Unravelling Vitamin B12 as a potential inhibitor against SARS-CoV-2: A computational approach
Medha Pandya, Sejal Shah, Tanzil Juneja, Amisha Patel, Ayushman Gadnayak, Sushma Dave, Kajari Das, Jayashankar Das
Informatics in Medicine Unlocked, doi:10.1016/j.imu.2022.100951
This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Ethical statement The study does not involve the animals.
Declaration of competing interest The authors declare no competing interests.
References
Abobaker, Alzwi, Ahaa, Overview of the possible role of vitamin C in management of COVID-19, Pharmacol Reports
Alsaadi, Jones, Membrane binding proteins of coronaviruses, Future Virol
Bae, Hk, The role of vitamin C, vitamin D, and selenium in immune system against COVID-19, Molecules
Baladia, Pizarro, Rada, Vitamin C for the treatment of COVID-19: A living systematic review, MedRxiv
Barchetta, Carotti, Labbadia, Gentilucci, Muda et al., Liver Vitamin D Receptor, CYP2R1, and CYP27A1 Expression: Relationship With Liver Histology and Vitamin D3 Levels in Patients With Nonalcoholic Steatohepatitis or Hepatitis C Virus,
doi:10.1002/hep.25930.JournalPre-proofBender, Hildt, E ff ect of Hepatitis Viruses on the Nrf2 / Keap1-Signaling Pathway and Its Impact on Viral Replication and, Pathogenesis
Bolton, Null, Vitamin B15 A Review and Update, Orthomol PSYCHIATRY
Chen, Liu, Guo, Emerging coronaviruses : Genome structure , replication , and pathogenesis,
doi:10.1002/jmv.25681Chen, Oezguen, Urvil, Ferguson, Dann et al., Regulation of protein-ligand binding affinity by hydrogen bond pairing
Coutard, Valle, Lamballerie X De, Canard, Seidah et al., The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade, Antiviral Res,
doi:10.1016/j.antiviral.2020.104742Das, None
De, Van, Falzarano, Munster, REVIEWS SARS and MERS : recent insights into emerging coronaviruses, Nat Publ Gr n,
doi:10.1038/nrmicro.2016.81Dhanalakshmi, None
Dofferhoff, Piscaer, Schurgers, Visser, Jong et al., Reduced vitamin K status as a potentially modifiable risk factor of severe COVID-19 Anton n
Fung, Yuen, A tug-of-war between severe acute respiratory syndrome coronavirus 2 and host antiviral defence : lessons from other pathogenic viruses
Gadnayak, Sushma, Das, Artificial neural network-based study predicts GS441524 as a potential inhibitor of SARS COV-2 activator protein furin: A polypharmacology approach, Appl Biochem Biotechnology,
doi:10.21203/rs.3.rs-513443/v1Gal-Tanamy, Bachmetov, Ravid, Koren, Erman et al., An Innate Antiviral Agent Suppressing Hepatitis C Virus in Human Hepatocytes,
doi:10.1002/hep.24575Guo, Cao, Hong, Tan, Chen, The origin, transmission and clinical therapies on coronavirus disease, COVID
Hezekiah, Icon, Ibraheem, Oladejo Ogunyemi, Clement Akinmoladun et al., Network analysis, sequence and structure dynamics of key proteins of coronavirus and human host, and molecular docking of selected phytochemicals of nine medicinal plants, J Biomol Struct Dyn,
doi:10.1080/07391102.2020.1794971J O U R N A L P R E, -p r o o f
Jariwalla, Sh, Antiviral and immunomodulatory activities of ascorbic acid, Subcell Biochem
Jovic, Ali, Ibrahim, Jessop, Tarassoli et al., Could Vitamins Help in the Fight Against COVID-19 ?
