": "Formation of Propionate and Butyrate by the Human Colonic Microbiota", "author": "Louis", "doi-asserted-by": "crossref", "first-page": "29", "journal-title": "Environ. Microbiol.", "key": "ref_25", "volume": "19", "year": "2017" }, { "DOI": "10.3390/biomedicines11041048", "doi-asserted-by": "crossref", "key": "ref_26", "unstructured": "Qian, W., Li, M., Yu, L., Tian, F., Zhao, J., and Zhai, Q. (2023). Effects of Taurine on Gut Microbiota Homeostasis: An Evaluation Based on Two Models of Gut Dysbiosis. Biomedicines, 11." }, { "DOI": "10.3390/antiox10081278", "doi-asserted-by": "crossref", "key": "ref_27", "unstructured": "Otten, A.T., Bourgonje, A.R., Harmsen, H.J.M., Alizadeh, B.Z., Dijkstra, G., and Faber, K.N. (2021). Vitamin C Supplementation in Healthy Individuals Leads to Shifts of Bacterial Populations in the Gut—A Pilot Study. Antioxidants, 10." }, { "key": "ref_28", "unstructured": "Hazan, S., Dave, S., Papoutsis, A.J., Deshpande, N., Howell, M.C., and Martin, L.M. (Future Microbiol., 2022). Vitamin C Improves Gut Bifidobacteria in Humans, Future Microbiol., online ahead of print." }, { "DOI": "10.1038/nm.3145", "article-title": "Intestinal Microbiota Metabolism of L-Carnitine, a Nutrient in Red Meat, Promotes Atherosclerosis", "author": "Koeth", "doi-asserted-by": "crossref", "first-page": "576", "journal-title": "Nat. Med.", "key": "ref_29", "volume": "19", "year": "2013" }, { "DOI": "10.1038/nature09922", "article-title": "Gut Flora Metabolism of Phosphatidylcholine Promotes Cardiovascular Disease", "author": "Wang", "doi-asserted-by": "crossref", "first-page": "57", "journal-title": "Nature", "key": "ref_30", "volume": "472", "year": "2011" }, { "DOI": "10.1093/clinchem/45.4.459", "article-title": "Defect in Dimethylglycine Dehydrogenase, a New Inborn Error of Metabolism: NMR Spectroscopy Study", "author": "Moolenaar", "doi-asserted-by": "crossref", "first-page": "459", "journal-title": "Clin. Chem.", "key": "ref_31", "volume": "45", "year": "1999" }, { "DOI": "10.1186/s40168-021-01101-1", "doi-asserted-by": "crossref", "key": "ref_32", "unstructured": "Guzior, D.V., and Quinn, R.A. (2021). Review: Microbial Transformations of Human Bile Acids. Microbiome, 9." } ], "reference-count": 32, "references-count": 32, "relation": {}, "resource": { "primary": { "URL": "https://www.mdpi.com/2072-6643/17/21/3331" } }, "score": 1, "short-title": [], "source": "Crossref", "subject": [], "subtitle": [], "title": "Comparative Bioavailability of Vitamin C After Short-Term Consumption of Raw Fruits and Vegetables and Their Juices: A Randomized Crossover Study", "type": "journal-article", "update-policy": "https://doi.org/10.3390/mdpi_crossmark_policy", "volume": "17" }