Junaid, Ejaz, Abdalla, Abosalif, Ullah et al., E ff ective Immune Functions of Micronutrients against SARS-CoV-2, Nutrients,
doi:10.3390/nu12102992Krieger, Darden, Nabuurs, Finkelstein, Making optimal use of empirical energy functions: Force-field parameterization in crystal space, Proteins,
doi:10.1002/prot.20251Kumar, Kancharla, Kumar, In silico virtual screening-based study of nutraceuticals predicts the therapeutic potentials of folic acid and its derivatives against, VirusDisease,
doi:10.1007/s13337-020-00643-6Laskowski, Swindells, LigPlot + : Multiple Ligand À Protein Interaction Diagrams for Drug Discovery
Merad, Martin, Pathological inflammation in patients with COVID-19: a key role for monocytes and macrophages, Nat Rev Immunol,
doi:10.1038/s41577-020-0331-4Morris, Huey, Lindstrom, Sanner, Belew et al., AutoDock4 and AutoDockTools4: Automated Docking with Selective Receptor Flexibility, J Comput Chem,
doi:10.1002/jcc.21256.AutoDock4Munshi, Hussein, Toraih, Elshazli, Jardak et al., Vitamin D insufficiency as a potential culprit in critical COVID -19 patients,
doi:10.1002/jmv.26360Nalla, Shah, A new era in molecular biology clustered regularly interspaced short palindromic repeats/cas9 technology: A brief understanding, Adv Hum Biol
Ou, Liu, Lei, Li, Mi et al., Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV, Nat Commun,
doi:10.1038/s41467-020-15562-9Pandya, None
Pandya, Rawal, Nanoinformatics: An Emerging Trend in Cancer Therapeutics, Nanobiotechnology. Nanobiotechnology Concepts Appl. Heal. Agric. Environ
Pandya, Shah, None
Ross, Vitamin A Deficiency and Retinoid Repletion Regulate the Antibody Response to Bacterial Antigens and the Maintenance of Natural Killer Cells 1, Clin Immunol Immunopathol
Roujian, Zhao, Li, Niu, Yang et al., Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding, Lancet
Santos, Jeane, Can vitamin B12 be an adjuvant to COVID-19 treatment?, GSC Biol Pharm Sci
Scavone, Brusco, Bertini, Sportiello, Rafaniello et al., Current pharmacological treatments for COVID-19 : What ' s next,
doi:10.1111/bph.15072Sejal, Molecular Landscape and Computational Screening of the Natural inhibitors against HPV16 E6 Oncoprotein, Asian Pacific J Cancer Prev
Sheybani, Dokoohaki, Negahdaripour, Dehdashti, Zolghadr, The Role of Folic Acid in the Management of Respiratory Disease The role of folic acid in the management of respiratory disease caused by COVID-19,
doi:10.26434/chemrxiv.12034980.v1Tanzil, Pandya, None
Trott, Olson, AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading, J Comput Chem,
doi:10.1002/jcc.21334.AutoDockTurakhia, Shah, Green synthesis of copper oxide nanoparticles: a promising approach in the development of antibacterial textiles, J Coatings Technol Res
Vishakha, None
Wu, Zhang, Furin, A Potential Therapeutic Target for COVID-19 Furin : A Potential Therapeutic Target for COVID-19, ISCIENCE,
doi:10.1016/j.isci.2020.101642{ 'indexed': {'date-parts': [[2022, 4, 21]], 'date-time': '2022-04-21T21:42:05Z', 'timestamp': 1650577325182},
'reference-count': 42,
'publisher': 'Elsevier BV',
'license': [ { 'start': { 'date-parts': [[2022, 4, 1]],
'date-time': '2022-04-01T00:00:00Z',
'timestamp': 1648771200000},
'content-version': 'tdm',
'delay-in-days': 0,
'URL': 'https://www.elsevier.com/tdm/userlicense/1.0/'},
{ 'start': { 'date-parts': [[2022, 4, 15]],
'date-time': '2022-04-15T00:00:00Z',
'timestamp': 1649980800000},
'content-version': 'vor',
'delay-in-days': 14,
'URL': 'http://creativecommons.org/licenses/by-nc-nd/4.0/'}],
'content-domain': {'domain': [], 'crossmark-restriction': False},
'short-container-title': ['Informatics in Medicine Unlocked'],
'published-print': {'date-parts': [[2022, 4]]},
'DOI': '10.1016/j.imu.2022.100951',
'type': 'journal-article',
'created': {'date-parts': [[2022, 4, 20]], 'date-time': '2022-04-20T21:37:03Z', 'timestamp': 1650490623000},
'page': '100951',
'source': 'Crossref',
'is-referenced-by-count': 0,
'title': ['Unravelling Vitamin B12 as a potential inhibitor against SARS-CoV-2: A computational approach'],
'prefix': '10.1016',
'author': [ { 'ORCID': 'http://orcid.org/0000-0002-7601-1297',
'authenticated-orcid': False,
'given': 'Medha',
'family': 'Pandya',
'sequence': 'first',
'affiliation': []},
{'given': 'Sejal', 'family': 'Shah', 'sequence': 'additional', 'affiliation': []},
{ 'ORCID': 'http://orcid.org/0000-0001-7735-6644',
'authenticated-orcid': False,
'given': 'Dhanalakshmi',
'family': 'M',
'sequence': 'additional',
'affiliation': []},
{'given': 'Tanzil', 'family': 'Juneja', 'sequence': 'additional', 'affiliation': []},
{'given': 'Amisha', 'family': 'Patel', 'sequence': 'additional', 'affiliation': []},
{'given': 'Ayushman', 'family': 'Gadnayak', 'sequence': 'additional', 'affiliation': []},
{'given': 'Sushma', 'family': 'Dave', 'sequence': 'additional', 'affiliation': []},
{'given': 'Kajari', 'family': 'Das', 'sequence': 'additional', 'affiliation': []},
{'given': 'Jayashankar', 'family': 'Das', 'sequence': 'additional', 'affiliation': []}],
'member': '78',
'reference': [ { 'key': '10.1016/j.imu.2022.100951_bib1',
'doi-asserted-by': 'crossref',
'DOI': '10.1016/j.isci.2020.101642',
'article-title': 'Furin : a potential therapeutic target for COVID-19 furin : a potential '
'therapeutic target for COVID-19',
'volume': '23',
'author': 'Wu',
'year': '2020',
'journal-title': 'iScience'},
{ 'key': '10.1016/j.imu.2022.100951_bib2',
'series-title': 'Emerging coronaviruses : Genome structure , replication , and '
'pathogenesis',
'first-page': '418',
'author': 'Chen',
'year': '2020'},
{ 'key': '10.1016/j.imu.2022.100951_bib3',
'volume': 'vol. 9',
'author': 'Fung',
'year': '2020'},
{ 'key': '10.1016/j.imu.2022.100951_bib4',
'doi-asserted-by': 'crossref',
'unstructured': 'Wit E De, Doremalen N Van, Falzarano D, Munster VJ. REVIEWS SARS and '
'MERS : recent insights into emerging coronaviruses. Nat Publ Gr n.d. '
'https://doi.org/10.1038/nrmicro.2016.81.',
'DOI': '10.1038/nrmicro.2016.81'},
{ 'key': '10.1016/j.imu.2022.100951_bib5',
'article-title': 'Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and '
'its immune cross-reactivity with SARS-CoV',
'author': 'Ou',
'year': '2019',
'journal-title': 'Nat Commun'},
{ 'key': '10.1016/j.imu.2022.100951_bib6',
'doi-asserted-by': 'crossref',
'first-page': '275',
'DOI': '10.2217/fvl-2018-0144',
'article-title': 'Membrane binding proteins of coronaviruses',
'volume': '14',
'author': 'Alsaadi',
'year': '2019',
'journal-title': 'Future Virol'},
{ 'key': '10.1016/j.imu.2022.100951_bib7',
'doi-asserted-by': 'crossref',
'unstructured': 'M Dhanalakshmi; Kajari Das; Medha Pandya; Sejal Shah ;Ayushman Gadnayak '
';Sushma Dave;Jayashankar Das ; Artificial neural network-based study '
'predicts GS441524 as a potential inhibitor of SARS COV-2 activator '
'protein furin: a polypharmacology approach.Appl Biochem Biotechnol. '
'https://doi.org/10.21203/rs.3.rs-513443/v1.',
'DOI': '10.21203/rs.3.rs-513443/v1'},
{ 'key': '10.1016/j.imu.2022.100951_bib8',
'doi-asserted-by': 'crossref',
'first-page': '565',
'DOI': '10.1016/S0140-6736(20)30251-8',
'article-title': 'Genomic characterisation and epidemiology of 2019 novel coronavirus: '
'implications for virus origins and receptor binding',
'volume': '395',
'author': 'Roujian',
'year': '2020',
'journal-title': 'Lancet'},
{ 'key': '10.1016/j.imu.2022.100951_bib9',
'doi-asserted-by': 'crossref',
'first-page': '531',
'DOI': '10.1007/s11998-019-00303-5',
'article-title': 'Green synthesis of copper oxide nanoparticles: a promising approach in '
'the development of antibacterial textiles',
'volume': '1034',
'author': 'Turakhia',
'year': '2020',
'journal-title': 'J Coating Technol Res'},
{ 'key': '10.1016/j.imu.2022.100951_bib10',
'doi-asserted-by': 'crossref',
'first-page': '2461',
'DOI': '10.31557/APJCP.2021.22.8.2461',
'article-title': 'Molecular landscape and computational screening of the natural '
'inhibitors against HPV16 E6 oncoprotein',
'volume': '22',
'author': 'Tanzil',
'year': '2021',
'journal-title': 'Asian Pac J Cancer Prev APJCP'},
{ 'key': '10.1016/j.imu.2022.100951_bib11',
'series-title': 'Smart and intelligent vehicles for drug delivery: Theranostic '
'nanorobots. Adv. Nanomater. Point Care Diagnosis Ther.',
'first-page': '541',
'author': 'Dave',
'year': '2022'},
{ 'key': '10.1016/j.imu.2022.100951_bib12',
'first-page': '11',
'article-title': 'A new era in molecular biology clustered regularly interspaced short '
'palindromic repeats/cas9 technology: a brief understanding',
'author': 'Nalla',
'year': '2021',
'journal-title': 'Adv Hum Biol'},
{ 'key': '10.1016/j.imu.2022.100951_bib13',
'doi-asserted-by': 'crossref',
'first-page': '29',
'DOI': '10.1007/s13337-020-00643-6',
'article-title': 'In silico virtual screening-based study of nutraceuticals predicts the '
'therapeutic potentials of folic acid and its derivatives against',
'volume': '32',
'author': 'Kumar',
'year': '2021',
'journal-title': 'Virus Dis'},
{ 'key': '10.1016/j.imu.2022.100951_bib14',
'first-page': '1',
'article-title': 'Risk factors associated with acute respiratory distress syndrome and '
'death in patients with coronavirus disease 2019 pneumonia in Wuhan, '
'China',
'year': '2020',
'journal-title': 'JAMA Intern Med'},
{ 'key': '10.1016/j.imu.2022.100951_bib15',
'first-page': '6195',
'author': 'Omodele Ibraheem',
'year': '2021',
'journal-title': 'J Biomol Struct Dyn'},
{ 'key': '10.1016/j.imu.2022.100951_bib16',
'unstructured': 'Jovic TH, Ali SR, Ibrahim N, Jessop ZM, Tarassoli SP, Dobbs TD, et al. '
'Could vitamins help in the Fight against COVID-19 ? n.d..'},
{ 'key': '10.1016/j.imu.2022.100951_bib17',
'volume': 'vols. 1–37',
'author': 'Bender',
'year': '2019'},
{ 'key': '10.1016/j.imu.2022.100951_bib18',
'doi-asserted-by': 'crossref',
'first-page': '355',
'DOI': '10.1038/s41577-020-0331-4',
'article-title': 'Pathological inflammation in patients with COVID-19: a key role for '
'monocytes and macrophages',
'volume': '20',
'author': 'Merad',
'year': '2020',
'journal-title': 'Nat Rev Immunol'},
{ 'key': '10.1016/j.imu.2022.100951_bib19',
'series-title': 'Current pharmacological treatments for COVID-19 : what ’ s next ?',
'first-page': '4813',
'author': 'Scavone',
'year': '2020'},
{ 'key': '10.1016/j.imu.2022.100951_bib20',
'doi-asserted-by': 'crossref',
'first-page': '1517',
'DOI': '10.1007/s43440-020-00176-1',
'article-title': 'Overview of the possible role of vitamin C in management of COVID-19',
'volume': '72',
'author': 'Abobaker',
'year': '2020',
'journal-title': 'Pharmacol Rep'},
{ 'key': '10.1016/j.imu.2022.100951_bib21',
'doi-asserted-by': 'crossref',
'first-page': '5346',
'DOI': '10.3390/molecules25225346',
'article-title': 'The role of vitamin C, vitamin D, and selenium in immune system against '
'COVID-19',
'volume': '25',
'author': 'Bae',
'year': '2020',
'journal-title': 'Molecules'},
{ 'key': '10.1016/j.imu.2022.100951_bib22',
'doi-asserted-by': 'crossref',
'first-page': '215',
'DOI': '10.1007/978-1-4613-0325-1_11',
'article-title': 'Antiviral and immunomodulatory activities of ascorbic acid',
'author': 'Jariwalla',
'year': '1996',
'journal-title': 'Subcell Biochem'},
{ 'key': '10.1016/j.imu.2022.100951_bib23',
'first-page': '1',
'article-title': 'The origin, transmission and clinical therapies on coronavirus disease '
'2019 (COVID-19) outbreak–an update on the status',
'volume': '7',
'author': 'Guo',
'year': '2019',
'journal-title': 'Mil Med Res'},
{ 'key': '10.1016/j.imu.2022.100951_bib24',
'article-title': 'Can vitamin B12 be an adjuvant to COVID-19 treatment?',
'volume': '11',
'author': 'Santos',
'year': '2020',
'journal-title': 'GSC Biol Pharm Sci'},
{ 'key': '10.1016/j.imu.2022.100951_bib25',
'article-title': 'E ff ective immune functions of micronutrients against SARS-CoV-2',
'volume': '2',
'author': 'Junaid',
'year': '2020',
'journal-title': 'Nutrients'},
{ 'key': '10.1016/j.imu.2022.100951_bib26',
'doi-asserted-by': 'crossref',
'first-page': '2785',
'DOI': '10.1002/jcc.21256',
'article-title': 'AutoDock4 and AutoDockTools4: automated docking with selective receptor '
'flexibility',
'volume': '30',
'author': 'Morris',
'year': '2009',
'journal-title': 'J Comput Chem'},
{ 'key': '10.1016/j.imu.2022.100951_bib27',
'first-page': '455',
'article-title': 'AutoDock Vina: improving the speed and accuracy of docking with a new '
'scoring function, efficient optimization and multithreading',
'volume': '31',
'author': 'Trott',
'year': '2011',
'journal-title': 'J Comput Chem'},
{ 'key': '10.1016/j.imu.2022.100951_bib28',
'first-page': '2778',
'article-title': 'LigPlot + : multiple ligand À protein interaction diagrams for',
'author': 'Laskowski',
'year': '2011',
'journal-title': 'Drug Discov'},
{ 'key': '10.1016/j.imu.2022.100951_bib29',
'doi-asserted-by': 'crossref',
'first-page': '678',
'DOI': '10.1002/prot.20251',
'article-title': 'Making optimal use of empirical energy functions: force-field '
'parameterization in crystal space',
'volume': '57',
'author': 'Krieger',
'year': '2004',
'journal-title': 'Proteins'},
{ 'key': '10.1016/j.imu.2022.100951_bib30',
'doi-asserted-by': 'crossref',
'article-title': 'The spike glycoprotein of the new coronavirus 2019-nCoV contains a '
'furin- like cleavage site absent in CoV of the same clade',
'author': 'Coutard',
'year': '2020',
'journal-title': 'Antivir Res',
'DOI': '10.1016/j.antiviral.2020.104742'},
{ 'key': '10.1016/j.imu.2022.100951_bib31',
'series-title': 'The Role of Folic Acid in the Management of Respiratory Disease the role '
'of folic acid in the management of respiratory disease caused by '
'COVID-19',
'author': 'Covid- C',
'year': '2020'},
{ 'key': '10.1016/j.imu.2022.100951_bib32',
'series-title': 'Regulation of protein-ligand binding affinity by hydrogen bond pairing',
'author': 'Chen',
'year': '2016'},
{ 'key': '10.1016/j.imu.2022.100951_bib33',
'first-page': '260',
'article-title': 'Vitamin B15 A review and update',
'volume': '11',
'author': 'Bolton',
'year': '1982',
'journal-title': 'Orthomol Psychiatr'},
{ 'key': '10.1016/j.imu.2022.100951_bib34',
'series-title': 'Liver vitamin D receptor, CYP2R1, and CYP27A1 expression: relationship '
'with liver histology and vitamin D3 levels in patients with nonalcoholic '
'steatohepatitis or hepatitis C virus',
'author': 'Barchetta',
'year': '2012'},
{ 'key': '10.1016/j.imu.2022.100951_bib35',
'series-title': 'Vitamin D insufficiency as a potential culprit in critical COVID ‐ 19 '
'patients',
'first-page': '733',
'author': 'Munshi',
'year': '2021'},
{ 'key': '10.1016/j.imu.2022.100951_bib36',
'series-title': 'Vitamin D: an innate antiviral agent suppressing hepatitis C virus in '
'human hepatocytes',
'first-page': '1570',
'author': 'Gal-tanamy',
'year': '2011'},
{ 'key': '10.1016/j.imu.2022.100951_bib37',
'article-title': 'Vitamin C for the treatment of COVID-19: a living systematic review',
'author': 'Baladia',
'year': '2020',
'journal-title': 'medRxiv'},
{ 'key': '10.1016/j.imu.2022.100951_bib38',
'unstructured': 'Dofferhoff ASM, Piscaer I, Schurgers LJ, Visser MPJ, Jong PA De, Gosens '
'R, et al. Reduced vitamin K status as a potentially modifiable risk '
'factor of severe COVID-19 Anton n.d.'},
{ 'key': '10.1016/j.imu.2022.100951_bib39',
'doi-asserted-by': 'crossref',
'first-page': '63',
'DOI': '10.1006/clin.1996.0143',
'article-title': 'Vitamin A deficiency and retinoid repletion regulate the antibody '
'response to bacterial antigens and the maintenance of natural killer '
'cells 1',
'volume': '80',
'author': 'Ross',
'year': '1996',
'journal-title': 'Clin Immunol Immunopathol'},
{ 'key': '10.1016/j.imu.2022.100951_bib40',
'first-page': '115',
'volume': 'vol. 1141',
'author': 'Pandya',
'year': '2016'},
{ 'key': '10.1016/j.imu.2022.100951_bib41',
'series-title': 'Structural and functional analysis of AF9-MLL Oncogenic Fusion Protein '
'using homology modeling and simulation based approach',
'author': 'Dave',
'year': '2016'},
{ 'key': '10.1016/j.imu.2022.100951_bib42',
'first-page': '135',
'article-title': 'Nanoinformatics: an emerging trend in cancer therapeutics',
'author': 'Pandya',
'year': '2020',
'journal-title': 'Nanobiotechnol Nanobiotechnol Concepts Appl Heal Agric Environ'}],
'container-title': ['Informatics in Medicine Unlocked'],
'original-title': [],
'language': 'en',
'link': [ { 'URL': 'https://api.elsevier.com/content/article/PII:S2352914822000971?httpAccept=text/xml',
'content-type': 'text/xml',
'content-version': 'vor',
'intended-application': 'text-mining'},
{ 'URL': 'https://api.elsevier.com/content/article/PII:S2352914822000971?httpAccept=text/plain',
'content-type': 'text/plain',
'content-version': 'vor',
'intended-application': 'text-mining'}],
'deposited': { 'date-parts': [[2022, 4, 20]],
'date-time': '2022-04-20T21:37:28Z',
'timestamp': 1650490648000},
'score': 1,
'resource': {'primary': {'URL': 'https://linkinghub.elsevier.com/retrieve/pii/S2352914822000971'}},
'subtitle': [],
'short-title': [],
'issued': {'date-parts': [[2022, 4]]},
'references-count': 42,
'alternative-id': ['S2352914822000971'],
'URL': 'http://dx.doi.org/10.1016/j.imu.2022.100951',
'relation': {},
'ISSN': ['2352-9148'],
'issn-type': [{'value': '2352-9148', 'type': 'print'}],
'subject': ['Health Informatics'],
'published': {'date-parts': [[2022, 4]]},
'article-number': '100951'}
